Grafted Activated Schwann Cells Support Survival of Injured Rat Spinal Cord White Matter

Marcol, Wiesław; Ślusarczyk, Wojciech; Larysz‐Brysz, Magdalena; Francuz, Tomasz; Jędrzejowska–Szypułka, Halina; Łabuzek, Krzysztof; Lewin–Kowalik, Joanna

doi:10.1016/j.wneu.2015.04.027

Cited by 5 publications

(5 citation statements)

References 52 publications

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“…SCs secrete ECM components (Tabesh et al, 2009 ) and neurotrophic factors such as brain-derived neurotrophic factor (BDNF), neurotrophin-3 (NT-3), nerve growth factor (NGF) (Dey et al, 2013 ; Godinho et al, 2013 ) and glial cell-derived neurotrophic factor (GDNF) (Iannotti et al, 2004 ). Therefore, transplantation of SCs after SCI has been widely studied and numerous results demonstrate that SCs can support survival and maintenance of spared neural tissue, bridge lesion cavities, promote re-growth of both motor and sensory axons into the lesion after engraftment and remyelinate CNS axons (Xu et al, 1997 ; Weidner et al, 1999 ; Marcol et al, 2015 ). Unfortunately, SCs form a distinct divide between themselves and CNS tissue both in vitro and in vivo (Ghirnikar and Eng, 1994 ; Lakatos et al, 2000 ).…”

Section: Cellular Candidatesmentioning

confidence: 99%

Biomaterial-Supported Cell Transplantation Treatments for Spinal Cord Injury: Challenges and Perspectives

Liu

Schackel

Weidner

et al. 2018

Front. Cell. Neurosci.

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Spinal cord injury (SCI), resulting in para- and tetraplegia caused by the partial or complete disruption of descending motor and ascending sensory neurons, represents a complex neurological condition that remains incurable. Following SCI, numerous obstacles comprising of the loss of neural tissue (neurons, astrocytes, and oligodendrocytes), formation of a cavity, inflammation, loss of neuronal circuitry and function must be overcome. Given the multifaceted primary and secondary injury events that occur with SCI treatment options are likely to require combinatorial therapies. While several methods have been explored, only the intersection of two, cell transplantation and biomaterial implantation, will be addressed in detail here. Owing to the constant advance of cell culture technologies, cell-based transplantation has come to the forefront of SCI treatment in order to replace/protect damaged tissue and provide physical as well as trophic support for axonal regrowth. Biomaterial scaffolds provide cells with a protected environment from the surrounding lesion, in addition to bridging extensive damage and providing physical and directional support for axonal regrowth. Moreover, in this combinatorial approach cell transplantation improves scaffold integration and therefore regenerative growth potential. Here, we review the advances in combinatorial therapies of Schwann cells (SCs), astrocytes, olfactory ensheathing cells (OECs), mesenchymal stem cells, as well as neural stem and progenitor cells (NSPCs) with various biomaterial scaffolds.

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Section: Cellular Candidatesmentioning

confidence: 99%

Biomaterial-Supported Cell Transplantation Treatments for Spinal Cord Injury: Challenges and Perspectives

Liu

Schackel

Weidner

et al. 2018

Front. Cell. Neurosci.

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“…24 In addition, the neurotrophic factors and cell adhesion molecules secreted by ASCs can also induce axon myelination and neuronal survival. 25 While several studies have shown that single SC repair of SCI is still limited, researchers try to transplant it with other stem cells in order to achieve better effects. [26][27][28] Coculture of SCs with neural stem cells (NSCs) can remarkably increase the proportion of neuron differentiation and effectively improve the recovery of nerve endings.…”

Section: Introductionmentioning

confidence: 99%

Polycaprolactone electrospun fiber scaffold loaded with iPSCs-NSCs and ASCs as a novel tissue engineering scaffold for the treatment of spinal cord injury

