Good’s buffers as novel phase-forming components of ionic-liquid-based aqueous biphasic systems

Luís, Ana Teresa; Dinis, Teresa B. V.; Passos, Helena; Taha, Mohamed; Freire, Mara G.

doi:10.1016/j.bej.2015.05.008

Cited by 23 publications

(11 citation statements)

References 43 publications

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“…36 ABS formed by imidazolium-based ILs and organic biological buffers have also been investigated for the extraction of Lphenylalanine and L-tryptophan, leading to extraction efficiencies to the IL-rich phase ranging between 22 and 100%, yet still requiring the use of 30-49 wt% of IL 1 9-36 wt% of biological buffer. 61 These results confirm the efficiency of IL-based ABS as extraction strategies, as well as the potential of AGB-ILs to substitute the largely investigated imidazolium-based ILs. In summary, the AGB-IL-based ABS investigated display a good performance to extract amino acids from aqueous media, with the additional advantage of using lower amounts of salt in the ABS composition and ILs of lower ecotoxicity.…”

Section: Extraction Of Amino Acidssupporting

confidence: 74%

“…Extraction efficiencies for L‐tryptophan ranging between 72% and 99% with ABS composed of imidazolium‐based ILs and potassium citrate (K 3 C 6 H 5 O 7 ) have been reported, yet using imidazolium‐based ILs, a strong salting‐out species, and higher amounts of salt (20 wt%) . ABS formed by imidazolium‐based ILs and organic biological buffers have also been investigated for the extraction of L‐phenylalanine and L‐tryptophan, leading to extraction efficiencies to the IL‐rich phase ranging between 22 and 100%, yet still requiring the use of 30–49 wt% of IL + 9–36 wt% of biological buffer . These results confirm the efficiency of IL‐based ABS as extraction strategies, as well as the potential of AGB‐ILs to substitute the largely investigated imidazolium‐based ILs.…”

Section: Resultsmentioning

confidence: 99%

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Glycine‐betaine ionic liquid analogues as novel phase‐forming components of aqueous biphasic systems

Pereira

Almeida

Gomes

et al. 2018

Biotechnology Progress

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Given the biotechnology advances observed in recent years in terms of upstream, the development of effective downstream processes becomes mandatory to decrease the associated costs of biotechnological-based products. Although a large interest has been devoted to ionic-liquid-based aqueous biphasic systems (IL-based ABS) as tailored separation platforms, imidazolium-based ILs have been the preferred choice as phase-forming agents. To overcome some toxicity and biodegradability issues associated to imidazolium-based ILs, novel ABS composed of ILs analogues of glycine-betaine (AGB-ILs) are here proposed and investigated. Five AGB-ILs were synthesized, characterized in terms of ecotoxicity, and applied toward the development of novel ABS formed with Na SO . Three commercial ILs were also investigated for comparison purposes. The respective ABS ternary phase diagrams, as well as the tie-lines and tie-line lengths, were determined at 25°C. Finally, their performance as extraction strategies was evaluated with five amino acids (L-tryptophan, L-phenylalanine, D-phenylalanine, L-tyrosine and L-3,4-dihydroxyphenylalanine/L-dopa). In all studied systems amino acids preferentially migrate to the IL-rich phase, and with AGB-ILs, the amino acid extraction efficiencies to the IL-rich phase range between 65% and 100%, obtained in a single-step. Furthermore, the studied AGB-ILs display a higher ability to form ABS and to extract amino acids than ABS composed of more traditional and commercial ILs. In summary, novel ABS composed of AGB-ILs can be formed and used as separation routes of value-added compounds of biotechnological interest. © 2018 American Institute of Chemical Engineers Biotechnol. Prog., 2018 © 2018 American Institute of Chemical Engineers Biotechnol. Prog., 34:1205-1212, 2018.

