Grafted skeletal myoblast sheets attenuate myocardial remodeling in pacing-induced canine heart failure model

Abstract

Grafting of skeletal myoblast sheets attenuated cardiac remodeling and improved cardiac performance. This novel method was feasible and effective in a large animal model, suggesting an innovative and promising strategy for treating patients with end-stage dilated cardiomyopathy.

Concise Review: Cell Therapy and Tissue Engineering for Cardiovascular Disease

“…Kondoh et al showed that the transplantation of skeletal myoblast sheets improved a cardiac performance and prolonged the life span of animals, associating with the reorganization of the cytoskeletal proteins of host cardiac tissue and the reduction of myocardial fibrosis, using a dilated cardiomyopathy hamster model [71]. The significant therapeutic effects by the transplantation of autologous skeletal myoblast sheets were also confirmed in large-animal modes (a pacing-induced canine dilated cardiomyopathy heart failure model and a porcine ischemic myocardium model) [72,73]. The transplantations of several adult stem cell sheets (adipose-derived and menstrual blood-derived mesenchymal stem cell sheets, and CSC sheets) also gave favorable therapeutic effects in small-animal models [41,74,75].…”

Cardiac Differentiation of Embryonic Stem Cells by Patterning Culture

“…As mentioned above, scaffold-free engineered tissues are favorable to be transplanted for myocardial regeneration. We have demonstrated the therapeutic effectiveness of the cell sheets created from cardiomyocytes , myoblasts (Hata et al, 2006;Kondoh et al, 2006;Memon et al, 2005), and cardiac progenitor cells (Matsuura et al, 2009). We also started the clinical study of myoblast-sheet transplantation for DCM patients.…”

“…As the alternative to heart transplantation, regenerative medicine based on cell transplantation attracts increasing attention. Recent studies revealed that bone marrow-derived cells (Messina et al, 2004;Wollert et al, 2004), endothelial progenitor cells (Kawamoto et al, 2001), adipose-derived cells (Wang et al, 2009), and myoblasts Hata et al, 2006;Kondoh et al, 2006) have the potential to improve cardiac function when they are transplanted into a failing heart. Because these somatic cells can be harvested from the patients themselves, their clinical applications have already started.…”

Cell Sheet Tissue Engineering for Heart Failure

“…In a pacing-induced canine dilated cardiomyopathy model, transplantation of skeletal myoblast grafts has shown improved cardiac function with reduction of fibrosis and apoptosis [17]. In a porcine cardiac infarction model, skeletal myoblast graft transplantation provided improvement of cardiac function with attenuation of cardiac remodeling [18].…”