GPI anchors: Regulated as needed

Aguilera-Romero, Auxiliadora; Muñiz, Manuel

doi:10.1083/jcb.202303097

Cited by 2 publications

(2 citation statements)

References 9 publications

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“…Studies indicated a crucial role of GPI-TA in regulating glycolipid biosynthesis, since GPI biosynthesis is suppressed by the ER-associated degradation pathway when cells are defective in transferring the complete GPI core to proteins to prevent GPI accumulation ( 9 , 45 ). Biosynthesis of GPI is upregulated in ER-associated degradation-deficient cells ( 46 ).…”

Section: Key Roles Of Pigm Pigx ...mentioning

confidence: 99%

Role of PIGM and PIGX in glycosylphosphatidylinositol biosynthesis and human health (Review)

Torres‑Valdetano,

Vallejo‑Ruiz,

Milflores‑Flores

et al. 2024

Biomed Rep

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Glycosylphosphatidylinositol-glycan (GPI) is an anchor to specific cell surface proteins known as GPI-anchored proteins (APs) that are localized in lipid rafts and may act as cell co-receptors, enzymes and adhesion molecules. The present review investigated the significance of GPI biosynthesis class phosphatidylinositol-glycan (PIG)M and PIGX in GPI synthesis and their implications in human health conditions. PIGM encodes GPI-mannosyltransferase I (MT-I) enzyme that adds the first mannose to the GPI core structure. PIGX encodes the regulatory subunit of GPI-MT-I. The present review summarizes characteristics of the coding sequences of PIGM and PIGX , and their expression in humans, as well as the relevance of GPI-MT-I and the regulatory subunit in maintaining the presence of GPI-APs on the cell surface and their secretion. In addition, the association of PIGM mutations with paroxysmal nocturnal hemoglobinuria and certain types of GPI-deficiency disease and the altered expression of PIGM and PIGX in cancer were also reviewed. In addition, their interaction with other proteins was described, suggesting a complex role in cell biology. PIGM and PIGX are critical genes for GPI synthesis. Understanding gene and protein regulation may provide valuable insights into the role of GPI-APs in cellular processes.

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Section: Key Roles Of Pigm Pigx ...mentioning

confidence: 99%

Role of PIGM and PIGX in glycosylphosphatidylinositol biosynthesis and human health (Review)

Torres‑Valdetano,

Vallejo‑Ruiz,

Milflores‑Flores

et al. 2024

Biomed Rep

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“…Because of its role in the conserved post-transcription modification processes, cell surface glycosylphosphatidylinositol-anchoring technology is expected to aid the construction of novel functional yeast cells with oriented protein transportation and accurate colocalization. Their construction depends on the functional proteins that reside on the plasma membrane ( Inokuma et al, 2021 ; Aguilera-Romero and Muñiz, 2023 ).…”

Section: Conclusion and Perspectivementioning

confidence: 99%

An outlook to sophisticated technologies and novel developments for metabolic regulation in the Saccharomyces cerevisiae expression system

Wu,

Feng,

Sun

et al. 2023

Front. Bioeng. Biotechnol.

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Saccharomyces cerevisiae is one of the most extensively used biosynthetic systems for the production of diverse bioproducts, especially biotherapeutics and recombinant proteins. Because the expression and insertion of foreign genes are always impaired by the endogenous factors of Saccharomyces cerevisiae and nonproductive procedures, various technologies have been developed to enhance the strength and efficiency of transcription and facilitate gene editing procedures. Thus, the limitations that block heterologous protein secretion have been overcome. Highly efficient promoters responsible for the initiation of transcription and the accurate regulation of expression have been developed that can be precisely regulated with synthetic promoters and double promoter expression systems. Appropriate codon optimization and harmonization for adaption to the genomic codon abundance of S. cerevisiae are expected to further improve the transcription and translation efficiency. Efficient and accurate translocation can be achieved by fusing a specifically designed signal peptide to an upstream foreign gene to facilitate the secretion of newly synthesized proteins. In addition to the widely applied promoter engineering technology and the clear mechanism of the endoplasmic reticulum secretory pathway, the innovative genome editing technique CRISPR/Cas (clustered regularly interspaced short palindromic repeats/CRISPR-associated system) and its derivative tools allow for more precise and efficient gene disruption, site-directed mutation, and foreign gene insertion. This review focuses on sophisticated engineering techniques and emerging genetic technologies developed for the accurate metabolic regulation of the S. cerevisiae expression system.

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GPI anchors: Regulated as needed

Cited by 2 publications

References 9 publications

Role of PIGM and PIGX in glycosylphosphatidylinositol biosynthesis and human health (Review)

Role of PIGM and PIGX in glycosylphosphatidylinositol biosynthesis and human health (Review)

An outlook to sophisticated technologies and novel developments for metabolic regulation in the Saccharomyces cerevisiae expression system

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