GPR39 activates proliferation and differentiation of porcine intramuscular preadipocytes through targeting the PI3K/AKT cell signaling pathway

Dong, Xiaoying; Tang, Sheng-Qiu; Zhang, Wei; Gao, Weihua; Chen, Yanfei

doi:10.3109/10799893.2015.1056308

Cited by 34 publications

(25 citation statements)

References 42 publications

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“…The K2 cluster included genes that showed a notable overall trend of upregulation, suggesting their key roles over the entire process of differentiation. Members of this cluster, such as GPR39 and CHCHD4 , reportedly regulate the differentiation of porcine intramuscular preadipocytes (Dong et al 2016). However, their functions in chicken preadipocytes require experimental verification.…”

Section: Discussionmentioning

confidence: 99%

Genome-Wide Analysis of lncRNA and mRNA Expression During Differentiation of Abdominal Preadipocytes in the Chicken

Zhang

Han

et al. 2017

G3 Genes|Genomes|Genetics

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Long noncoding RNAs (lncRNAs) regulate adipogenesis and other processes associated with metabolic tissue development and function. However, little is known about the function and profile of lncRNAs during preadipocyte differentiation in the chicken (Gallus gallus). Herein, lncRNA and mRNA expression in preadipocytes at different stages of differentiation were analyzed using RNA sequencing. A total of 1,300,074,528 clean reads and 27,023 novel lncRNAs were obtained from 12 samples. The number of genes (1336 lncRNAs and 1759 mRNAs; 3095 in total) differentially expressed across various stages declined as differentiation progressed. Differentially expressed genes were found to be involved in several pathways related to preadipocyte differentiation that have been extensively studied, including glycerolipid metabolism, and the mammalian target of rapamycin, peroxisome proliferator-activated receptor, and mitogen-activated protein kinase signaling pathways. To our knowledge, some pathways are being reported for the first time, including the propanoate metabolism, fatty acid metabolism, and oxidative phosphorylation pathways. Furthermore, 3095 differentially expressed genes were clustered into eight clusters, and their expression patterns were determined through K-means clustering. Genes involved in the K2 cluster likely play important roles in preadipocyte differentiation. Six stage-specific modules related to A0 (day 0), A2 (day 2), and A6 (day 6) stages were identified, using weighted coexpression network analysis. Nine central, highly connected .genes in stage-specific modules were subsequently identified, including XLOC_068731, XLOC_022661, XLOC_045161, XLOC_070302, CHD6, LLGL1, NEURL1B, KLHL38, and ACTR6. This study provides a valuable resource for further study of chicken lncRNA and facilitates a better understanding of preadipocyte differentiation in the chicken

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Section: Discussionmentioning

confidence: 99%

Genome-Wide Analysis of lncRNA and mRNA Expression During Differentiation of Abdominal Preadipocytes in the Chicken

Zhang

Han

et al. 2017

G3 Genes|Genomes|Genetics

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“…Particular attention was paid to K2 cluster which included genes that underwent an overall trend of increase, suggesting their key roles over the entire differentiation process. Members from this cluster such as GPR39 and CHCHD4 had been reported to regulate the differentiation of preadipocytes(Dong et al, 2016).…”

Section: Discussionmentioning

confidence: 99%

“…Interestingly, we found that the functions of genes migrated from RNA editing at A0 stage, to generation of precursor metabolites and energy at A2 stage and finally to regulation of cell death and apoptosis at A6 stage. As far as we know, quite a few pathways involved in preadipocytes differentiation have been validated to date, including the PI3K/AKT cell signaling pathway(Dong et al, 2016), LKB1-AMPK Pathway(Tung et al, 2016), Wnt/β-catenin signaling pathway(Lu et al, 2016; Mai et al, 2014; Zhang et al, 2014c), TGF-β pathway(Park et al, 2014), Bmp/Smad pathway(Liu et al, 2014; Suenaga et al, 2013), ERK signaling pathway(Chiang et al, 2013), PDK1/Akt pathway(He et al, 2013), p38 MAPK/ATF-2 and TOR/p70 S6 kinase pathways(Yan et al, 2013). However, little is known about pathways involved in preadipocytes differentiation of chicken.…”

Section: Discussionmentioning

confidence: 99%

Genome-wide analysis of lncRNAs and mRNAs expression during the differentiation of abdominal preadipocytes in chicken

