Gradient elution in hollow-fibre flow field-flow fractionation

Carlshaf, Alf; Jönsson, Jan Åke

doi:10.1016/s0021-9673(00)94277-8

Cited by 28 publications

(20 citation statements)

References 8 publications

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“…It is given by Eq. (19), for any value of the intrinsic viscosity and of the second virial coefficient of the osmotic pressure of the solution or suspension, as well as of the analyte-field interaction parameter at infinite dilution, k o . This expression is useful to predict the direction of variation of retention with sample concentration and to compare it qualitatively with experimental observations when the relevant parameters k and a in Eqs.…”

Section: Resultsmentioning

confidence: 99%

Onset of sample concentration effects on retention in field‐flow fractionation

Martin¹,

Feuillebois²

2003

J of Separation Science

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In field-flow fractionation (FFF), when the sample amount is increased, a shift in retention occurs. Positive as well as negative deviations of the retention have been observed, depending on the type of sample and on the experimental conditions. Two concentration effects have been invoked to explain this retention shift: the viscosity effect (the velocity profile of the analyte suspension or solution is modified by the concentration dependence of the viscosity) and the mean distance effect (the mean distance of the analyte molecules or particles to the accumulation wall and the steady state cross-sectional concentration profile of the analyte are affected by concentration-dependent interactions between analyte molecules or particles). The general FFF retention equation has been solved in the case where the reciprocal of the viscosity and the FFF analyte-field interaction parameter, k, are linearly related to concentration. The two proportionality coefficients involved in these relationships are identified as the intrinsic viscosity and twice the second virial coefficient of the osmotic pressure, respectively. A general expression of the derivative of the analyte average relative velocity, R, with respect to the cross-sectional average analyte concentration, pcP, has been obtained as a function of these two coefficients, and of k o , the limit of k for infinite dilution. A survey of retention effects reported in FFF literature for various sample types and FFF methods is presented.

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Section: Resultsmentioning

confidence: 99%

Onset of sample concentration effects on retention in field‐flow fractionation

Martin¹,

Feuillebois²

2003

J of Separation Science

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“…In addition, HF FlFFF has recently shown that its separation resolution was comparable to that of rectangular type of FlFFF [4]. Earlier studies of HF FlFFF were focused on the use of HF as a channel for FlFFF [1, 2,11]; the potential of the new technique was enlarged with applications to particles, cells, bacteria, and synthetic organic-soluble polymers [12 -17]. Recently, an attempt has been made to interface HF FlFFF with ESI/ MS online for protein separation and simultaneous characterization [7].…”

Section: Introductionmentioning

confidence: 99%

Performance of hollow‐fiber flow field‐flow fractionation in protein separation

Park

Paeng

Kang

et al. 2005

J of Separation Science

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Since hollow-fiber flow field-flow fractionation (HF FIFFF) utilizes a cylindrical channel made of a hollow-fiber membrane, which is inexpensive and simple in channel assembly and thus disposable, interests are increasing as a potential separation device in cells, proteins, and macromolecules. In this study, performance of HF FIFFF of proteins is described by examining the influence of flow rate conditions and length of fiber (polyacrylonitrile or PAN in this work) on sample recovery as well as experimental plate heights. The interfiber reproducibility in terms of separation time and recovery was also studied. Experiments showed that sample recovery was consistent regardless of the length of fiber when the effective field strength (equivalent to the mean flow velocity at the fiber wall) and the channel void time were adjusted to be equivalent for channels of various fiber lengths. This supported that the majority of sample loss in HF FIFFF separation of apoferritin and their aggregates may occur before the migration process. It is finally demonstrated that HF FIFFF can be applied for characterizing the reduction in Stokes' size of low density lipoproteins from blood plasma samples obtained from patients having coronary artery disease and from healthy donors.

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“…In Equation 6, uR is the radial flow velocity component at the fiber wall, R is the radius of the fiber, and D the diffusion coefficient of the sample particles to be separated.…”

Section: Retention In Hollowfibmflow " ' *mentioning

confidence: 99%

“…The particles will travel axially through the channel with a velocity corresponding to their lateral position in the parabolic flow profile that is developed under laminar flow conditions. Flow FFF is the most Universal of the subtechniques in the FFF family, capable of separating molecules and particles at a high level of resolution in a very broad range of molecular weights [2-41. In previous papers [5,6] we presented a novel version of flow FFF in whch the classical parallelplate channel is exchanged for a cylindrical hollow fiber. The set-up used has certain advantages, such as simplicity in operation and independent control of the two flows (elution and cross flow), including an easier way of performing gradient elutions.…”

Section: Introductionmentioning

confidence: 99%

Effects of ionic strength of eluent on retention behavior and on the peak broadening process in hollow fiber flow field‐flow fractionation

Carlshaf

Jönsson

1991

J Microcolumn Separations

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Abstract. The influence of forces on the particles other than the applied cross flow is studied in hollow fiber flow field-flow fractionation (HF'). Such forces can be of either attractive or repelling in nature. It is shown by injecting polystyrene latex beads (0.091 pm in diameter) at different cross flows and by using eluents with different ionic strengths, that there will be disturbances in the concentration profile of the sample irrespective of the type of interacting forces. The retention time and peak broadening were either increased or decreased compared to the theoretical value, depending on the nature of the dominating interaction force. It is, nevertheless, possible to miuimize the total interaction energy between particles and between the particles and the channel wall by choosing a suitable ionic strength.

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Gradient elution in hollow-fibre flow field-flow fractionation

Cited by 28 publications

References 8 publications

Onset of sample concentration effects on retention in field‐flow fractionation

Onset of sample concentration effects on retention in field‐flow fractionation

Performance of hollow‐fiber flow field‐flow fractionation in protein separation

Effects of ionic strength of eluent on retention behavior and on the peak broadening process in hollow fiber flow field‐flow fractionation

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