Graft-related endocarditis caused by Neosartorya fischeri var. spinosa

Summerbell, Richard C.; Repentigny, Louis de; Chartrand, C; Germain, G St

doi:10.1128/jcm.30.6.1580-1582.1992

Cited by 32 publications

(12 citation statements)

References 7 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…As a large number of new species involved in IA have been recently reported, Aspergillus species identification has become a real challenge in clinical laboratories. Indeed, a major shift has been observed in the recent epidemiology of [14], N. pseudofischeri [15], N. hiratsukae [16], A. calidoustus [19] and A. niveus [20]. These species, usually found in the environment (i.e.…”

Section: Discussionmentioning

confidence: 99%

“…Two distinct phenomena have been observed. First, recent studies have documented cases of IA caused by new Aspergillus species, such as N. fischeri [14], N. pseudofischeri [15], N. hiratsukae [16], A. calidoustus [19] and A. niveus [20]. These species, usually found in the environment (i.e.…”

Section: Discussionmentioning

confidence: 99%

“…Some of the newly described species responsible for IA have decreased susceptibilities to multiple antifungal drugs in vitro [8,10–13], and therapeutic failures have been reported in cases of infection with Neosartorya fischeri [14], Neosartorya pseudofischeri [15], Neosartorya hiratsukae [16], Neosartorya udagawae [17], Aspergillus insuetus [18], Aspergillus calidoustus [19] and Aspergillus niveus [20]. These species are difficult to separate from more susceptible Aspergillus species, sometimes from the same section, solely on the basis of morphological criteria.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Matrix‐assisted laser desorption ionization time‐of‐flight mass spectrometry for fast and accurate identification of clinically relevant Aspergillus species

Alanio

Béretti

Dauphin

et al. 2011

Clinical Microbiology and Infection

154

112

View full text Add to dashboard Cite

New Aspergillus species have recently been described with the use of multilocus sequencing in refractory cases of invasive aspergillosis. The classical phenotypic identification methods routinely used in clinical laboratories failed to identify them adequately. Some of these Aspergillus species have specific patterns of susceptibility to antifungal agents, and misidentification may lead to inappropriate therapy. We developed a matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) mass spectrometry (MS)-based strategy to adequately identify Aspergillus species to the species level. A database including the reference spectra of 28 clinically relevant species from seven Aspergillus sections (five common and 23 unusual species) was engineered. The profiles of young and mature colonies were analysed for each reference strain, and species-specific spectral fingerprints were identified. The performance of the database was then tested on 124 clinical and 16 environmental isolates previously characterized by partial sequencing of the β-tubulin and calmodulin genes. One hundred and thirty-eight isolates of 140 (98.6%) were correctly identified. Two atypical isolates could not be identified, but no isolate was misidentified (specificity: 100%). The database, including species-specific spectral fingerprints of young and mature colonies of the reference strains, allowed identification regardless of the maturity of the clinical isolate. These results indicate that MALDI-TOF MS is a powerful tool for rapid and accurate identification of both common and unusual species of Aspergillus. It can give better results than morphological identification in clinical laboratories.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Matrix‐assisted laser desorption ionization time‐of‐flight mass spectrometry for fast and accurate identification of clinically relevant Aspergillus species

Alanio

Béretti

Dauphin

et al. 2011

Clinical Microbiology and Infection

154

112

View full text Add to dashboard Cite

show abstract

“…A. fischeri and A. thermomutatus have been identified as the cause of human mycoses in only few case reports with a broad spectrum of disease’s presentation including extrapulmonary manifestations in most cases, such as endocarditis, osteomyelitis, peritonitis, gastro-intestinal tract infection, keratitis, and disseminated infections ( Gerber et al, 1973 ; Coriglione et al, 1990 ; Summerbell et al, 1992 ; Padhye et al, 1994 ; Lonial et al, 1997 ; Chim et al, 1998 ; Gori et al, 1998 ; Matsumoto et al, 2002 ; Jarv et al, 2004 ; Balajee et al, 2005b ; Ghebremedhin et al, 2009 ; Toskova et al, 2013 ; Khare et al, 2014 ). Susceptibility testing of these isolates usually shows higher MICs for triazoles compared to A. fumigatus , but similar values for amphotericin B and echinocandins ( Balajee et al, 2005b ; Alcazar-Fuoli et al, 2008 ).…”

