GPH1 is involved in glycerol accumulation in the three-dimensional networks of the nematode-trapping fungus Arthrobotrys oligospora

Wu, Qin-Yi; Zhu, Yueyan; Zou, Cheng‐Gang; Kang, Yingqian; Liang, Lianming

doi:10.1007/s12275-016-6272-8

Cited by 7 publications

(2 citation statements)

References 23 publications

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“…For example, a recent work has generated a strain lacking the signaling scaffold protein Soft and shown that cell-cell fusion is required for ring closure in Duddingtonia flagrans (41). In A. oligospora , ATCC24927-background trapping-deficient mutants have been isolated: adhesin protein Mad1 (42), autophagy protein Atg8 (43), mitogen-activated protein kinase Slt2 (44), pH sensor PalH (45), NADPH oxidase NoxA (46), low-affinity calcium uptake system proteins Fig1 and Fig2 (47), Rab GTPase Rab7A (48), actin-associated protein Crn1 (49), Woronin body component Hex1 (50), malate synthase Mls (51), glycogen phosphorylase Gph1 (52), transcription factors VelB (53) and StuA (54), and microRNA processing protein Qde2 (55), to which we add the G-protein β subunit Gpb1. However, a mechanistic view of how these and other molecular players are interconnected during the interaction of A. oligospora with their prey is totally lacking.…”

Section: Resultsmentioning

confidence: 99%

Natural diversity in the predatory behavior facilitates the establishment of a new robust model strain for nematode-trapping fungi

Yang

Ulzurrun

Gonçalves

et al. 2019

Preprint

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33Nematode-trapping fungi (NTF) are a group of specialized microbial predators that consume 34 nematodes when food sources are limited. Predation is initiated when conserved nematode 35 ascaroside pheromones are sensed, followed by the development of complex trapping devices. 36To gain insights into the co-evolution of this inter-kingdom predator-prey relationship, we 37 investigated natural populations of nematodes and NTF, that we found to be ubiquitous in soils. 38Arthrobotrys species were sympatric with various nematode species and behaved as generalist 39 predators. The ability to sense prey amongst wild isolates of A. oligospora varied greatly, as 40 determined by the number of traps after exposure to Caenorhabditis elegans. While some strains 41 were highly sensitive to C. elegans and the nematode pheromone ascarosides, others responded 42 only weakly. Furthermore, strains that were highly sensitive to the nematode prey also developed 43 traps faster. The polymorphic nature of trap formation correlated with competency in prey 44 killing, as well as with the phylogeny of A. oligospora natural strains, calculated after assembly 45 and annotation of the genomes of twenty isolates. A chromosome level genome assembly and 46 annotation was established for one of the most sensitive wild isolate, and deletion of the only G 47 protein b subunit-encoding gene of A. oligospora nearly abolished trap formation, implicating G 48 protein signaling in predation. In summary, our study establishes a highly responsive A. 49 oligospora wild isolate as a novel model strain for the study of fungal-nematode interactions and 50 demonstrates that trap formation is a fitness character in generalist predators of the NTF family. 51 52 Significance statement 53Nematode-trapping fungi (NTF) are carnivorous microbes that hold potential to be used 54 as biological control agents due to their ability to consume nematodes. In this work we show that 55 NTF are ubiquitous generalist predators found in sympatry with their prey in soil samples. Wild 56 isolates of NTF displayed a naturally diverse ability to execute their predatory lifestyle. We 57 generated a large whole genome sequencing dataset for many of the fungal isolates that will 58 serve as the basis of future projects isolates. In particular, we establish TWF154, a highly 59 responsive strain of Arthrobotrys oligospora, as a model strain to study the genetics of NTF. 60Lastly, we provide evidence that G-protein signaling is necessary for trap induction in NTF. 61 62 63 64The living component of soils are in permanent interaction and play central roles in 65 various aspects of biogeochemistry, including nutrient cycling and transport across distances (1). 66Specifically, nematodes are the most abundant animals in nature, amounting to a staggering 0.3 67 gigatonnes or 4.5 x 10 20 individuals, many of which are devastating parasites of plants, animals 68 and humans (2). Although available, nematicide chemicals pose severe environmental and health 69 risks and therefore, biological cont...

