Gradient elution of organic acids on a β-cyclodextrin column in the polar organic mode and its application to drug discovery

Emerging Analytical Separation Techniques with High Throughput Potential for Pharmaceutical Analysis, Part II: Novel Chromatographic Modes

“…POSC methods were developed to be applied in drug discovery in several publications [46][47][48][49]. The finally chosen experimental conditions for these applications are presented in Table 5.…”

“…In [46], cinnamic acid derivatives were used to develop a set of POSC-DAD or POSC-DAD/MS conditions to separate potential drug candidates (organic acids). The applied SP (Cyclobond I 2000 -cyclodextrin) had a -cyclodextrin as chiral selector.…”

Supramolecular Chiro-Biomedical Assays and Enantioselective HPLC Analyses for Evaluation of Profens as Non-Steroidal Anti-Inflammatory Drugs, Potential Anticancer Agents and Common Xenobiotics

“…Moreover, gradient elution increases throughput of analyses of large number of synthesized new drug-like racemates and enables full compatibility with commonly used mass spectrometry detection [10,11]. In order to understand which CSPs and HPLC conditions give benefits to various pharmaceutical requirements, and to identify the set of valuable alternative chiral separation procedures for resolving a given racemate, Borman et al [10] reported results of systematic screening of the 66 chiral HPLC systems for over 100 diverse racemic drugs which were operated in gradient normal phase, polar organic and aqueous modes with UV detection.…”

“…The use of small synthetic receptors, host-guest systems, molecular cages, ionophores, synthetic and chemically modified natural supramolecular polymers gives a broad range of currently available construction approaches for the induction, transcription, detection, amplification, monitoring, control and application of noncovalent bonding interaction with superior cooperativity in various supramolecular chirality sensing modes, including HPLC systems [1,[5][6][7]. However, the standardization of available protocols linked with chiral separation development offered by liquid and supercritical fluid chromatography to enable responsible determination of absolute configuration, tissue specific biodisposition and high-throughput screening of multiple amounts of synthetic and naturally occurring stereoisomeric drug-like candidates is still the challenging ability and presents urgent need for pharmaceutical manufacturers [8][9][10][11].…”

Preparation and chromatographic properties of a multimodal chiral stationary phase based on phenyl‐carbamate‐propyl‐β‐CD for HPLC

“…Normal-phase separations typically used hexane or heptane/2-propanol mobile phases. The polar organic mode was primarily developed for the separation of enantiomers that contained hydrogen bonding functional groups on the chiral centre and another functional group in the molecule [12]. It used nonaqueous solvents such as pure ACN or the mixture of ACN/methanol/acetic acid/triethylamine solvents.…”