Throughout the last decade, the international donor community has developed a plethora of regulatory initiatives for responsible agricultural investments. It remains unclear how such guidelines are invoked in practice in investment cases, and whether their use can prevent conflict and protect local land rights, as promoted. Uncovering how international guidelines work necessitates an understanding of the formallegal setting and underlying land tenure regimes that shape investment projects. In Uganda, these contexts vary from region to region and investments take place on land held under various tenure regimes, including private, state-owned, and customary land. Based on 8 months of fieldwork in Uganda, I compare three cases of largescale land investments in different settings and argue that variation in the underlying land tenure systems determines the variation, uneven applicability and effectiveness of global governance mechanisms.