2023
DOI: 10.1093/plphys/kiad079
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Grain shattering by cell death and fracture in Eragrostis tef

Abstract: Abscission, known as shattering in crop species, is a highly regulated process by which plants shed parts. Although shattering has been studied extensively in cereals and a number of regulatory genes have been identified, much diversity in the process remains to be discovered. Teff (Eragrostis tef) is a crop native to Ethiopia that is potentially highly valuable worldwide for its nutritious grain and drought tolerance. Previous work has suggested that grain shattering in Eragrostis might have little in common … Show more

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Cited by 2 publications
(3 citation statements)
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“…Samples for TEM were prepared as described in Yu et al . (2023) with modifications. Briefly, AZs at 31 and 38 d were dissected and fixed in 2% (w/v) PFA and 2% (w/v) glutaraldehyde with 0.1% (v/v) Tween 20 in 0.1 M cacodylate buffer at pH 7.4 overnight at 4°C and postfixed in 1% (w/v) osmium tetroxide in cacodylate buffer for 3 h, followed by 1% (w/v) aqueous uranyl acetate at 4°C overnight and then 50°C for 2 h. Samples were dehydrated in a graded acetone series and embedded in Quetol 651 epoxy resin at 60°C for 48 h. Samples were sectioned at 60 nm using a Leica Ultracut UCT ultramicrotome, mounted on formvar/carbon film on slotted gold grids (FCF2010‐Au‐SB; Electron Microscopy Sciences, Hatfield, PA, USA), stained with lead citrate for 8 min and imaged using a Talos L 120C G2 with a CETA 16 M 4 K × 4 K CMOS camera (Thermo Fisher Scientific), and tile mapped with 2 s exposure time using M aps 3 software (v.3.16) at 8500× (pixel size 1.72 nm) and with V elox (Release 3.1) at 17 500× or 22 000× magnifications.…”
Section: Methodsmentioning
confidence: 99%
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“…Samples for TEM were prepared as described in Yu et al . (2023) with modifications. Briefly, AZs at 31 and 38 d were dissected and fixed in 2% (w/v) PFA and 2% (w/v) glutaraldehyde with 0.1% (v/v) Tween 20 in 0.1 M cacodylate buffer at pH 7.4 overnight at 4°C and postfixed in 1% (w/v) osmium tetroxide in cacodylate buffer for 3 h, followed by 1% (w/v) aqueous uranyl acetate at 4°C overnight and then 50°C for 2 h. Samples were dehydrated in a graded acetone series and embedded in Quetol 651 epoxy resin at 60°C for 48 h. Samples were sectioned at 60 nm using a Leica Ultracut UCT ultramicrotome, mounted on formvar/carbon film on slotted gold grids (FCF2010‐Au‐SB; Electron Microscopy Sciences, Hatfield, PA, USA), stained with lead citrate for 8 min and imaged using a Talos L 120C G2 with a CETA 16 M 4 K × 4 K CMOS camera (Thermo Fisher Scientific), and tile mapped with 2 s exposure time using M aps 3 software (v.3.16) at 8500× (pixel size 1.72 nm) and with V elox (Release 3.1) at 17 500× or 22 000× magnifications.…”
Section: Methodsmentioning
confidence: 99%
“…Cell wall immunofluorescence followed the procedure described in Yu et al . (2023). To calculate average wall intensity for each probe, the probe signal in the AZ was divided by the area of cell wall autofluorescence using F iji (v.2.9.0; Schindelin et al ., 2012).…”
Section: Methodsmentioning
confidence: 99%
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