GPGMH, a New Fixed Timed-AI Synchronization Regimen for Swamp and River Crossbred Buffaloes (Bubalus bubalis)

Abulaiti, Adili; El-Qaliouby, Hadeel S.; Bahgy, Halla E.K. El; Naseer, Zahid; Ahmed, Zulfiqar; Hua, Guohua; Yang, Liguo

doi:10.3389/fvets.2021.646247

Cited by 5 publications

(9 citation statements)

References 33 publications

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“…The hypothesis of the present experiment was based on a preliminary study conducted [ 24 ] using crossbred buffaloes which reported improved reproductive parameters when hCG and mifepristone were included in the conventional OVS protocol. In the present study, nulli- and multiparous buffaloes were synchronized by the GPGMH and OVS protocol during different seasons.…”

Section: Discussionmentioning

confidence: 99%

“…It has been reported that CL formed after hCG use has a similar steroidogenic activity on the progesterone hormone [ 26 ]. Additionally, its usage increases the functional CL area for the optimum progesterone level [ 24 ]. Also, the low response of heifers to GPGMH or OVS compared to multiparous buffaloes could be the result of the use of an inappropriate body weight of buffalo heifers or stress occurring during rigorous rectal palpation for ovarian dynamics, which led to low follicular output.…”

Section: Discussionmentioning

confidence: 99%

“…In this experiment, GPGMH protocol was shown as Figure 1 [ 24 ]). Animals were muscularly injected with the first GnRH (gonadotropin-releasing hormone, 400 μg on day 0, PGF 2 α (prostaglandin F 2 α, 0.5 mg) on day 7, the 2nd GnRH and mifepristone (0.4 mg/kg) simultaneously on day 9, and hCG (human chorionic gonadotropin, 2000IU,) on day 5 after AI.…”

Section: Methodsmentioning

confidence: 99%

“… The schematic description of the modified Ovsynch protocol (GPGMH) applied in nulliparous and multiparous buffaloes during peak and low breeding seasons [ 24 ]. …”

Section: Figurementioning

confidence: 99%

“…Decreased progesterone concentrations at the time of AI and greater CL volume are prerequisites for future pregnancy establishment in buffaloes [ 23 ]. Our previous study showed that the inclusion of mifepristone and hCG in the modified OVS protocol (GPGMH) could improve the fertility by enhancing the ovulation rates and subsequent conception rate in tested buffaloes [ 24 ]. Mifepristone is a kind of progesterone (P4) receptor antagonist that works as an antiprogestogen by blocking the progesterone receptor, in turn rapidly reducing the P4 level, and further promoting the luteinizing hormone (LH) surge for ovulation [ 25 ].…”

Section: Introductionmentioning

confidence: 99%

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Effect of Different Synchronization Regimens on Reproductive Variables of Crossbred (Swamp × Riverine) Nulliparous and Multiparous Buffaloes during Peak and Low Breeding Seasons

Abulaiti

Naseer

Ahmed

et al. 2022

Animals

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The present study was conducted to examine the effect of conventional the Ovsynch protocol (OVS) and a modified Ovsynch synchronization (GPGMH) protocol on the follicular dynamics, estrus, ovulation, and pregnancy in nulliparous and multiparous crossbred (swamp × riverine) buffaloes during different seasons. GPGMH or OVS protocols were used to synchronize nulliparous (n = 128; GPGMH = 94, OVS = 34) and multiparous (n = 154; GPGMH = 122, OVS = 32) buffaloes during the peak (n = 186; GPGMH = 143, OVS = 43) and low breeding (n = 96; GPGMH = 73, OVS = 23) seasons. Buffaloes were monitored for follicular dynamics, estrus response, ovulation, and pregnancy rates. The results showed that protocol, parity, and season had significant effects on estrus, ovulation, and pregnancy variables, and interactions among parity and protocol, season and protocol, and season and parity were observed for few of reproductive indices in the crossbred buffaloes. There were no significant (p > 0.05) interaction for protocol, parity and season. In multiparous buffaloes, the application of the GPGMH protocol significantly (p < 0.05) increased the interaction to the interval to estrus onset after the second GnRH, estrus response, ovulation rate, and pregnancy rate, and lowered (p < 0.05) the silent estrus when compared with the conventional OVS protocol. During the peak breeding season, the application of the GPGMH protocol significantly (p < 0.05) improved the interaction to the estrus response, ovulation rate, and pregnancy rate, while it lowered (p < 0.05) the silent estrus incidence when compared to the conventional OVS protocol. In conclusion, the GPGMH protocol, in comparison to the OVS protocol, improves the follicular dynamics, estrus response, ovulation, and pregnancy rates in crossbred multiparous buffaloes during the peak breeding seasons.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

