Gram-positive bacterial flora on the root surface of wheat (Triticum aestivum L.) grown under different soil conditions

Sato, Katsutoshi; Jiang, Hong-Ying

doi:10.1007/bf00336051

Cited by 7 publications

(2 citation statements)

References 10 publications

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“…The iron limitation is one of the major factors affecting the growth of maize. Arthrobacter is one of the abundant genera recorded from the rhizosphere of Z. mays and wheat from diverse soil types [43,44]. Therefore, long-term field-based experiments on the efficacy of A. globiformis BGDa404 on different graminaceous crop plants will help in determining its wide application in enhancing crop productivity under iron stress environment.…”

Section: Resultsmentioning

confidence: 99%

Increased iron‐stress resilience of maize through inoculation of siderophore‐producing Arthrobacter globiformis from mine

Sharma

Mishra

Rau

et al. 2015

J. Basic Microbiol.

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Iron deficiency is common among graminaceous crops. Ecologically successful wild grasses from iron-limiting habitats are likely to harbour bacteria which secrete efficient high-affinity iron-chelating molecules (siderophores) to solubilize and mobilize iron. Such siderophore-producing rhizobacteria may increase the iron-stress resilience of graminaceous crops. Considering this, 51 rhizobacterial isolates of Dichanthium annulatum from iron-limiting abandoned mine (∼84% biologically unavailable iron) were purified and tested for siderophore production; and efficacy of Arthrobacter globiformis inoculation to increase iron-stress resilience of maize and wheat was also evaluated. 16S rRNA sequence analyses demonstrated that siderophore-producing bacteria were taxonomically diverse (seven genera, nineteen species). Among these, Gram-positive Bacillus (eleven species) was prevalent (76.92%). A. globiformis, a commonly found rhizobacterium of graminaceous crops was investigated in detail. Its siderophore has high iron-chelation capacity (ICC: 13.0 ± 2.4 μM) and effectively dissolutes diverse iron-complexes (FeCl3 : 256.13 ± 26.56 μM/ml; Fe2 O3 red: 84.3 ± 4.74 μM/ml; mine spoil: 123.84 ± 4.38 μM/ml). Siderophore production (ICC) of A. globiformis BGDa404 also varied with supplementation of different iron complexes. In plant bioassay with iron-deficiency sensitive species maize, A. globiformis inoculation triggered stress-associated traits (peroxidase and proline) in roots, enhanced plant biomass, uptake of iron and phosphate, and protein and chlorophyll contents. However, in iron deficiency tolerant species wheat, growth improvement was marginal. The present study illustrates: (i) rhizosphere of D. annulatum colonizing abandoned mine as a "hotspot" of siderophore-producing bacteria; and (ii) potential of A. globiformis BGDa404 inoculation to increase iron-stress resilience in maize. A. globiformis BGDa404 has the potential to develop as bioinoculant to alleviate iron-stress in maize.

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Section: Resultsmentioning

confidence: 99%

Increased iron‐stress resilience of maize through inoculation of siderophore‐producing Arthrobacter globiformis from mine

Sharma

Mishra

Rau

et al. 2015

J. Basic Microbiol.

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“…Duineveld et al (5,6) also noted a high similarity among denaturing gradient gel electrophoresis patterns of rhizosphere samples from growing chrysanthemum; the degree of similarity demonstrated some correlation to sampling time, depending on the analysis of DNA or rRNA target sequences. Only an altered window of observation generated by the use of specific primers might reveal a stronger time-dependent stimulation of certain bacterial groups than has been observed (25,27). Furthermore, it must be kept in mind that more-pronounced shifts might occur during the first days after the emergence of the root from the seed, when the root surface appears to be a rapidly changing substrate (11).…”

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Variation of Microbial Communities in Soil, Rhizosphere, and Rhizoplane in Response to Crop Species, Soil Type, and Crop Development

Wieland¹,

Neumann²,

Backhaus³

2001

Appl Environ Microbiol

308

174

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We investigated the influence of plant species, soil type, and plant development time on the shaping of microbial communities in soil and in association with roots. The sample group consisted of a total of 32 microcosms in three habitats: soil, rhizosphere, and rhizoplane. Communities were represented by the patterns of a sequence-specific separation of rRNA target sequences. Effects of experimental parameters were classified by a cluster analysis of pattern similarities. The type of plant species (clover, bean, or alfalfa) had the greatest effect in plant-associated habitats and also affected soil patterns. Plant development had a minor habitatdependent effect that was partly obscured by replicate variation. The results stress the applicability of biased community representations in an analysis of induced variation.A great variety of abiotic and biotic factors shape soil-and plant-associated habitats and modify the compositions and activities of their microbial communities, which in turn bear upon the quality of their environment, the growth of plants, and the production of root exudates (2). Bacterial communities in rootassociated habitats respond with respect to density, composition, and activity to the abundance and great diversity of organic root exudates, eventually yielding plant species-specific microfloras which may also vary during plant development stages (3,4,17,18). Due in part to the scarcity of convenient methods for exploration, our understanding of the different degrees and dynamics of microbial community variation as induced by soil type, plant type, or plant development is limited so far (5, 18).As one step towards a better understanding of the relative variations of complex microbial communities in response to common conditions of agricultural practice, we studied the effects of plant type, soil type, and temporal development on the compositions and activities of microbial communities in soil and in association with leguminous plants. Therefore, we compared banding patterns of amplified 16S rRNA target sequences from soil, rhizosphere, and rhizoplane fractions by temperature gradient gel electrophoresis (TGGE). This technique offers a culture-independent method for tracking dominant bacterial populations in space and time (21). Unlike with the use of 16S ribosomal DNA target sequences for such investigations, the use of rRNA modifies the observation in favor of metabolically active microorganisms, because the abundance of ribosomes in a community can be viewed as a speciesdependent function of cell numbers and their growth rates (9,24,28,31). To the best of our knowledge, this study represents the first approach using culture-independent methods to systematically monitor the degree of variation of active, dominant bacterial populations in response to soil type, plant type, and the state of development of the plant.In detail, soil samples from two different agricultural field sites in Braunschweig, Lower Saxony, Germany, were used: BBA soil (loamy sand) and FAL soil (para brown earth, silty sa...

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Salt‐Tolerant Rhizobacteria: Plant Growth Promoting Traits and Physiological Characterization Within Ecologically Stressed Environments

Egamberdiyeva

Islam

2008

Plant‐Bacteria Interactions

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Gram-positive bacterial flora on the root surface of wheat (Triticum aestivum L.) grown under different soil conditions

Cited by 7 publications

References 10 publications

Increased iron‐stress resilience of maize through inoculation of siderophore‐producing Arthrobacter globiformis from mine

Increased iron‐stress resilience of maize through inoculation of siderophore‐producing Arthrobacter globiformis from mine

Variation of Microbial Communities in Soil, Rhizosphere, and Rhizoplane in Response to Crop Species, Soil Type, and Crop Development

Salt‐Tolerant Rhizobacteria: Plant Growth Promoting Traits and Physiological Characterization Within Ecologically Stressed Environments

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