Graded replacement of maize grain with molassed sugar beet pulp modulated ruminal microbial community and fermentation profile <i>in vitro</i>

Münnich, Matthias; Khol-Parisini, A.; Klevenhusen, Fenja; Metzler‐Zebeli, Barbara U.; Zebeli, Qendrim

doi:10.1002/jsfa.8547

Cited by 10 publications

(9 citation statements)

References 38 publications

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“…Comparatively less research attention, however, has been dedicated to rumen microbial changes on beet pulp-based diets and to our knowledge, no studies have utilized BP as a replacement for forage in the diet and compared differences in the microbial profiles. Studies have instead focused on using BP as a highfiber/low-starch energy source to replace starchy concentrate feeds (Zhao et al, 2013;Münnich et al, 2018). Nonetheless, these studies have still observed reductions in fiber-degrading bacterial populations in response to BP treatment, which is consistent with our findings (Zhao et al, 2013;Münnich et al, 2018).…”

Section: Dietsupporting

confidence: 87%

“…Studies have instead focused on using BP as a highfiber/low-starch energy source to replace starchy concentrate feeds (Zhao et al, 2013;Münnich et al, 2018). Nonetheless, these studies have still observed reductions in fiber-degrading bacterial populations in response to BP treatment, which is consistent with our findings (Zhao et al, 2013;Münnich et al, 2018). These results suggest that the optimal type of fiber substrate likely differs between various bacterial populations involved in its breakdown and that fibrolytic species may not be supported as adequately by non-forage fiber sources.…”

Section: Dietsupporting

confidence: 84%

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Characterizing Effects of Ingredients Differing in Ruminally Degradable Protein and Fiber Supplies on the Ovine Rumen Microbiome Using Next-Generation Sequencing

et al. 2021

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The ratio of concentrate to forage within diets is known to alter rumen microbial profiles, but comparatively less information is available on the effect of differing sources of individual nutrients on the microbiome. The objective of this study was to investigate rumen microbial responses to diets composed of protein and fiber sources expected to vary in nutrient degradability. The responses of interest included relative abundances of bacterial taxa as well as estimations of community richness and diversity. Ten ruminally cannulated wethers (Suffolk, Dorset, or Suffolk × Dorset) received four diet treatments consisting of either beet pulp or timothy hay and soybean meal (SBM) or heat-treated soybean meal (HSBM) in a partially replicated 4 × 4 Latin square experiment for 21 days. Timothy hay and beet pulp were expected to provide differing rumen degradabilities of neutral detergent fiber (NDF) while the soybean meals were expected to provide differing rumen degradabilities of crude protein (CP). Solid and liquid samples of rumen contents were collected for microbial DNA isolation and Next-Generation sequencing. Numerous rumen bacterial population shifts were observed due to change in fiber source, with increased abundances (P < 0.05) of fibrolytic populations associated with timothy hay diets compared with beet pulp diets. Conversely, populations of the pectin-degrading genera, Treponema and Lachnospira, increased on the beet pulp treatment (P = 0.015 and P = 0.0049, respectively). Limited impact on bacterial taxa was observed between diets differing in protein source. The Paraprevotellaceae genus YRC22 was observed to increase in abundance on HSBM diets (P = 0.023) and the phylum Spirochaetes tended to be more abundant on SBM than HSBM diets (P = 0.071). Beet pulp decreased rumen bacterial diversity (P = 0.0027) and tended to decrease bacterial species richness (P = 0.051) compared to timothy hay. Our results serve to further underscore the sensitivity of rumen microbes to changes in their preferred substrates, particularly of those associated with fiber degradation.

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Section: Dietsupporting

confidence: 87%

Section: Dietsupporting

confidence: 84%

Characterizing Effects of Ingredients Differing in Ruminally Degradable Protein and Fiber Supplies on the Ovine Rumen Microbiome Using Next-Generation Sequencing

et al. 2021

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“…A low ruminal pH is strongly associated with high-grain feeding (Zebeli et al, 2008a), possibly resulting in SARA, which is defined as intermittent drops below pH 5.8 for more than 5.24 h per day (Zebeli et al, 2008b) or below pH 6.0 if measured in the reticulum with indwelling sensors (Neubauer et al, 2018). A previous in vitro study found that the replacement of corn grain with Bp does not reduce the fermentative activity in the rumen and the amount of SCFA produced (Münnich et al, 2018b). Thus a similar acidic potential can be assumed, which confirms the results found by Voelker and Allen (2003b), who found no difference in ruminal pH when diets with varying proportions of Bp and corn were fed.…”

