Grafting Hyaluronic Acid onto Gold Surface to Achieve Low Protein Fouling in Surface Plasmon Resonance Biosensors

Liu, Xia; Huang, Renliang; Su, Rongxin; Qi, Wei; Wang, Libing; He, Zhimin

doi:10.1021/am502921z

Cited by 117 publications

(105 citation statements)

References 51 publications

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“…36), has also been recently developed (in 2014). The level of fouling from cow milk (Γ = 9.8 ng cm −2 -10 min exposure) 236 was comparable to that reported in the aforementioned studies with polymer brushes and OEG n -SAMs (vide supra). The level of fouling from cow milk (Γ = 9.8 ng cm −2 -10 min exposure) 236 was comparable to that reported in the aforementioned studies with polymer brushes and OEG n -SAMs (vide supra).…”

Section: Iiid Milksupporting

confidence: 84%

A survey of state-of-the-art surface chemistries to minimize fouling from human and animal biofluids

2015

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Upon contact with bodily fluids, synthetic materials spontaneously acquire a layer of various species (most notably proteins) on their surface. The concern with respect to biomedical equipment, implants or devices resides in the possibility for biological processes with potentially harmful effects to ensue. In biosensor technology, the issue with this natural fouling phenomenon is that of non-specific adsorption to sensing platforms, which generates an often overwhelming interference signal that prevents the detection, not to mention the quantification, of target analytes present at considerably lower concentration. To alleviate this ubiquitous, recurrent problem - this genuine biotechnological plague - considerable research efforts have been devoted over the last few decades to engineer antifouling coatings. Extensive literature now exists that describes stealth organic adlayers capable of reducing fouling surface coverage Γ down to a few ng cm(-2)- however from biotechnologically irrelevant buffered solutions free or nearly depleted of any potentially interfering species. Regrettably indeed, few coatings are known to display/retain such level of performance when exposed to otherwise more complex, real-life biosamples (even diluted). Herein, we comprehensively review the state-of-the-art surface chemistries developed to date (January 2015) to minimize fouling from 8 such uncomparatively more challenging biological media (blood plasma, blood serum, cell lysate, cerebrospinal fluid, egg, milk, saliva, and urine) - whether of human or animal origin. Literature search for another 25 biological milieux generated no (exploitable) hit. Also discussed in this Review are the identification of the species responsible for fouling, and the dependence of antifouling properties on biosample source variability.

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Section: Iiid Milksupporting

confidence: 84%

A survey of state-of-the-art surface chemistries to minimize fouling from human and animal biofluids

2015

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“…213 Furthermore, a bioorthogonal handle, such as an azide and an alkyne, was chemically introduced into polysaccharides for bioorthogonal conjugation chemistry, such as CuAAC and Staudinger reactions. 221 In order to efficiently reduce the inflammation response induced by TNF-α, HAs were conjugated to anti-TNF-α antibodies through reactions between carboxyl acids of the HAs and amines of the antibodies. 216 Although we briefly summarized the recent progress on hyaluronic acid-polymer conjugation here, it should be noted that bioorthogonal chemistries were rarely used.…”

Section: Polysaccharidementioning

confidence: 99%

Expansion of bioorthogonal chemistries towards site-specific polymer–protein conjugation

Jung

Kwon

2016

Polym. Chem.

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Polymer conjugation to proteins has been widely used to improve or expand protein properties. However, polymer conjugation to random sites of a target protein often led to a significant loss of critical protein properties. In order to overcome this, polymer conjugation to specific sites of a protein was developed using the site-specific introduction of non-natural amino acids and bioorthogonal chemistries. This review summarizes the recent advances in bioorthogonal chemistries. As the repertoire of bioorthogonal chemistries available for polymer conjugation is expanding, the site-specific polymer conjugation technique would be a valuable platform technique to design novel protein-polymer conjugates.

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“…10,11,[26][27][28][29] PEI is the branched polymer with high molecular weight. 10,11,[26][27][28][29] PEI is the branched polymer with high molecular weight.…”

Section: Characterization Of Zwitterionic Peimentioning

confidence: 99%

Constructing a zwitterionic ultrafiltration membrane surface via multisite anchorage for superior long-term antifouling properties

Zhu

Zhao

et al. 2015

RSC Adv.

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Zwitterions bearing balanced charge groups have attracted increasing attention to fabricate antifouling membranes due to their highly hydrated structure. The simple and efficient method of covalent connection to enhance the stability of the zwitterions on membrane surfaces is still challenging. Herein, s branched polyethyleneimine (PEI) was employed to synthesize the zwitterion by a quaternary amination reaction with sodium chloroacetate. A novel zwitterionic surface with neutral surface charge was constructed by grafting the PEI-based zwitterion (Z-PEI) onto the hydrolyzed PAN (H-PAN) membrane surface. Covalent bonds were formed between the Z-PEI and H-PAN membrane while the electrostatic attraction would promote this reaction. The zwitterionic membranes exhibited superior antifouling ability (flux recovery ratio of about 99.8% and total flux decline of about 31.4%) due to the formation of a tight hydration layer by zwitterions on the membrane surface. Furthermore, the flux recovery ratio was not changed obviously during the long term experiment and could be maintained at as high as 96.3 and 98.4% after immersion in acid and alkali solution, respectively. These results demonstrated that the long term and chemical stability of zwitterionic functional surfaces were significantly enhanced via multisite covalent anchorage.

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Grafting Hyaluronic Acid onto Gold Surface to Achieve Low Protein Fouling in Surface Plasmon Resonance Biosensors

Cited by 117 publications

References 51 publications

A survey of state-of-the-art surface chemistries to minimize fouling from human and animal biofluids

A survey of state-of-the-art surface chemistries to minimize fouling from human and animal biofluids

Expansion of bioorthogonal chemistries towards site-specific polymer–protein conjugation

Constructing a zwitterionic ultrafiltration membrane surface via multisite anchorage for superior long-term antifouling properties

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