Zhang

Han

et al. 2016

Preprint

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AbstractlncRNAs regulate metabolic tissue development and function, including adipogenesis. However, little is known about the function and profile of lncRNAs in preadipocytes differentiation of chicken. Here, we identified lncRNAs in preadipocytes of different differentiation stages by RNA-sequencing using Jinghai Yellow chicken. A total of 1,300,074,528 clean reads and 27,023 lncRNAs were obtained from twenty samples. 3095 genes (1,336 lncRNAs and 1,759 mRNAs) were differentially expressed among different stages, of which the number of DEGs decreased with the differentiation, demonstrating that the early stage might be most important for chicken preadipocytes differentiation. Furthermore, 3,095 DEGs were clustered into 8 clusters with their expression patterns by K-means clustering. We identified six stage-specific modules related to A0, A2 and A6 stages using weighted co-expression network analysis. Many well-known/novel pathways associated with preadipocytes differentiation were found. We also identified highly connected genes in each module and visualized them by cytoscape. Many well-known genes related to preadipocytes differentiation were found such as IGFBP2 and JUN. Yet, the majority of high connected genes were unknown in chicken preadipocytes. This study provides a valuable resource for chicken lncRNA study and contributes to batter understanding the biology of preadipocytes differentiation in chicken.

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“…VEPCs at a density of 1.0×10 4 /ml were collected from the cultures and replated into a 96-well plate and underwent different treatments. Cell proliferation was determined using MTT method according to the previous study (Dong et al 2016). A microplate reader was used to measure the absorbance at 450 nm (Bio-Rad, Hercules, CA, USA).…”

Section: Mtt Assaymentioning

confidence: 99%

“…Expression of Bcl-2 and VEGF in VEPCs was determined at indicated times by RNA preparation and quantitative reverse transcription polymerase chain reaction (RT-PCR). Total cellular RNA was isolated from cells on 6-well plates using Trizol reagent following the manufacturer's instructions (Invitrogen, Carlsbad, CA, USA) and RT-PCR was performed according to the reference (Dong et al 2016). β-actin expression was used as an internal control.…”

Section: Gene Expression Analysismentioning

confidence: 99%

Adiponectin Enhances Biological Functions of Vascular Endothelial Progenitor Cells Through the mTOR-STAT3 Signaling Pathway

Xiao-ying

Xia²,

Zhang³

et al. 2018

Physiol Res

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Adiponectin (APN), an adipose tissue-excreted adipokine, plays protective roles in metabolic and cardiovascular diseases. In this study, the effects and mechanisms of APN on biological functions of rat vascular endothelial progenitor cells (VEPCs) were investigated in vitro. After administrating APN in rat VEPCs, the proliferation was measured by methyl thiazolyl tetrazolium (MTT) method, the apoptotic rate was test by Flow cytometry assay, mRNA expression of B-cell lymphoma-2 (Bcl-2) and vascular endothelial growth factor (VEGF) was determined by real-time reverse transcriptase polymerase chain reaction (RT-PCR), and protein expression of mechanistic target of rapamycin (mTOR), signal transducer and activator of transcription 3 (STAT3) and phospho-STAT3 (pSTAT3) was analyzed by Western blot. It was suggested that APN promoted the optical density (OD) value of VEPCs, enhanced mRNA expression of Bcl-2 and VEGF, and inhibited cell apoptotic rate. Furthermore, protein expression of pSTAT3 was also increased in the presence of APN. Moreover, APN changed-proliferation, apoptosis and VEGF expression of VEPCs were partially suppressed after blocking the mTOR-STAT3 signaling pathway by the mTOR inhibitor XL388. It was indicated that APN promoted biological functions of VEPCs through targeting the mTOR-STAT3 signaling pathway.

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GPR39 activates proliferation and differentiation of porcine intramuscular preadipocytes through targeting the PI3K/AKT cell signaling pathway

Abstract: It indicated that GPR39 was a transducer of Zn(2+), and enhanced proliferation and differentiation of porcine intramuscular preadipocytes through activation of the PI3K/Akt signaling pathway.

Cited by 34 publications

References 42 publications

Genome-Wide Analysis of lncRNA and mRNA Expression During Differentiation of Abdominal Preadipocytes in the Chicken

Genome-Wide Analysis of lncRNA and mRNA Expression During Differentiation of Abdominal Preadipocytes in the Chicken

Genome-wide analysis of lncRNAs and mRNAs expression during the differentiation of abdominal preadipocytes in chicken

Adiponectin Enhances Biological Functions of Vascular Endothelial Progenitor Cells Through the mTOR-STAT3 Signaling Pathway

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