Gorkha (Nepal) 2015

Rajendran, C. P.; Rajendran, Kusala

doi:10.1007/978-981-16-4748-2_12

Cited by 1 publication

(2 citation statements)

References 53 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…They proposed an effect called “attinebility”, which describes the increased rebinding probability of an analyte if the neighboring receptor density is higher. This phenomenon results in a higher apparent affinity of the analyte, despite being bound to only one receptor at a time . These examples demonstrate that the original Langmuirian isotherm sometimes is unable to model biosensing data correctly and that there is a need for better models.…”

Section: Nonspecific Binding As a Bottleneck In Diagnostically Releva...mentioning

confidence: 98%

“…Several groups have observed non-Langmuirian receptor density-dependent DNA–DNA interactions. , The observed effect is non-Langmuirian because the fractional occupancy of DNA was increased by increasing the receptor DNA density. Ozer et al accounted for this effect by implementing a cooperative model, which combines the Langmuirian specific binding with a non-Langmuirian receptor density-dependent cooperative NSB .…”

Section: Nonspecific Binding As a Bottleneck In Diagnostically Releva...mentioning

confidence: 99%

See 1 more Smart Citation

Nonspecific Binding—Fundamental Concepts and Consequences for Biosensing Applications

et al. 2021

View full text Add to dashboard Cite

Nature achieves differentiation of specific and nonspecific binding in molecular interactions through precise control of biomolecules in space and time. Artificial systems such as biosensors that rely on distinguishing specific molecular binding events in a sea of nonspecific interactions have struggled to overcome this issue. Despite the numerous technological advancements in biosensor technologies, nonspecific binding has remained a critical bottleneck due to the lack of a fundamental understanding of the phenomenon. To date, the identity, cause, and influence of nonspecific binding remain topics of debate within the scientific community. In this review, we discuss the evolution of the concept of nonspecific binding over the past five decades based upon the thermodynamic, intermolecular, and structural perspectives to provide classification frameworks for biomolecular interactions. Further, we introduce various theoretical models that predict the expected behavior of biosensors in physiologically relevant environments to calculate the theoretical detection limit and to optimize sensor performance. We conclude by discussing existing practical approaches to tackle the nonspecific binding challenge in vitro for biosensing platforms and how we can both address and harness nonspecific interactions for in vivo systems.

show abstract

Section: Nonspecific Binding As a Bottleneck In Diagnostically Releva...mentioning

confidence: 98%

Section: Nonspecific Binding As a Bottleneck In Diagnostically Releva...mentioning

confidence: 99%

Nonspecific Binding—Fundamental Concepts and Consequences for Biosensing Applications

et al. 2021

View full text Add to dashboard Cite

show abstract

Gorkha (Nepal) 2015

Cited by 1 publication

References 53 publications

Nonspecific Binding—Fundamental Concepts and Consequences for Biosensing Applications

Nonspecific Binding—Fundamental Concepts and Consequences for Biosensing Applications

Contact Info

Product

Resources

About