Gram scale synthesis of QD450 core–shell quantum dots for cellular imaging and sorting

Vyshnava, Satyanarayana Swamy; Pandluru, Gayathri; Kumar, Kanderi Dileep; Panjala, Shiva Prasad; Banapuram, Swathi; Paramasivam, Kameshpandian; Anupalli, Roja Rani; Bontha, Rajashekar Reddy; Dowlatabad, Muralidhara Rao

doi:10.1007/s13204-020-01261-w

Cited by 13 publications

(12 citation statements)

References 59 publications

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“…CdS/ZnS with OA as capping are prepared as described in the established protocol 46 , 47 and labeled as , CdSe/ZnS with DPP as capping agent were prepared as described with minor modifications in the synthesis process 46 , 48 and labeled as ,. The CdSe/ZnS with TOP capped QD’s were prepared as described with minor modifications in the synthesis process 49 – 51 and labeled as .…”

Section: Methodsmentioning

confidence: 99%

“…QD’s are primarily modified with MPA through biphasic ligand exchange to attain active –COO - group on the surface of QD’s. These QD MPA are allowed for modification with NH 2 -(PEG) 8 -COOH (PEG) and finally conjugated with SA molecules with EDC/NHS coupling chemistry as mentioned in the earlier reports 46 . The conjugation of PEG and SA on the surface of QDs was successfully achieved by adding an equimolar mixture of 1 mM EDC and NHS which was prepared in the MES (2-(N-morpholino) ethane sulfonic acid) buffer at 4 °C (all the reactions are to be worked under the chilling condition to maintain the integrity of the PEG and SA).…”

Section: Methodsmentioning

confidence: 99%

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Quantum dots based in-vitro co-culture cancer model for identification of rare cancer cell heterogeneity

Vyshnava

Pandluru

Kumar

et al. 2022

Sci Rep

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Cancer cell heterogeneity (CCH) is crucial in understanding cancer progression and metastasis. The CCH is one of the stumbling blocks in modern medicine's therapeutics and diagnostics . An in-vitro model of co-culture systems of MCF-7, HeLa, HEK-293, with THP-1 cells showed the occurrence of EpCAM positive (EpCAM+) and EpCAM negative (EpCAM−) heterogenetic cancer cell types labeled with the Quantum Dot antibody conjugates (QDAb). This in-vitro model study could provide insights into the role of rare cancer cells manifestation and their heterogeneity in metastatic progression and risk for severe infections in these patients. We successfully report the presence of CCH based on the fluorescence ratios of the co-cultured cancer cells when treated with the QDAb. These short-term mimic co-cultures give a compelling and quite associated model for assessing early treatment responses in various cancers.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Quantum dots based in-vitro co-culture cancer model for identification of rare cancer cell heterogeneity

Vyshnava

Pandluru

Kumar

et al. 2022

Sci Rep

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“…CdSe/ZnS/ZnS with OA capping are prepared as described in the established protocoll 28,35 nd labelled as QD 450 , CdSe/ZnS with DPP as capping agent are prepared as described with minor changes in the synthesis process 28,36 and labelled as QD 525 , CdSe/ZnS with TOP capped QDs are prepared as described with minor changes in the synthesis process [37][38][39] and labelled as QD 615 . To make QDs for bio application, the surface of the QDs is modi ed through ligand exchange by MPA following conjugation with nPEGc and SA modi cations with EDC/NHS coupling as mentioned in the previous reports 28 . The QDs with nPEGc-SA are puri ed by dialysis to remove any free/excess nPEGc and SA.…”

Section: Methodsmentioning

confidence: 99%

“…The synthesized nPEGc functionalized quantum dots (QDs) include QD 450 , QD 525 , and QD 615 based on respective uorescence intensities were puri ed and made EDC/NHS coupling to provide an active amide bond between the QDs to the streptavidin 28 . The successful streptavidin conjugation with reference to the uorescence intensities, respective QDs is represented in gure S1, where showed the relative uorescence intensities of blue, green, and red hues.…”

Section: Antibody Conjugation With Quantum Dotsmentioning

confidence: 99%

Quantum Dots Based in-Vitro Co-Culture Cancer Model for Identification of Rare Cancer Cell Heterogeneity

Pandluru

et al. 2021

Preprint

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Cancer cell heterogeneity (CCH) is a key element in understanding cancer progression and metastasis. CCH is one of the challenges in therapeutics and diagnostics stumbling block in modern medicine. An in-vitro model of co-culture systems of MCF-7, HeLa, HEK-293, with THP-1 cells showed the occurrence of CTCs like cells with EpCAM+ and other cancer cell heterogenetic types with the Quantum Dot antibody conjugates (QDAb). This in-vitro model study could provide insights into the role of rare cancer cells and heterogeneity in metastasis, as well as the severity of infections in these patients. We successfully reported the presence of CCH based on the fluorescence ratios of the co-culture cancer cells. These short-term mimic co-cultures give a compelling and quite associated model for estimating early treatment responses in various types of cancers.

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“…[43] Several reports have raised concerns about the toxicity of cadmium-based QDs in biological applications. [42,[44][45][46][47] The discovery of cadmium ion (Cd 2+ ) presence showed it to be the primary cause of cytotoxicity. To counteract the toxicity of QDs, a coating of inorganic material was utilized to coat the core QDs, preventing oxidation and photobleaching of the core cadmium.…”

Section: =Cysmentioning

confidence: 99%

Biocompatible Ni‐doped CdSe/ZnS semiconductor nanocrystals for cellular imaging and sorting

et al. 2022

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Quantum dots (QD) with chemical composition QD 650CdSe=ZnS_Ni were successfully synthesized using a hydrothermal method and chemical precipitation. The nanocrystalline phase of the nanostructures was isolated and characterized using X-ray diffraction (XRD). The mean crystalline size doped core/shell Ni-dopant range was 9.0 ± 2.0 nm. The ferromagnetic data revealed the magnetic behaviour of QD 650CdSe=ZnS_Ni . The optical absorption measurements of these QDs were in the UV-visible light range 200-800 nm for a band gap value of 2.11 eV for QD 650 CdSe=ZnS_Ni . This means that pure QD 650CdSe and QD 650 CdSe=ZnS_Ni underwent a redshift when compared with bulk CdSe. For QD 650CdSe=ZnS_Ni there was successful uptake by cell lines including HeLa and MCF-7 for bioimaging and sorting applications.

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Gram scale synthesis of QD450 core–shell quantum dots for cellular imaging and sorting

Cited by 13 publications

References 59 publications

Quantum dots based in-vitro co-culture cancer model for identification of rare cancer cell heterogeneity

Quantum dots based in-vitro co-culture cancer model for identification of rare cancer cell heterogeneity

Quantum Dots Based in-Vitro Co-Culture Cancer Model for Identification of Rare Cancer Cell Heterogeneity

Biocompatible Ni‐doped CdSe/ZnS semiconductor nanocrystals for cellular imaging and sorting

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