Grafting improves tomato yield under low nitrogen conditions by enhancing nitrogen metabolism in plants

Zhang, Zhi Huan; Li, Ming Ming; Cao, Bi Li; Chen, Zi Jing; Xu, Kun

doi:10.1007/s00709-021-01623-3

Cited by 22 publications

(12 citation statements)

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“…This nding suggests that the ue-cured tobacco rootstocks has an increased nutrient acquisition capacity, which was translated into higher leaf chlorophyll content and photosynthesis products [26][27] . These results are similar to those found in other eld crops and tomato, in which an increase of absorption and utilization of nitrogen and photosynthesis was observed in grafted plants [28,20 . In addition, studies have also shown that grafting signi cantly affected the content of endogenous hormones in plant leaves, thereby having regulatory effect on photosynthetic capacity of plant leaves [29][30] , which need to be veri ed in our study.…”

Section: Discussionsupporting

confidence: 91%

Reducing the nitrate content of burley tobacco by grafting with flue-cured tobacco

Feng

Zhao

et al. 2021

Preprint

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Background: Nitrosition of tobacco pyridine alkaloids by nitrate-derived NOx is the origin of tobacco-specifc nitrosamines (TSNAs) formed in tobacco, which are among the most notable toxicants present in tobacco products and smoke. Burley tobacco is particularly susceptible to TSNA formation because the cultivars exhibit a chloroplast-deficient and nitrogen-use-defciency phenotype which results in high accumulation of nitrate. Strategies to lower nitrate levels in tobacco could produce a corresponding decrease in TSNAs accumulation in leaves. Previous studies have showed that grafting with high nitrogen use efficiency rootstock was able to improve the nitrogen utilization of flue-cured tobacco. In this study, a reciprocativel grafting experiment has been conducted with two varieties of different tobacco types (burley tobacco ‘Eyan No.1’ and flue-cured tobacco ‘K326’) to investigate whether replacing burley tobacco root with flue-cured tobacco by grafting can enhance pigment biosynthesis and photosynthesis parameters and reduce nitrate content of burley tobacco leaves, and to explore the corresponding mechanism. Results: The results showed that compared with the self-rooted burley tobacco seedlings, grafting significantly increased the pigment content, net photosynthesis, biomass, total soluble sugar, reducing sugar, nitrate reductase activity, glutamine synthetase activities, NH4-N and soluble protein content of the leaves of Eyan No.1 while decreased the nitrate content and NO3-N/TN. Interestingly, transcription level analysis suggested that replacing burley tobacco root with flue-cured tobacco by grafting was able to up-regulate the genes involved in starch and sucrose metabolism, porphyrin and chlorophyll metabolism, carbon fixation in photosynthetic organism metabolism, carotenoid biosynthesis and nitrogen metabolism of burley tobacco leaves. In addition, the PPI network revealed gene_68511, gene_35043 and gene_77508 had higher degrees via replacing burley tobacco root with flue-cured tobacco, which might be hub proteins to reduce nitrate accumulation. Conclusions: In conclusion, grafting with high nitrogen use efficiency rootstock provided an exceptionally promising means of nitrate reduction in burley tobacco leaf , which is a principle precursor of TSNAs, due to the improvement of photosynthesis and nitrogen metabolism in the scion.

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Section: Discussionsupporting

confidence: 91%

Reducing the nitrate content of burley tobacco by grafting with flue-cured tobacco

Feng

Zhao

et al. 2021

Preprint

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“…8). Multiple experimental systems support this result, including those conducted in tomato 35 , cucumber 4 , and soybean 14 . GS and GOGAT were analyzed simultaneously with the amino acid synthesis process (Fig.…”

Section: Discussionmentioning

confidence: 61%

Reduced Application of Nitrogen Fertilizer Affects Nitrogen Metabolism of Leaves and Maintains the Production During the Harvesting Stage in Coreopsis tinctoria