Zhou¹,

Shi²,

Fan³

et al. 2018

IJN

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BackgroundSpinal cord injury (SCI) is a traumatic disease of the central nervous system, accompanied with high incidence and high disability rate. Tissue engineering scaffold can be used as therapeutic systems to provide effective repair for SCI.PurposeIn this study, a novel tissue engineering scaffold has been synthesized in order to explore the effect of nerve repair on SCI.Patients and methodsPolycaprolactone (PCL) scaffolds loaded with actived Schwann cells (ASCs) and induced pluripotent stem cells -derived neural stem cells (iPSC-NSCs), a combined cell transplantation strategy, were prepared and characterized. The cell-loaded PCL scaffolds were further utilized for the treatment of SCI in vivo. Histological observation, behavioral evaluation, Western-blot and qRT-PCR were used to investigate the nerve repair of Wistar rats after scaffold transplantation.ResultsThe iPSCs displayed similar characteristics to embryonic stem cells and were efficiently differentiated into neural stem cells in vitro. The obtained PCL scaffolds werê0.5 mm in thickness with biocompatibility and biodegradability. SEM results indicated that the ASCs and (or) iPS-NSCs grew well on PCL scaffolds. Moreover, transplantation reduced the volume of lesion cavity and improved locomotor recovery of rats. In addition, the degree of spinal cord recovery and remodeling maybe closely related to nerve growth factor and glial cell-derived neurotrophic factor. In summary, our results demonstrated that tissue engineering scaffold treatment could increase tissue remodeling and could promote motor function recovery in a transection SCI model.ConclusionThis study provides preliminary evidence for using tissue engineering scaffold as a clinically viable treatment for SCI in the future.

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“…Compared with NSCs, ASCs have a higher cell proliferative and adhesive ability, and can synthesize and secrete various neurotrophic factors (25). Marcol et al (26) used ASCs to repair focal injury in the spinal cords of rats. In particular, they found the transplanted ASCs could elicit the body's self-repair by inducing endogenous SCs in the nerve root to migrate to the lesion site (26).…”

Section: Discussionmentioning

confidence: 99%

“…Marcol et al (26) used ASCs to repair focal injury in the spinal cords of rats. In particular, they found the transplanted ASCs could elicit the body's self-repair by inducing endogenous SCs in the nerve root to migrate to the lesion site (26). Moreover, a previous study by the authors demonstrated that the clinical application of ASCs in the treatment of patients with SCI exhibited promising prospects (15).…”

Section: Discussionmentioning

confidence: 99%

Gelatin methacryloyl hydrogel scaffold loaded with activated Schwann cells attenuates apoptosis and promotes functional recovery following spinal cord injury

Liu

Hao

et al. 2023

Exp Ther Med

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Spinal cord injury (SCI) is a refractory disease of the central nervous system with a high disability and incidence rate. In recent years, bioactive material combined with cell transplantation has been considered an effective method for the treatment of SCI. The present study encapsulated activated Schwann cells (ASCs) in a 3D gelatin methacryloyl (GelMA) hydrogel in order to investigate its therapeutic effects on SCI. ASCs were isolated from previously ligated rat sciatic nerves. Scanning electron microscopy and live/dead staining were used to evaluate the biocompatibility of hydrogels with the ASCs. The scaffold was transplanted into the spinal cord of rats in the hemisection model. Behavioral tests and hematoxylin and eosin staining were employed to assess the locomotion recovery and lesion areas before and after treatment. Cell apoptosis was evaluated using TUNEL staining and immunochemistry, and apoptosis-related protein expression was detected using western blot analysis. The ASCs exhibited a favorable survival and proliferative ability in the 3D GelMA hydrogel. The scaffold transplantation significantly reduced the cavities and improved functional recovery. Moreover, the GelMA/ASCs implants significantly inhibited cell apoptosis following SCI and this effect may be mediated via the p38 MAPK pathway. Overall, these findings indicated that ASCs combined with the 3D GelMA hydrogel may be a promising therapeutic strategy for SCI.

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Grafted Activated Schwann Cells Support Survival of Injured Rat Spinal Cord White Matter

Cited by 5 publications

References 52 publications

Biomaterial-Supported Cell Transplantation Treatments for Spinal Cord Injury: Challenges and Perspectives

Biomaterial-Supported Cell Transplantation Treatments for Spinal Cord Injury: Challenges and Perspectives

Polycaprolactone electrospun fiber scaffold loaded with iPSCs-NSCs and ASCs as a novel tissue engineering scaffold for the treatment of spinal cord injury

Gelatin methacryloyl hydrogel scaffold loaded with activated Schwann cells attenuates apoptosis and promotes functional recovery following spinal cord injury

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