GPA1, a haploid-specific essential gene, encodes a yeast homolog of mammalian G protein which may be involved in mating factor signal transduction

ADP ribosylation factor is an essential protein in Saccharomyces cerevisiae and is encoded by two genes.

“…There are two known yeast G,, proteins; GPAI is involved in mating pheromone signal transduction (10,22), whereas the function of GPA2 is unknown, and mutations in GPA2 result in no discernible phenotype (25). If ARF were required for GPAI function, then sterility or constitutive activation of the mating pathway might be expected, but neither of these phenotypes was observed in either arfi null mutants or under conditions of ARF depletion (see Results; also, unpublished observation).…”

Functional analyses of mammalian protein kinase C isozymes in budding yeast and mammalian fibroblasts

“…Plasmids pBC50 (Courchesne et al 1989) and pG1501 (Miyajima et al 1987) carry the structural genes of KSS1 and GPA1, respectively, fused with the GAL1 promoter. These plasmids have URA3 as a selection marker.…”

Search for Protein Farnesyltransferase Inhibitors of Microbial Origin: Our Strategy and Results as well as the Results Obtained by Other Groups

“…In the haploid phase, two cell types, a and a a, each secrets specific mating pheromones, known as a-and a a-factors, respectively. Mating between a-and a a haploid cells result in a third cell type, a/a a diploids [13,14]. The process and the structures of a-and a a-factors [15] are summarized in Fig.…”