Grafting of High-Density Poly(Ethylene Glycol) Monolayers on Si(111)

Zhu, Xiao Yang; Jun, Yongseok; Staarup, D. R.; Major, Ryan C.; Danielson, Susan J.; Boiadjiev, V.; Gladfelter, Wayne L.; Bunker, Bruce C.; Guo, Athena

doi:10.1021/la010672i

Cited by 96 publications

(111 citation statements)

References 20 publications

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“…[22] The limited success of physisorption and covalent grafting (the ''grafting to'' approach) in reducing protein adsorption has been attributed to the low surface density of PEG chains on the surface (<4 mg cm À2 ), [23] because of the excluded-volume effect. [24,25] A breakthrough in the development of protein-resistant materials came from the work of Prime and Whitesides, who showed that self-assembled monolayers (SAMs) of OEG-terminated alkanethiols (OEG-SH) confer protein resistance to gold, as determined by SPR. [16] Although OEG-SH SAMs on gold exhibited…”

Section: à2mentioning

confidence: 99%

In Pursuit of Zero: Polymer Brushes that Resist the Adsorption of Proteins

2009

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Protein resistant or “non‐fouling” surfaces are of great interest for a variety of biomedical and biotechnology applications. This article briefly reviews the development of protein resistant surfaces, followed by recent research on a new methodology to fabricate non‐fouling surfaces by surface‐initiated polymerization. We show that polymer brushes synthesized by surface‐initiated polymerization that present short oligo(ethylene glycol) side chains are exceptionally resistant to protein adsorption and cell adhesion. The importance of the protein and cell resistance conferred by these polymer brushes is illustrated by their use as substrates for the fabrication of antibody microarrays that exhibit femtomolar limits of detection in complex fluids such as serum and blood with relaxed requirements for intermediate wash steps. This example highlights the important point that the reduction in background noise afforded by protein‐resistant surfaces can greatly simplify the development of ultrasensitive heterogeneous, surface‐based clinical and proteomic assays with increased sensitivity and utility.

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Section: à2mentioning

confidence: 99%

In Pursuit of Zero: Polymer Brushes that Resist the Adsorption of Proteins

2009

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“…The solubility of salts in ethylene glycol (EG) is appreciable: 20.6 g and 7.5 g NaCl dissolve in 100 g EG at 25 [23]. Poly(ethylene glycol) grafted onto solid surfaces is also used to reduce protein adsorption [24].…”

Section: Biochemically Relevant Liquidsmentioning

confidence: 99%

Thermocapillary actuation of droplets on chemically patterned surfaces by programmable microheater arrays

Darhuber¹,

Valentino

Troian³

et al. 2003

J. Microelectromech. Syst.

214

167

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Abstract-We have designed a microfluidic device for the actuation of liquid droplets or continuous streams on a solid surface by means of integrated microheater arrays. The microheaters provide control of the surface temperature distribution with high spatial resolution. These temperature gradients locally alter the surface tension along droplets and thin films thus propelling the liquid toward the colder regions. In combination with liquophilic and liquophobic chemical surface patterning, this device can be used as a logistic platform for the parallel and automated routing, mixing and reacting of a multitude of liquid samples, including alkanes, poly(ethylene glycol) and water.[1024]

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“…This is an example of the 'grafting to' technique. Zhu et al [15] employed a two-step procedure to link the hydroxyl end-group of poly(ethylene oxide) molecules with an ether bond to a silicon surface. Using an even simpler method, Maas et al [16] linked vinyl-terminated polystyrene molecules to a silica surface by letting the vinyl groups react with the hydroxyl groups at a silica surface that appear as a result of the chemisorption of water to form ether bonds.…”

Section: Introductionmentioning

confidence: 99%

Characterization of poly(ethylene oxide) brushes on glass surfaces and adhesion of Staphylococcus epidermidis

Kaper

Busscher²,

Norde³

2003

Journal of Biomaterials Science, Polymer Edition

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Abstract-Poly(ethylene oxide) brushes have been covalently bound to glass surfaces and their presence was demonstrated by an increase in water contact angles from fully wettable on glass to advancing contact angles of 54 ± , with a hysteresis of 32 ± . In addition, electrophoretic mobilities of glass and brush-coated glass were determined using streaming potential measurements. The dependence of the electrophoretic mobilities on the ionic strength was analyzed in terms of a softlayer model, yielding an electrophoretic softness and xed charge density of the layer. Brush-coated glass could be distinguished from glass by a 2-3-fold decrease in xed charge density, while both surfaces were about equally soft. Adhesion of Staphylococcus epidermidis HBH276 to glass in a parallel plate ow chamber was extremely high and after 4 h, 19:0 £ 10 6 bacteria were adhering per cm 2 . In contrast, the organisms did not adhere to brush-coated glass, with numbers below the detection limit, i.e. 0:1 £ 10 6 per cm 2 . These results attest to the great potential of polymer brushes in preventing bacterial adhesion to surfaces.

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Grafting of High-Density Poly(Ethylene Glycol) Monolayers on Si(111)

Cited by 96 publications

References 20 publications

In Pursuit of Zero: Polymer Brushes that Resist the Adsorption of Proteins

In Pursuit of Zero: Polymer Brushes that Resist the Adsorption of Proteins

Thermocapillary actuation of droplets on chemically patterned surfaces by programmable microheater arrays

Characterization of poly(ethylene oxide) brushes on glass surfaces and adhesion of Staphylococcus epidermidis

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