Jiang

et al. 2022

Preprint

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Coreopsis tinctoria, as a medicinal plant, has become popular in China in the past decade. However, basic data on NO3− supply related to its production and nitrogen (N) metabolism are lacking. In this study, all plants were grown to four leaves in substrate (vermiculite:perlite = 2:1), which were subsequently transferred to different reducing NO3− solutions. Appropriate N fertilizer application reductions (1/8N-1/2N) maintained the flower number and biomass of Coreopsis tinctoria during 2020–2021. N fertilizer reduction maintained the N assimilation levels of C. tinctoria and increased N use efficiency (NUE), one of the important reasons was that the reduction of N fertilizer did not damage the chlorophyll fluorescence system and photosynthetic system. 1/2N-1/8N treatment also maintained the activities nitrate reductase (NR), nitrite reductase (NIR), glutamine synthase (GS) and glutamate synthase (GOGAT) and the content of total amino acid and soluble protein in leaves. N reduction treatment also increased the levels of tyrosine (Thr), serine (Ser), leucine (Leu), isoleucine (Ile), lysine (Lys) and arginine (Arg) of leaves in C. tinctoria, and further enhances N assimilation ability. Leaf N content was significantly correlated with the activities of NR, NIR, GS, GOGAT and GDH, indicating that N metabolism was effectively regulated by N levels. In conclusion, the 1/8 N level prior to harvest could be one of the strategies for C. tinctoria cultivation because it minimizes planting costs and maintains nitrogen assimilation capacity and productivity.

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“…Grafting over hardy rootstocks is an effective strategy for strengthening a plant’s resistance to climatic conditions [ 6 , 32 ]. LA1777 as a representative cold-tolerant germplasm from S. habrochaites might be utilized as rootstock to enhance the cold tolerance of tomato grafts.…”

Section: Discussionmentioning

confidence: 99%

The essential role of jasmonate signaling in Solanum habrochaites rootstock-mediated cold tolerance in tomato grafts

Wang

Chen

et al. 2022

Horticulture Research

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Tomato (Solanum lycopersicum) is among the most important vegetables across the world, but cold stress usually affects its yield and quality. The wild tomato species Solanum habrochaites is commonly utilized as rootstock for enhancing the resistance against abiotic stresses in cultivated tomato, especially cold resistance. However, the underlying molecular mechanism remains unclear. In this research, we confirmed that S. habrochaites rootstock can improve the cold tolerance of cultivated tomato scions, as is revealed by growth, physiological and biochemical indicators. Furthermore, transcriptome profiling indicated significant differences in the scion of homo- and hetero-grafted seedlings, including substantial changes in jasmonic acid biosynthesis and signaling, which were validated by RT-qPCR analysis. S. habrochaites plants had a high basal level of jasmonate, and cold stress caused a greater amount of active JA-Ile in the S. habrochaites hetero-grafts. Moreover, exogenous JA enhanced while JA inhibitor decreased the cold tolerance of tomato grafts. The JA biosynthesis defected mutant (spr8) also showed increased sensitivity to cold stress. All of these results demonstrated the significance of JA in the cold tolerance of grafted tomato seedlings with S. habrochaites rootstock, suggesting a future direction on characterization of natural variation involved in S. habrochaites rootstock-mediated cold tolerance.

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Grafting improves tomato yield under low nitrogen conditions by enhancing nitrogen metabolism in plants

Cited by 22 publications

References 50 publications

Reducing the nitrate content of burley tobacco by grafting with flue-cured tobacco

Reducing the nitrate content of burley tobacco by grafting with flue-cured tobacco

Reduced Application of Nitrogen Fertilizer Affects Nitrogen Metabolism of Leaves and Maintains the Production During the Harvesting Stage in Coreopsis tinctoria

The essential role of jasmonate signaling in Solanum habrochaites rootstock-mediated cold tolerance in tomato grafts

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