“Grafting-To” Covalent Binding of Plasmonic Nanoparticles onto Silica WGM Microresonators: Mechanically Robust Single-Molecule Sensors and Determination of Activation Energies from Single-Particle Events

Serrano, Mariana Paula; Subramanian, Sivaraman; Bilderling, Catalina von; Rafti, Matías; Vollmer, Frank

doi:10.3390/s23073455

Cited by 4 publications

(1 citation statement)

References 47 publications

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“…The remarkable sensitivity of these WGM optical resonances to environmental changes arises from their evanescent fields at the surface of a microsphere that extend to the interaction with the surrounding solution. Microsphere WGM sensors have been widely deployed as biosensors, enabling the detection of protein monolayers, biomolecular interactions, − bacteria, − and nanoparticles. − To push the boundary of detection sensitivity to its current highest single-molecule level to enable, for example, the detection of single atomic ions, plasmonic nanorods are strategically placed on the microspheres at the location of a WGM, as depicted in Figure a. These optoplasmonic WGM sensors capitalize on the evanescent field to excite plasmon resonance in plasmonic nanorods aligned with the electromagnetic field, leading to intensity enhancements at the nanorod tips; essentially placing the probing WGM field on the scale of the molecule, where only molecules interacting with the enhanced near-field region at the tips of the nanorods are detectable.…”

Section: Introductionmentioning

confidence: 99%

Whispering-Gallery Mode Optoplasmonic Microcavities: From Advanced Single-Molecule Sensors and Microlasers to Applications in Synthetic Biology

Houghton,

Kashanian,

Derrien

et al. 2024

ACS Photonics

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Optical microcavities, specifically, whispering-gallery mode (WGM) microcavities, with their remarkable sensitivity to environmental changes, have been extensively employed as biosensors, enabling the detection of a wide range of biomolecules and nanoparticles. To push the limits of detection down to the most sensitive single-molecule level, plasmonic nanorods are strategically introduced to enhance the evanescent fields of WGM microcavities. This advancement of optoplasmonic WGM sensors allows for the detection of single molecules of a protein, conformational changes, and even atomic ions, marking significant contributions in single-molecule sensing. This Perspective discusses the exciting research prospects in optoplasmonic WGM sensing of single molecules, including the study of enzyme thermodynamics and kinetics, the emergence of thermooptoplasmonic sensing, the ultrasensitive single-molecule sensing on WGM microlasers, and applications in synthetic biology.

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Section: Introductionmentioning

confidence: 99%

Whispering-Gallery Mode Optoplasmonic Microcavities: From Advanced Single-Molecule Sensors and Microlasers to Applications in Synthetic Biology

Houghton,

Kashanian,

Derrien

et al. 2024

ACS Photonics

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Detection of per- and polyfluoroalkyl water contaminants with a multiplexed 4D microcavities sensor

Saetchnikov,

Tcherniavskaia,

Saetchnikov

et al. 2023

Photon. Res.

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The per- and polyfluoroalkyl substances (PFAS) are a group of organofluorine chemicals treated as the emerging pollutants that are currently of particularly acute concern. These compounds have been employed intensively as surfactants over multiple decades and are already to be found in surface and ground waters at amounts sufficient to have an effect on human health and ecosystems. Because of the carbon–fluorine bonds, the PFAS have an extreme environmental persistence and their negative impact accumulates with further production and penetration into the environment. In Germany alone, more than thousands of sites have been identified as contaminated with PFAS; thus, timely detection of PFAS residue is becoming a high priority. In this paper, we report on the high performance optical detection method based on whispering gallery mode (WGM) microcavities applied for the first time to detect PFAS contaminants in aqueous solutions. A self-sensing boosted 4D microcavity fabricated with two-photon polymerization is employed as an individual sensing unit. In an example of the multiplexed imaging sensor with multiple hundreds of simultaneously interrogated microcavities we demonstrate the possibility to detect the PFAS chemicals representatives at a level down to 1 ppb (parts per billion).

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Optical Microcavities Empowered Biochemical Sensing: Status and Prospects

Guo,

Liang,

et al. 2024

Adv Devices Instrum

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Optical microcavities are compact structures that confine resonant photons in microscale dimensions for long periods of time, greatly enhancing light–matter interactions. Plentiful and profound physical mechanisms within these microcavities or functional microcavities have been extensively explored, including mode shift/splitting/broadening, lasing and gain enhancements, surface plasmon resonance, fluorescence resonance energy transferring, optical frequency comb spectroscopy, optomechanical interaction, and exceptional point. The versatility in design and the diverse range of materials, particularly composites involving metals and 2-dimensional materials, have paved a way for innovative approaches and improved performance in biochemical sensing applications. Leveraging the advantages ranging from miniaturization, high sensitivity, rapid response, and inherent stability, optical microcavity-based biochemical sensors have emerged to address the growing and increasingly complex demands of biochemical detection. This review commences with an exploration of fundamental mechanisms and structures and then delves into typical applications in recent advancements, covering the detection of biomacromolecules, cells, solid particles, liquid ions, and gas molecules. This review also culminates with a forward-looking perspective, highlighting future development trends and crucial research directions.

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“Grafting-To” Covalent Binding of Plasmonic Nanoparticles onto Silica WGM Microresonators: Mechanically Robust Single-Molecule Sensors and Determination of Activation Energies from Single-Particle Events

Cited by 4 publications

References 47 publications

Whispering-Gallery Mode Optoplasmonic Microcavities: From Advanced Single-Molecule Sensors and Microlasers to Applications in Synthetic Biology

Whispering-Gallery Mode Optoplasmonic Microcavities: From Advanced Single-Molecule Sensors and Microlasers to Applications in Synthetic Biology

Detection of per- and polyfluoroalkyl water contaminants with a multiplexed 4D microcavities sensor

Optical Microcavities Empowered Biochemical Sensing: Status and Prospects

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