Section: Aspergillus ( Neosartorya ) mentioning

confidence: 99%

Aspergillus fumigatus-Related Species in Clinical Practice

2016

View full text Add to dashboard Cite

Aspergillus fumigatus is the main etiologic agent of invasive aspergillosis (IA). Other Aspergillus species belonging to the section Fumigati (A. fumigatus complex) may occasionally be the cause of IA. These strains are often misidentified, as they cannot be distinguished from A. fumigatus by conventional morphological analysis and sequencing methods. This lack of recognition may have important consequences as these A. fumigatus-related species often display some level of intrinsic resistance to azoles and other antifungal drugs. A. lentulus, A. udagawae, A. viridinutans, and A. thermomutatus (Neosartorya pseudofischeri) have been associated with refractory cases of IA. Microbiologists should be able to suspect the presence of these cryptic species behind a putative A. fumigatus isolate on the basis of some simple characteristics, such as defect in sporulation and/or unusual antifungal susceptibility profile. However, definitive species identification requires specific sequencing analyses of the beta-tubulin or calmodulin genes, which are not available in most laboratories. Multiplex PCR assays or matrix-assisted laser desorption ionization – time-of-flight mass spectrometry (MALDI-TOF MS) gave promising results for rapid and accurate distinction between A. fumigatus and other Aspergillus spp. of the section Fumigati in clinical practice. Improved diagnostic procedures and antifungal susceptibility testing may be helpful for the early detection and management of these particular IA cases.

show abstract

“…fischeri, reported by Coriglione et al (2) (CDC B-5491 = NRRL 22808), and N. fischeri var. spinosa, reported by Summerbell et al (15) (UAMH 7109 = CDC B-5472 = NRRL A-28710), from clinical cases of aspergillosis were obtained for taxonomic reexamination.…”

Section: Pseudofischerimentioning

confidence: 99%

Osteomyelitis caused by Neosartorya pseudofischeri

et al. 1994

View full text Add to dashboard Cite

The first case of osteomyelitis caused by Neosartorya pseudofischeri is reported. The patient, a 77-year-old male with a history of silicosis and tuberculosis, on X-ray examination revealed lytic lesions of L2 and L3 vertebrae suspicious for metastatic lesions. Histologic examination of biopsy specimens from vertebral bodies showed short, distorted, extra- and intracellular, hyaline hyphal fragments. The culture from the biopsy tissue produced numerous, evanescent asci containing eight ellipsoidal ascospores with two distinctive equatorial bands ca. 1 micron wide. When examined by a scanning electron microscope, ascospores exhibited a convex surface ornamented with raised flaps of tissue, in shape resembling triangular projections or long ridge lines. The conidial state (anamorph) was identified as Aspergillus thermomutatus on the basis of conidial columns which were smaller and less tightly packed as well as of a lighter shade of green than those observed in Aspergillus fumigatus. On the basis of the morphologic features of the ascospores, the teleomorph was identified as N. pseudofischeri.

show abstract

Graft-related endocarditis caused by Neosartorya fischeri var. spinosa

Cited by 32 publications

References 7 publications

Matrix‐assisted laser desorption ionization time‐of‐flight mass spectrometry for fast and accurate identification of clinically relevant Aspergillus species

Matrix‐assisted laser desorption ionization time‐of‐flight mass spectrometry for fast and accurate identification of clinically relevant Aspergillus species

Aspergillus fumigatus-Related Species in Clinical Practice

Osteomyelitis caused by Neosartorya pseudofischeri

Contact Info

Product

Resources

About