Graft photopolymerization of polyethylene glycol monoacrylate (PEGA) on poly(methyl methacrylate) (PMMA) films to prevent BSA adsorption

Iguerb, Ourida; Bertrand, Patrick

doi:10.1002/sia.2701

Cited by 23 publications

(28 citation statements)

References 19 publications

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“…Although a few studies have been reported by several authors [26][27][28], this technique will not be detailed herein. low practical value (Scheme 13.8).…”

Section: Photoinduced Grafting Methodsmentioning

confidence: 99%

Photografting of Polymeric Materials

Müftüoğlu

Taşdelen

Yaǧcı

2010

Photochemistry and Photophysics of Polymer Materials

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“…Although a few studies have been reported by several authors [26][27][28], this technique will not be detailed herein. low practical value (Scheme 13.8).…”

Section: Photoinduced Grafting Methodsmentioning

confidence: 99%

Photografting of Polymeric Materials

Müftüoğlu

Taşdelen

Yaǧcı

2010

Photochemistry and Photophysics of Polymer Materials

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“…PEG has been grafted onto biomaterial surfaces to provide a biocompatibility layer that reduces the absorption of plasma albumen and red blood cells [25,26]. Iguerb et al [27] prepared antifouling surfaces by graft polymerization of polyethylene glycol monoacrylate (PEGA) under UV irradiation. Benzophenone is used as photosensitizer to generate polymeric radicals on the surface of the poly (methyl methacrylate) (PMMA) film.…”

Section: Bioinert Surface Of Biomaterialsmentioning

confidence: 99%

Surface modification of biomaterials by photochemical immobilization and photograft polymerization to improve hemocompatibility

Feng

Zhao

Zhang

et al. 2010

Front. Chem. Eng. China

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Thrombus formation and blood coagulation are serious problems associated with blood contacting products, such as catheters, vascular grafts, artificial hearts, and heart valves. Recent progresses and strategies to improve the hemocompatibility of biomaterials by surface modification using photochemical immobilization and photograft polymerization are reviewed in this paper. Three approaches to modify biomaterial surfaces for improving the hemocompatibility, i.e., bioinert surfaces, immobilization of anticoagulative substances and biomimetic surfaces, are introduced. The biomimetic amphiphilic phosphorylcholine and Arg-Gly-Asp (RGD) sequence are the most effective and most often employed biomolecules and peptide sequence for improving hemocompatibility of material surfaces. The RGD sequence can enhance adhesion and growth of endothelial cells (ECs) on material surfaces and increase the retention of ECs under flow shear stress conditions. This surface modification is a promising strategy for biomaterials especially for cardiovascular grafts and functional tissue engineered blood vessels.

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“…ESI-5, † total (−)), were used to normalize (for each pixel) the intensities of DNA-derived negative ions and to yield the chemical maps of oligonucleotide composition within a single spot (Fig. 9) of streptavidin (signal C 9 H 8 N + , characteristic for tryptophan), 36,37 BSA (signal C 5 H 10 N + specific for lysine), 36,38,39 epoxy-terminated silane GOPS (signal CH 3 O + ) 23 and silicon substrate (signal Si + ). Moreover, the maps of total positive intensities, pointing to material distribution (Fig.…”

Section: Detection Of Pcr Products With Dna Arrays Prepared Accordingmentioning

confidence: 99%

Imaging and spectroscopic comparison of multi-step methods to form DNA arrays based on the biotin–streptavidin system

Gajos

Petrou²,

Budkowski

et al. 2015

Analyst

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Three multi-step multi-molecular approaches using the biotin-streptavidin system to contact-print DNA arrays on SiO2 surfaces modified with (3-glycidoxypropyl)trimethoxysilane are examined after each deposition/reaction step by atomic force microscopy, X-ray photoelectron spectroscopy and time of flight secondary ion mass spectrometry. Surface modification involves the spotting of preformed conjugates of biotinylated oligonucleotides with streptavidin onto surfaces coated with biotinylated bovine serum albumin b-BSA (approach I) or the spotting of biotinylated oligonucleotides onto a streptavidin coating, the latter prepared through a reaction with immobilized b-BSA (approach II) or direct adsorption (approach III). AFM micrographs, quantified by autocorrelation and height histogram parameters (e.g. roughness), reveal uniform coverage after each modification step with distinct nanostructures after the reaction of biotinylated BSA with streptavidin or of a streptavidin conjugate with biotinylated oligonucleotides. XPS relates the immobilization of biomolecules with covalent binding to the epoxy-silanized surface. Protein coverage, estimated from photoelectron attenuation, shows that regarding streptavidin the highest and the lowest immobilization efficiency is achieved by following approaches I and III, respectively, as confirmed by TOF-SIMS microanalysis. The size of the DNA spot reflects the contact radius of the printed droplet and increases with protein coverage (and roughness) prior to the spotting, as epoxy-silanized surfaces are hardly hydrophilic. Representative TOF-SIMS images show sub-millimeter spots: uniform for approach I, doughnut-like (with a small non-zero minimum) for approach II, both with coffee-rings or peak-shaped for approach III. Spot features, originating from pinned contact lines and DNA surface binding and revealed by complementary molecular distributions (all material, DNA, streptavidin, BSA, epoxy, SiO2), indicate two modes of droplet evaporation depending on the details of each applied approach.

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Graft photopolymerization of polyethylene glycol monoacrylate (PEGA) on poly(methyl methacrylate) (PMMA) films to prevent BSA adsorption

Cited by 23 publications

References 19 publications

Photografting of Polymeric Materials

Photografting of Polymeric Materials

Surface modification of biomaterials by photochemical immobilization and photograft polymerization to improve hemocompatibility

Imaging and spectroscopic comparison of multi-step methods to form DNA arrays based on the biotin–streptavidin system

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