Gordonia mangrovi sp. nov., a novel actinobacterium isolated from mangrove soil in Hainan

Abstract

A novel actinobacterium, designated strain HNM0687T, was isolated from mangrove soil samples collected from Hainan Province, PR China and its polyphasic taxonomy was studied. Based on the results of 16S rRNA gene sequence analysis, strain HNM0687T was closely related to Gordonia bronchialis NBRC 16047T (98.7 %), Gordonia rhizosphera NBRC 16068T (98.2 %), Gordonia oryzae RS15-1ST (97.9 %), Gordonia polyisoprenivorans NBRC 16320T (97.7 %) and Gordonia sediminis AMA 120T (97.7 %). Genome-based comparisons revealed a clear distinction in average nucleotide identity values between strain HNM0687T and its closely related strains (74.4–78.3 %). Strain HNM0687T contained meso-diaminopimelic acid, arabinose and galactose in whole-cell hydrolysates. Mycolic acid was present. The menaquinones of strain HNM0687T were MK-9(H2) and MK-7(H2). The phospholipids of the isolate were composed of diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylinositol. The major fatty acids were C16 : 0, C16 : 1 ω7c/C16 : 1 ω6c, C18 : 010-methyl (TBSA), C18 : 0 and C18 : 1 ω9c. Based on its genotypic, chemotaxonomic and phenotypic characteristics, it is concluded that strain HNM0687T represents a novel species of the genus Gordonia for which the name Gordonia mangrovi sp. nov. is proposed. The type strain is HNM0687T (=CCTCC AA 2019074 T=KCTC 49383 T).

Gordonia tangerina sp. nov., isolated from seawater

“…The only quinone in strain GW1C4-4 T was MK-9 (H 2 ), which was also the predominant quinone in most of the species in the genus Gordonia. Compared to strain GW1C4-4 T , the two reference type strains also contained other kinds of quinone such as MK-7 (H 2 ) and MK-8 (H 2 ) [15]. For the analysis of cellular fatty acids profiles, the cell biomass of strain GW1C4-4 T and reference strains G. bronchialis DSM 43247 T and G. mangrovi HNM0687 T were collected by cultivating on MB plates at 28 °C for 48 h. Cellular fatty acids were determined through the protocol of Sherlock Microbial Identification System (midi) version 6.0B.…”

“…S6). In comparison, the polar lipids of G. mangrovi HNM0687 T were diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylinositol [15], and the polar lipids of G. bronchialis DSM 43247 T were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol and phosphatidylinositol mannosides [2]. The respiratory quinone was analysed by high-performance liquid chromatography-mass spectrometry [49].…”

“…The only quinone in strain GW1C4-4 T was MK-9 (H 2 ), which was also the predominant quinone in most of the species in the genus Gordonia . Compared to strain GW1C4-4 T , the two reference type strains also contained other kinds of quinone such as MK-7 (H 2 ) and MK-8 (H 2 ) [15]. For the analysis of cellular fatty acids profiles, the cell biomass of strain GW1C4-4 T and reference strains G.…”

“…Members in the genus were isolated from diverse matrices. Most of them were isolated from sputum samples from patients with pulmonary disease [2][3][4][5][6], soil and sediment [7][8][9][10][11][12][13][14][15][16][17][18][19][20], sludge and wastewater bioreactor from wastewater treatment plants [21][22][23][24][25][26][27][28][29], and animals [30,31]. Gordonia species have a great capacity to degrade hydrocarbons, recalcitrant pollutants and natural compounds that are hard to biodegrade [32], such as oil [33], phthalic acid esters [34], polycyclic aromatic hydrocarbons [35] and rubber [36].…”

Gordonia pseudamarae sp. nov., a home for novel actinobacteria isolated from stable foams on activated sludge wastewater treatment plants

“…There is also evidence that they have the potential to produce bioactive and antimicrobial agents [20]. Gordonia species are widely distributed in natural habitats [19,21,22] and are associated with diverse invertebrates and vertebrates from marine and terrestrial sources [14,19]. They have also been implicated as opportunistic pathogens of humans [1,23,24].…”