“… The schematic description of the modified Ovsynch protocol (GPGMH) applied in nulliparous and multiparous buffaloes during peak and low breeding seasons [ 24 ]. …”

Section: Figurementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Effect of Different Synchronization Regimens on Reproductive Variables of Crossbred (Swamp × Riverine) Nulliparous and Multiparous Buffaloes during Peak and Low Breeding Seasons

Abulaiti

Naseer

Ahmed

et al. 2022

Animals

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Nasal immunization with AMH-INH-RFRP DNA vaccine for improving follicle development and fertility in buffaloes

Chen

Zhao²,

An³

et al. 2023

Front. Endocrinol.

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IntroductionInhibin DNA vaccine has already been proven to improve the fertility of animals. This study aimed to investigate the effects of a novel Anti-Müllerian hormone (AMH)-Inhibin (INH)-RF-amide-related peptides (RFRP) DNA vaccine on immune response and reproductive performance in buffalo.MethodsA total of 84 buffaloes were randomly divided into four groups and nasally immunized twice a day with 10 ml of either AMH-INH-RFRP DNA vaccines (3 × 1010 CFU/ml in group T1, 3 × 109 CFU/ml in group T2, and 3 × 108 CFU/ml in group T3) or PBS (as a control) for 3 days, respectively. All animals received a booster dose at an interval of 14 days.ResultsELISA assay revealed that primary and booster immunization significantly increased the anti-AMH, anti-INH, and anti-RFRP antibody titers in the T2 group compared with that in the T3 group. After the primary immunization, the antibody positive rate was significantly higher in the T2 group than that in the T3 group. In addition, ELISA results indicated that concentrations of E2, IFN-γ, and IL-4 were significantly higher in the antibody-positive (P) group compared to the antibody-negative (N) group. In contrast, there was no significant difference in the concentrations of P4 between the P and N groups. Ultrasonography results revealed a highly significant increase of 2.02 mm in the diameter of ovulatory follicles in the P group compared to the N group. In parallel, growth speed of dominant follicles was significantly higher in the P group than that in the N group (1.33 ± 1.30 vs 1.13 ± 0.12). Furthermore, compared to N group, the rates of oestrus, ovulation, and conception were also significantly higher in the P group.ConclusionThe novel AMH-INH-RFRP DNA vaccine improves the proportion of oestrus, ovulation, and conception in buffalo by promoting the production of E2 and the growth of follicles.

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Upgrading the fixed-time artificial insemination (FTAI) protocol in Romanian buffaloes

Ciornei,

Roşca

2023

Front. Vet. Sci.

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The present study describes the challenges of assisted reproduction in Romanian buffaloes while increasing the efficacy of artificial insemination by choosing the most suitable method. The modified fixed-time artificial insemination (FTAI) protocol with sexed semen was used to increase the conception rate. This study included a total of 80 buffalo heifers that received ovarian stimulation using the OvSynch protocol. Two groups (n = 40), namely, a control group, in which the classic FTAI method was performed, and an experimental group, in which deep intrauterine AI was performed in cows that had developed a dominant follicle (US+UcFTAI), were randomly selected. The conception rate (CR) was 63.6% in the experimental group, which was statistically higher (P < 0.05) than the control group (30%). The ultrasound examination indicated that, using the OvSynch protocol, 82.5% (33 out of 40) of buffaloes developed a dominant follicle (DF) while the distribution between the warm and cold seasons was 75 and 90%, respectively. The CR was 60% during the hot season and 66.6% during the cold season. At calving, 92.5% female fetuses were born. The improved FTAI method in this study enhanced the results by reducing the waste of sexed semen and maximizing the response to OvSynch, making it a recommendation for practitioners. This study presents preliminary results and highlights that genetic progress is difficult to achieve. A systematic approach is needed in order to choose the most suitable biotechnological method for each farm.

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GPGMH, a New Fixed Timed-AI Synchronization Regimen for Swamp and River Crossbred Buffaloes (Bubalus bubalis)

Cited by 5 publications

References 33 publications

Effect of Different Synchronization Regimens on Reproductive Variables of Crossbred (Swamp × Riverine) Nulliparous and Multiparous Buffaloes during Peak and Low Breeding Seasons

Effect of Different Synchronization Regimens on Reproductive Variables of Crossbred (Swamp × Riverine) Nulliparous and Multiparous Buffaloes during Peak and Low Breeding Seasons

Nasal immunization with AMH-INH-RFRP DNA vaccine for improving follicle development and fertility in buffaloes

Upgrading the fixed-time artificial insemination (FTAI) protocol in Romanian buffaloes

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