Grafting Compatibility, Scion Growth, and Fusarium Wilt Disease Incidence of Intraspecific Grafted Tomato

Abstract

The tomato (Solanum lycopersicum L.) is one of the most important vegetables grown globally. However, the production of tomatoes is restricted by Fusarium oxysporum f. sp. lycopersici (Fol). This study aims to investigate the ability of Fol-resistant tomato genotypes to be a rootstock for the susceptible cultivar. In this study, a tomato cultivar was grafted on rootstocks of the same species (intraspecific), and grafting compatibility, peroxidase gene expression, and fusarium wilt disease incidence of tomato scion was evaluated. A Fol-susceptible tomato ‘Sidathip 3’ (SDT3) was grafted onto four different Fol-resistant tomato genotypes and compared with self-grafted cultivar/cultivar and rootstock/rootstock. The survival rate of all grafted plants was 100% at 20 days after grafting (DAG) without significant differences in incompatibility evaluated at 42 days after grafting. The expression of the peroxidase gene (Solyc02g084800.2) using the qPCR technique was compared in self-grafted rootstock LE472/LE472 and SDT3/LE472. The expression level was three times higher in heterografted plants than in self-grafted ones at 15 DAG, indicating graft incompatibility. The rootstocks did not affect the height of the plant, the number of branches, the size of the fruit, or the yield of SDT3 scion. All intraspecific heterografted plants significantly controlled Fol when evaluated 60 days after inoculation. These results showed the usefulness of intraspecific grafting by using the proper rootstock genotypes to increase pathogen resistance in addition to stimulating growth and fruit yield.

Evaluation and Genome Mining of Bacillus stercoris Isolate B.PNR1 as Potential Agent for Fusarium Wilt Control and Growth Promotion of Tomato

“…The isolate TFPK401 of Fol race 1, which is a reference isolate for Fol race 1-resistant tomato screening ( Kawicha et al, 2023b ) and disease control assessments ( Kawicha et al, 2023a ; Saman et al, 2022 ) and nine other isolates of Fol were used to evaluate the antagonistic efficacy of the isolated Bacillus . The other fungal pathogens, including 10 isolates of F. oxysporum f. sp.…”

Genetic loci associated with Fusarium wilt resistance in tomato (Solanum lycopersicum L.) discovered by genome‐wide association study

“…lycopersici ( Fol ) is an important causal agent causing tomato losses in the fields and greenhouses (McGovern, 2015). The Fol pathogen has entered the tomato roots by direct penetration or through wounds and invaded to colonise the roots and stems xylem vessels (Saman et al, 2022; Srinivas et al, 2019), resulting in tomato wilt symptoms. Fol is challenging to remove because it can persist in soil for up to 10 years as thick‐walled chlamydospores (Jones et al, 2014).…”

“…fields and greenhouses (McGovern, 2015). The Fol pathogen has entered the tomato roots by direct penetration or through wounds and invaded to colonise the roots and stems xylem vessels (Saman et al, 2022;Srinivas et al, 2019), resulting in tomato wilt symptoms.…”