Gordonia polyisoprenivorans from groundwater contaminated with landfill leachate in a subtropical area: characterization of the isolate and exopolysaccharide production

Abstract

A strain of Gordonia sp. (strain Lc), from landfill leachate-contaminated groundwater was characterized by polyphasic taxonomy and studied for exopolysaccharide (EPS) production. The cells were Gram-positive, catalase-positive, oxidase-negative and non-motile. The organism grew both aerobically and, in anoxic environment, in the presence of NaNO3. Rods occured singly, in pairs or in a typical coryneform V-shaped. The organism had morphological, physiological and chemical properties consistent with its assignment to the genus Gordonia and mycolic and fatty acid pattern that corresponded to those of G. polyisoprenivorans DSM 44302 T . The comparison of the sequence of the first 500 bases of the 16S rDNA of strain Lc gave 100% similarity with the type strain of Gordonia polyisoprenivorans DSM 44302 T . Experiments conducted in anaerobic conditions in liquid E medium with either glucose or sucrose as the main carbon source showed that sucrose did not support the growth of Lc strain and that on glucose the maximum specific growth rate was 0.17h -1 , representing a generation time of approximately 4 hours. On glucose, a maximum of total EPS was produced during the exponential phase (126.17 ± 15.63 g l -1 ). The production of free EPS exceeded that of capsular and the free/capsular EPS ratio increased from 1.9 during the exponential phase to 7.8 during the stationary phase. At present, six strains of G. polyisoprenivorans have been isolated from various environments. Lc is the sixth strain of G. polyisoprenivorans described, the second strain detected in the landfill leachatecontaminated groundwater and the first that is being studied for EPS production.

Isolation and application of Gordonia sp. JC11 for removal of boat lubricants

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Development of an electroactive aerobic biocathode for microbial fuel cell applications

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Culture and exopolysaccharide production from sugarcane molasses by Gordonia polyisoprenivorans CCT 7137, isolated from contaminated groundwater in Brazil

“…The amount of total EPS produced by G. polyisoprenivorans CCT 7137 was higher than that observed by Fusconi et al (2006) in the culture of G. polyisoprenivorans CCT 7277 in E medium containing glucose. The latter produced a maximum of 126.17 g L -1 during the stationary phase.…”