Section: Effects On Rumination and Reticuloruminal Phmentioning

confidence: 99%

Graded replacement of corn grain with molassed sugar beet pulp modulates the fecal microbial community and hindgut fermentation profile in lactating dairy cows

Petri

Münnich

Zebeli

et al. 2019

Journal of Dairy Science

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High starch lactation diets not only enhance the risk of subacute ruminal acidosis but also of hindgut acidosis, which increases the risk of dysbiosis and the depression of fiber degradation. We recently showed that replacing corn with molassed sugar beet pulp (Bp) improved fiber degradation in high-producing dairy cattle, possibly because of an improvement of rumen and hindgut conditions for microbes by Bp feeding. However, little is known about the effects of high inclusion rates of Bp on hindgut microbes and fermentation. Thus fecal grab samples were taken from 18 high-yielding Simmental cows after 28 d of feeding 3 different levels of Bp (n = 6) for bacterial 16S rRNA amplicon sequencing. In addition, the reticular pH was continuously monitored with indwelling sensors and eating and ruminating behavior was evaluated with noseband sensors. The Bp inclusion rates were 0 g/kg (i.e., no Bp inclusion as control, CON), 120 g/kg (12Bp), or 240 g/kg (24Bp) replacing corn grain and limestone on a dry matter basis. The amount of time spent eating and ruminating was unaffected by Bp level, and the daily fluctuation in the reticular pH was reduced by 25% with Bp inclusion from 0.8 in the CON diet to 0.6 in 24Bp fed animals. Also, the fecal pH tended to increase with dietary Bp inclusion. Fecal acetate production showed a quadratic tendency with the lowest concentration (58.9%) of the total short-chain fatty acid in the 12Bp treatment. Inclusion of Bp up to 24% of the diet decreased the fecal butyrate proportion by 27%. The Shannon diver-sity index was increased from 5.50 to 8.09 with dietary Bp inclusion indicating increased species diversity. Of the 200 most abundant operational taxonomic units, 25 were increased by dietary Bp inclusion, whereas 15 were decreased and 7 were quadratically affected. The second most abundant group was proposed taxon "CF231" of the family Paraprevotellaceae. Although it accounted for only 2.52% of the operational taxonomic units in the CON diet, it was increased by 64% with dietary Bp inclusion. The largest relative change in the abundance was found for the genus Fibrobacter that increased more than 14-fold from 0.04% (CON) to 0.66% (24Bp). In conclusion, feeding molassed sugar beet pulp as partial substitution of corn up to 240 g/ kg is a viable alternative that promotes ruminal and hindgut fermentation by supporting physiological pH and bacterial diversity.

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“…However, Pragya (2016) reported that by replacing maize fodder with different levels of sugar beet pulp (0:100, 20:80, 40:60, 60:40, 80:20 and 100:0), there was increase (P<0.05) in butyric acid proportion with increased level of sugar beet pulp. In other study, Munnich et al (2017) reported that there was increased proportion of butyric acid when maize grains were replaced with sugar beet pulp.…”

Section: Individual Volatile Fatty Acidsmentioning

confidence: 92%

“…However, acetic acid in concentrate mixture 4 (60% DSBP) and concentrate mixture 5 (80% DSBP) was similar. However, Munnich et al (2017) reported that acetic acid concentration was not affected when maize grains were replaced with dried sugar beet pulp. In other study, Pragya (2016) reported that the production of acetic acid increased (P<0.05) with increased supplementation of ensiled sugar beet pulp replacing maize fodder in vitro.…”

Section: Individual Volatile Fatty Acidsmentioning

confidence: 99%

In vitro Nutrient Digestibility and Fermentation Pattern of Concentrate Mixtures Containing Incremental Levels of Sugar Beet Pulpin Goat Inoculum

Kaur¹,

Grewal²,

Chawla³

2018

Int.J.Curr.Microbiol.App.Sci

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Graded replacement of maize grain with molassed sugar beet pulp modulated ruminal microbial community and fermentation profile in vitro

Cited by 10 publications

References 38 publications

Characterizing Effects of Ingredients Differing in Ruminally Degradable Protein and Fiber Supplies on the Ovine Rumen Microbiome Using Next-Generation Sequencing

Characterizing Effects of Ingredients Differing in Ruminally Degradable Protein and Fiber Supplies on the Ovine Rumen Microbiome Using Next-Generation Sequencing

Graded replacement of corn grain with molassed sugar beet pulp modulates the fecal microbial community and hindgut fermentation profile in lactating dairy cows

In vitro Nutrient Digestibility and Fermentation Pattern of Concentrate Mixtures Containing Incremental Levels of Sugar Beet Pulpin Goat Inoculum

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