Gradient distribution pattern of muscarinic receptors in N1E 115 mouse neuroblastoma cells

Abstract

The distribution pattern of muscarinic receptors in N1E 115 mouse neuroblastoma cells after linear and non-linear gradient centrifugation was investigated. In untreated cells, at least two forms of the receptors, with different densities, were found.

Sodium Nitroprusside Induces Internalization of Muscarinic Receptors Stably Expressed in Chinese Hamster Ovary Cell Lines

“…Receptor stimulation by agonists elicits not only a rapid modulation of a second messenger response, but also phosphorylation, internalization, and down-regulation of the receptor itself. The sequestration process can be detected by cell fractionation as a cell surface receptor redistribution into a membrane fraction that exhibits a lower density than that of the plasma membrane (Harden et al ., 1985 ;Fraeyman and Buyse, 1989) . More routinely, this phenomenon is monitored as a reduction in the number of binding sites for hydrophilic ligands in intact cells after agonist stimulation (Feigenbaum and El-Fakahany, 1985 ;Baumgold et al ., 1989 ;Cioffi and El-Fakahany, 1989 ; Thompson and Fisher, 1990) .…”

Sequestration of Muscarinic Cholinergic Receptors in Permeabilized Neuroblastoma Cells

“…Agonist occupancy of neural muscarinic acetylcholine receptors (mAChRs) elicits not only a rapid modulation of either adenylyl cyclase or phosphoinositidase C (PIC) activities, but also a slower adaptive response in which the cell surface receptors are internalized into a more hydrophobic cell compart-ment. The latter process, termed receptor sequestration, can be monitored following cell fractionation as a redistribution of cell surface receptors into a membrane fraction that exhibits a lower density than that of the plasma membrane (Harden et al, 1985;Fraeyman and Buyse, 1989). However, it is more routinely detected as an agonist-induced reduction in the number of binding sites for hydrophilic ligands in intact cells (Fiegenbaum and El-Fakahany, 1985;Baumgold et al, 1989;Cioffi and El-Fakahany, 1989; Thompson and Fisher, 1990).…”