Gram-negative periodontal pathogens and bacterial endotoxin in metallic orthodontic brackets with or without an antimicrobial agent: An in-vivo study

Filho, Paulo Nelson; Valdez, Remberto Marcelo Argandoña; Andrucioli, Marcela Cristina Damião; Saraiva, Maria da Conceição Pereira; Feres, Magda; Sorgi, Carlos Artério; Faccioli, Lúcia Helena

doi:10.1016/j.ajodo.2011.06.026

Cited by 25 publications

(26 citation statements)

References 17 publications

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“…For example, an MIC of 2 mg/L in a twofold dilution scheme may correspond to any concentration between 1 and 2 mg/L [23–26]; moreover, in vivo study showed that the two microorganisms represent a triggering or supporting factor for the periodontitis development in FOA patients [27]. …”

Section: Resultsmentioning

confidence: 99%

Oral Candida in Patients with Fixed Orthodontic Appliance: In Vitro Combination Therapy

Alhamadi

Al-Saigh

N.N.Al-dabagh

et al. 2017

BioMed Research International

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Background Fixed orthodontic appliance (FOA) increases the cariogenic microorganisms of mouth including candida. The aim was to evaluate the pharmacodynamic effects of some antibacterial drugs in combination with most applicable antifungal agents on candida isolated from patients with FOA. Methods Three antifungal agents (amphotericin B (AMB), ketoconazole (KET), and itraconazole (ITZ)) and three antibacterial drugs (ciprofloxacin (CIP), doxycycline (DOX), and metronidazole (MET)) with serial concentrations have been used and microdilution broth method has been done for single and combination therapy, then fungal growth was assessed spectrophotometrically, and the combinations were evaluated by bliss independent analysis. Results According to bliss independent interaction, the synergistic interactions depended on ΔE values that showed the best for CIP was with AMB (ΔE = 55.14) followed with KET (ΔE = 41.23) and lastly ITR (ΔE = 39.67) at CIP = 150 mg/L. DOX was optimal with KET (ΔE = 42.11) followed with AMB (ΔE = 40.77) and the lowest with ITR (ΔE = 9.12) at DOX = 75 mg/L. MET is the best with AMB (ΔE = 40.95) and then with ITR (ΔE = 35.45) and finally KET (ΔE = 15.15) at MET 200 mg/L. Moreover, usage of higher concentrations of antibacterial agents revealed inhibitory effects. Conclusion This study uncovers the optimum antibiotic combination therapy against cariogenic candida with FOA by usage of low therapeutic concentrations.

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Section: Resultsmentioning

confidence: 99%

Oral Candida in Patients with Fixed Orthodontic Appliance: In Vitro Combination Therapy

Alhamadi

Al-Saigh

N.N.Al-dabagh

et al. 2017

BioMed Research International

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“…Significant differences were observed in the E. coli levels on the In-Ovation®R self-ligating bracket. This is an invasive bacterium releasing LPS [36], which has a high affinity for metal brackets [37].…”

Section: Discussionmentioning

confidence: 99%

Microbial complexes levels in conventional and self-ligating brackets

et al. 2016

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“…[23][24][25]31 The use of PMMA containing or coating with antimicrobial agents is advocated to combat this problem. 8,10,[32][33] Thus, a broad-spectrum, antimicrobial resin without the risk of developing resistant species of bacteria is needed as appliances to prevent post-treatment adverse effects. In recent years, much focus has been given to the potential role of nanoparticles-containing or coating orthodontic appliances in inhibiting the growth of the cariogenic bacteria.…”

Section: Discussionmentioning

confidence: 99%

Effects of Baseplates of Orthodontic Appliances with in situ generated Silver Nanoparticles on Cariogenic Bacteria: A Randomized, Doubleblind Cross-over Clinical Trial

Ghorbanzadeh¹,

Pourakbari²,

Bahador³

2015

The Journal of Contemporary Dental Practice

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Aim Polymethyl-methacrylate (PMMA) is commonly used primarily for baseplates of orthodontic appliances (BOA). The activities of cariogenic bacteria in biofilm on these surfaces may contribute to dental caries, gingival inflammation and periodontal disease. The PMMA incorporated with nanoparticles of silver (NanoAg-I-PMMA) and NanoAg in situ in PMMA (NanoAg-IS-PMMA) have been shown to control the growth of cariogenic bacteria, but clinical trial of anti-cariogenic application of these novel materials in orthodontics has not been evaluated. The main aim of the study is to compare the clinical effectiveness of using NanoAg-IS-PMMA and NanoAg-I-PMMA for construction of new BOA in inhibiting the planktonic growth and biofilm formation of the cariogenic bacteria. Materials and methods Twenty four patients with a median age of 12.6 years (7-15) harboring Streptococcus mutans, Streptococcus sobrinus and Lactobacillus acidophilus as well as Lactobacillus casei participated in the randomized, doubleblind, cross-over study. The experimental BOA, NanoAg-ISBOA and NanoAg-I-BOA, contained 0.5% w/w NanoAg while the control BOA was standard PMMA. Antibacterial effect of NanoAg-IS-BOA and NanoAg-I-BOA was assessed against test cariogenic bacteria by planktonic and biofilm bacterial cells growth inhibition. Results The average levels of test cariogenic bacteria in saliva decreased about 2 to 70 fold (30.9-98.4%) compared to baseline depending on the microorganism type and test BOA. Biofilm inhibition analysis demonstrated that NanoAg-I-BOA and NanoAg-IS-BOA inhibited the biofilm of all test bacteria by 20.1 to 79.9% compared to BOA. NanoAg-IS-BOA had a strong anti-biofilm effect against S. mutans, S. sobrinus and L. casei. However, NanoAg-I-BOA showed only slight antibiofilm effects on test bacteria. Most notably, at all period of the clinical trial, NanoAg-IS-BOA showed a higher antibacterial activity than NanoAg-I-BOA. Conclusion Based on the novel data that presented here, the NanoAg-IS-BOA had strong antimicrobial activity in the planktonic phase and subsequent biofilm formation of the cariogenic bacteria. Clinical significance Wearing of NanoAg-IS-BOA has the potential to minimize dental plaque formation and caries during orthodontic treatment. How to cite this article Ghorbanzadeh R, Pourakbari B, Bahador A. Effects of Baseplates of Orthodontic Appliances with in situ generated Silver Nanoparticles on Cariogenic Bacteria: A Randomized, Double-blind Cross-over Clinical Trial. J Contemp Dent Pract 2015;16(4):291-298.

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Gram-negative periodontal pathogens and bacterial endotoxin in metallic orthodontic brackets with or without an antimicrobial agent: An in-vivo study

Cited by 25 publications

References 17 publications

Oral Candida in Patients with Fixed Orthodontic Appliance: In Vitro Combination Therapy

Oral Candida in Patients with Fixed Orthodontic Appliance: In Vitro Combination Therapy

Microbial complexes levels in conventional and self-ligating brackets

Effects of Baseplates of Orthodontic Appliances with in situ generated Silver Nanoparticles on Cariogenic Bacteria: A Randomized, Doubleblind Cross-over Clinical Trial

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