Grafting of Fetal Thymus and Hematopoietic Tissue in Infants With Immune Deficiency Syndromes1

Githens, John H.; Fulginiti, Vincent A.; Suvatte, Vinai; Schröter, G; Hathaway, W. E.; Pearlman, David S.; Kay, H. E. M.; Terasaki, P. I.; Hill, George J.; Kempe, C. Henry; Cox, S.T.

doi:10.1097/00007890-197305000-00001

Cited by 30 publications

(6 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Cells obtained from each gradient layer were resuspended at a concentration of 3 X 106/ml in RPMI 1640 medium containing penicillin (50 units/ml) and streptomycin (50 ,ug/ml) and cocultured with thymic epithelium, thymic culture supernatant, or thymic extracts thymopoietin, and ubiquitin for 15 hr at 370 in a humidified 5% C02/95% air incubator. After incubation, cells were washed twice and their viability was assessed by trypan blue exclusion test.…”

Section: Methodsmentioning

confidence: 99%

“…Furthermore, at appropriate stages of development, fetal liver cells do not induce lethal graftversus-host disease (9)(10)(11)(12). Even though initial efforts to use fetal liver transplants to correct combined immunodeficiency were inconclusive, initiated graft-versus-host disease, or failed to achieve immunologic constitution (13)(14)(15), recently, transplantation of fetal liver was proved to be successful in achieving sustained immunological reconstitution in a limited number of patients with SCID for whom no histocompatible marrow donors were available (16)(17)(18)(19)(20). The In this report, we present evidence to indicate that, in certain variants of SCID, transplantation of both fetal liver and thymus may be necessary to provide both the stem cell and an inductive influence necessary for immunologic reconstitution.…”

mentioning

confidence: 99%

See 1 more Smart Citation

Rationale for combined use of fetal liver and thymus for immunological reconstitution in patients with variants of severe combined immunodeficiency

Pahwa

Good

et al. 1977

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

Bone marrow cells from a patient with severe combined immunodeficiency were studied in vitro for thymus-dependent lymphocyte (T cell) Severe combined immunodeficiency disease (SCID) is a diagnostic term for a heterogeneous group of congenital disorders usually ascribed to a defect in the development or function of lymphoid stem cells (1). Such patients usually have defective development and function of both thymus-dependent lymphocyte (T cell) and thymus-independent lymphocyte (B cell) populations (2). Immunologic reconstitution in patients with this disease has been repeatedly achieved after transplantation of marrow from related, histocompatible donors (3)(4)(5)(6)(7)(8) In this report, we present evidence to indicate that, in certain variants of SCID, transplantation of both fetal liver and thymus may be necessary to provide both the stem cell and an inductive influence necessary for immunologic reconstitution. MATERIALS AND METHODSCase Report. The patient, a 20-month-old white boy, was a full-term 3.8-kg product of a normal pregnancy. Growth and development were normal until age 10 weeks, at which time a monilial rash was first noted behind his right ear; this was followed by severe oral moniliasis at age 4 months. Both conditions were refractory to antifungal therapy. At 5 months of age, poor feeding and progressive weight loss developed. On Sept., 18, 1975, at age 7 months, he was admitted to our hospital.At the time of admission, the patient was markedly cachectic, weighed 5.5 kg, and had severe moniliasis involving the oral cavity and perineal area. Clinical and roentgenographic evidence of interstitial pneumonia, subsequently documented to be due to Pneumocystis carinii, was noted. No

show abstract

Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

Rationale for combined use of fetal liver and thymus for immunological reconstitution in patients with variants of severe combined immunodeficiency

Pahwa

Good

et al. 1977

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

show abstract

“…Subsequent studies have confirmed and extended this observation [34,58,237]. In humans, initial efforts to use frozen fetal liver transplants to correct combined immunodeficiency were inconclusive; this procedure either initiated GVHD and/or failed to achieve immunologic reconstitution [89,211]. Recently, transplantation of fresh fetal liver has been successfully used to achieve sustained immunologic reconstitution in a limited number of patients with SCID for whom no histocompatible marrow donors were available [1,45,127,169,174,191].…”

Section: B Transpandntation Of Fetal Tissues For Treatment Of Severe Cmentioning

confidence: 99%

Treatment of the immunodeficiency diseases — progress toward replacement therapy emphasizing cellular and macromolecular engineering

Pahwa

O’Reilly

et al. 1978

Springer Semin Immunopathol

View full text Add to dashboard Cite

An increasing challenge to physicians is represented by a sizable population of patients who suffer from immunodeficiency diseases. These diseases may involve deficiencies or imbalances of the specific immunologic defenses represented by the T-cell-mediated immunities, B-cell-mediated or humoral immunities, major biologic amplification systems of either cell-mediated or humoral immunities, and the fundamental effector mechanisms. The consequent extraordinary heterogeneity of these diseases implies that curative, corrective, or even replacement and supportive therapy, to be most effective, must be based on fundamental understanding of the nature of each disease, its specific diagnosis and analysis of its pathogenesis. Although much progress has been made toward understanding these diseases over the past 25 years, analyses are often incomplete, sometimes controversial, and occasionally entirely lacking, rendering therapeutic efforts less than ideal. Nonetheless, dramatic advances in therapy have been forthcoming. With rapidly improving understanding of the basic deficiencies underlying the diseases and with improvement in the therapeutic armamentarium, one of the most rewarding and indeed dramatic branches of medicine has been that derived from continuing efforts to treat patients with primary immunodeficiency diseases. It will be our purpose in this chapter to outline this rapidly developing field from a focus in our own experience with large numbers of patients having all currently recognized forms of primary immunodeficiency. It seems likely, from data and new therapeutic agents already at hand and under clinical and laboratory investigation, that the therapy of primary immunodeficiency will continue to develop rapidly in the years ahead and will surely be an important guide to improved treatment of secondary immunodeficiencies as well.

show abstract

“…A few practical considerations are suggested by this experience, especially in view of the fact that fetal liver transplantation has been attempted many times before in patients with CID without benefit (Hitzig et al 1965, Githens et al 1973. Most af the unsuccessful efforts have involved the use of frozen fetal liver cells.…”

Section: Fetal Liver Correction Of Cid In An a Da Deficient Infantmentioning

confidence: 99%

Developmental Defects of T and B Cell Lines in Humans

Cooper

Keightley

et al. 1973

Immunological Reviews

View full text Add to dashboard Cite

Grafting of Fetal Thymus and Hematopoietic Tissue in Infants With Immune Deficiency Syndromes1

Cited by 30 publications

References 0 publications

Rationale for combined use of fetal liver and thymus for immunological reconstitution in patients with variants of severe combined immunodeficiency

Rationale for combined use of fetal liver and thymus for immunological reconstitution in patients with variants of severe combined immunodeficiency

Treatment of the immunodeficiency diseases — progress toward replacement therapy emphasizing cellular and macromolecular engineering

Developmental Defects of T and B Cell Lines in Humans

Contact Info

Product

Resources

About