Gordonia caeni sp. nov., isolated from sludge of a sewage disposal plant

Abstract

A Gram-stain-positive, strictly aerobic, short-rod-shaped, non-motile strain (designated MJ32 T ) was isolated from a sludge sample of the Daejeon sewage disposal plant in South Korea. A polyphasic approach was applied to study the taxonomic position of strain MJ32T . Strain MJ32 T showed highest 16S rRNA gene sequence similarity to Gordonia hirsuta DSM 44140 T (98.1 %)and Gordonia hydrophobica DSM 44015 T (97.0 %); levels of sequence similarity to the type strains of other recognized Gordonia species were less than 97.0 %. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain MJ32 T belonged to the clade formed by members of the genus Gordonia in the family Gordoniaceae. The G+C content of the genomic DNA of strain MJ32 T was 69.2 mol%. Chemotaxonomically, strain MJ32 T showed features typical of the genus Gordonia. The predominant respiratory quinone was MK-9(H 2 ), the mycolic acids present had C 56 -C 60 carbon atoms, and the major fatty acids were C 16 : 0 (34.6 %), tuberculostearic acid (21.8 %), C 16 : 1 v7c (19.5 %) and C 18 : 1 v9c (12.7 %). The peptidoglycan type was based on meso-2,6-diaminopimelic acid as the diagnostic diamino acid with glycolated sugars. On the basis of phylogenetic inference, fatty acid profile and other phenotypic properties, strain MJ32

Gordonia tangerina sp. nov., isolated from seawater

“…Members in the genus were isolated from diverse matrices. Most of them were isolated from sputum samples from patients with pulmonary disease [2][3][4][5][6], soil and sediment [7][8][9][10][11][12][13][14][15][16][17][18][19][20], sludge and wastewater bioreactor from wastewater treatment plants [21][22][23][24][25][26][27][28][29], and animals [30,31]. Gordonia species have a great capacity to degrade hydrocarbons, recalcitrant pollutants and natural compounds that are hard to biodegrade [32], such as oil [33], phthalic acid esters [34], polycyclic aromatic hydrocarbons [35] and rubber [36].…”

Gordonia crocea sp. nov. and Gordonia spumicola sp. nov. isolated from sludge of a wastewater treatment plant

“…Gordonia sputi was also isolated from the sputa of patients with chronic pulmonary diseases [5], and about one third of the species of the genus Gordonia described hitherto have been considered as opportunistic pathogens in humans [3]. On the other hand, the remaining two thirds of the species of the genus Gordonia reported have been isolated from various environments [6], and most of them have been reported to play important roles in bioremediation or the biodegradation of pollutants [7][8][9][10][11].…”