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Section: Resultsmentioning

confidence: 99%

Natural diversity in the predatory behavior facilitates the establishment of a new robust model strain for nematode-trapping fungi

Yang

Ulzurrun

Gonçalves

et al. 2019

Preprint

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“…In addition, mutations in latg13 , atg1 , atg4 , and atg5 led to the loss of ability for trap formation in A. oligospora [ 77 , 78 , 79 , 80 ]. Besides autophagy, some cellular processes affect the formation of traps in A. oligospora , such as woronin body synthesis [ 81 ], RNA interference [ 82 ], glycerol biosynthesis [ 83 ], production of reactive oxygen species [ 84 ], F-box protein synthesis [ 85 ], nitrate assimilation pathway [ 86 ], pH-sensing receptor protein synthesis [ 87 ], velvet family protein synthesis [ 88 ], scaffold protein synthesis [ 89 ], lectin synthesis [ 90 ], actin synthesis [ 91 ], and malate synthase [ 92 ]. Finally, research confirms that A. oligospora possesses pathways related to the biosynthesis of SECs of the gene cluster AOL_s00215g, containing 11 genes.…”

Section: Mechanisms Of Trap Formation In a Oligosporamentioning

confidence: 99%

Recent Advances in Life History Transition with Nematode-Trapping Fungus Arthrobotrys oligospora and Its Application in Sustainable Agriculture

Wang

Rao

et al. 2023

Pathogens

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Parasitic nematodes cause great annual loss in the agricultural industry globally. Arthrobotrys oligospora is the most prevalent and common nematode-trapping fungus (NTF) in the environment and the candidate for the control of plant- and animal-parasitic nematodes. A. oligospora is also the first recognized and intensively studied NTF species. This review highlights the recent research advances of A. oligospora as a model to study the biological signals of the switch from saprophytism to predation and their sophisticated mechanisms for interacting with their invertebrate hosts, which is of vital importance for improving the engineering of this species as an effective biocontrol fungus. The application of A. oligospora in industry and agriculture, especially as biological control agents for sustainable purposes, was summarized, and we discussed the increasing role of A. oligospora in studying its sexual morph and genetic transformation in complementing biological control research.

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Natural diversity in the predatory behavior facilitates the establishment of a robust model strain for nematode-trapping fungi

Yang

Ulzurrun

Gonçalves

et al. 2020

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

Nematode-trapping fungi (NTF) are a group of specialized microbial predators that consume nematodes when food sources are limited. Predation is initiated when conserved nematode ascaroside pheromones are sensed, followed by the development of complex trapping devices. To gain insights into the coevolution of this interkingdom predator–prey relationship, we investigated natural populations of nematodes and NTF that we found to be ubiquitous in soils.Arthrobotrysspecies were sympatric with various nematode species and behaved as generalist predators. The ability to sense prey among wild isolates ofArthrobotrys oligosporavaried greatly, as determined by the number of traps after exposure toCaenorhabditis elegans. While some strains were highly sensitive toC. elegansand the nematode pheromone ascarosides, others responded only weakly. Furthermore, strains that were highly sensitive to the nematode prey also developed traps faster. The polymorphic nature of trap formation correlated with competency in prey killing, as well as with the phylogeny ofA. oligosporanatural strains, calculated after assembly and annotation of the genomes of 20 isolates. A chromosome-level genome assembly and annotation were established for one of the most sensitive wild isolates, and deletion of the only G-protein β-subunit–encoding gene ofA. oligosporanearly abolished trap formation. In summary, our study establishes a highly responsiveA. oligosporawild isolate as a model strain for the study of fungus–nematode interactions and demonstrates that trap formation is a fitness character in generalist predators of the nematode-trapping fungus family.

show abstract

GPH1 is involved in glycerol accumulation in the three-dimensional networks of the nematode-trapping fungus Arthrobotrys oligospora

Cited by 7 publications

References 23 publications

Natural diversity in the predatory behavior facilitates the establishment of a new robust model strain for nematode-trapping fungi

Natural diversity in the predatory behavior facilitates the establishment of a new robust model strain for nematode-trapping fungi

Recent Advances in Life History Transition with Nematode-Trapping Fungus Arthrobotrys oligospora and Its Application in Sustainable Agriculture

Natural diversity in the predatory behavior facilitates the establishment of a robust model strain for nematode-trapping fungi

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