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Section: Extraction Of Amino Acidssupporting

confidence: 74%

Section: Resultsmentioning

confidence: 99%

Glycine‐betaine ionic liquid analogues as novel phase‐forming components of aqueous biphasic systems

Pereira

Almeida

Gomes

et al. 2018

Biotechnology Progress

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“…Alternative ATPS constituted by ionic liquids (ILs) and salts have been proposed to complement ATPS based on polymers [12]. As a result of the ILs diversity in terms of chemical structures, IL-ATPS provide a wider range of polarities between their phases [13,14]. This translates into more efficient, and often more selective, extraction and separation processes.…”

Section: Introductionmentioning

confidence: 99%

Understanding the effect of ionic liquids as adjuvants in the partition of biomolecules in aqueous two-phase systems formed by polymers and weak salting-out agents

Neves

Sousa

Pereira

et al. 2019

Biochemical Engineering Journal

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Ionic liquids (ILs) as adjuvants in polymer-salt aqueous two-phase systems (ATPS) have been used to improve the extraction of biomolecules. However, the impact of ILs as adjuvants on the partition of biomolecules is still poorly understood. Previous works mostly focused on ATPS based on strong salting-out agents, which may mask the IL effect. In this work, ATPS formed by polyethylene glycol (PEG 400) and a weak salting-out salt ((NH4)2SO4) with a wide number of ILs as adjuvants (chloride-based combined with cholinium, imidazolium, pyrrolidinium, piperidinium, tetralkylammonium and tetralkylphosphonium cations) were investigated. The respective phase diagrams were determined, and the systems extraction performance for a wide range of biomolecules (phenolic compounds, alkaloids and amino acids) was investigated. The results obtained show that ILs as adjuvants in polymer-salt ATPS modulate the partition of biomolecules. In particular, more hydrophobic ILs significantly enhance the partition of more hydrophobic biomolecules to the PEG-rich phase (where the IL is enriched). Furthermore, the intensity of the IL effect is more pronounced when using weak salting-out agents. A linear correlation between the biomolecules and the ILs partition coefficients, and with the biomolecules octanol-water partition coefficients, was found. In most ATPS formed by polymers and salts using ILs as adjuvants, the biomolecules partition is driven by the ILs partition and by the difference in hydrophobicity between the coexisting phases.

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“…Only a few studies have addressed the environmental impact or potential risks associated with ILs’ design, use and recycling. Thus, a rigorous selection of ILs and salt families and structures must be carried out beforehand based on the type of target biomolecules to be extracted, in order to make the most sustainable choice [ 14 , 16 , 17 , 18 ]. Imidazolium-based ILs such as [C4Mim]Br − and [C4Mim]Cl − are the most frequently used IL cores and are, therefore, well documented [ 19 , 20 ], hence we chose to study these in association with harmless salts.…”

Section: Introductionmentioning

confidence: 99%

Aqueous Two-Phase System Extraction of Polyketide-Based Fungal Pigments Using Ammonium- or Imidazolium-Based Ionic Liquids for Detection Purpose: A Case Study

Lebeau¹,

Petit²,

Fouillaud³

et al. 2020

JoF

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Demand for microbial colorants is now becoming a competitive research topic for food, cosmetics and pharmaceutics industries. In most applications, the pigments of interest such as polyketide-based red pigments from fungal submerged cultures are extracted by conventional liquid–liquid extraction methods requiring large volumes of various organic solvents and time. To address this question from a different angle, we proposed, here, to investigate the use of three different aqueous two-phase extraction systems using either ammonium- or imidazolium-based ionic liquids. We applied these to four fermentation broths of Talaromyces albobiverticillius (deep red pigment producer), Emericella purpurea (red pigment producer), Paecilomyces marquandii (yellow pigment producer) and Trichoderma harzianum (yellow-brown pigment producer) to investigate their selective extraction abilities towards the detection of polyketide-based pigments. Our findings led us to conclude that (i) these alternative extraction systems using ionic liquids as greener extractant means worked well for this extraction of colored molecules from the fermentation broths of the filamentous fungi investigated here; (ii) tetrabutylammonium bromide, [N4444]Br-, showed the best pigment extraction ability, with a higher putative affinity for azaphilone red pigments; (iii) the back extraction and recovery of the fungal pigments from ionic liquid phases remained the limiting point of the method under our selected conditions for potential industrial applications. Nevertheless, these alternative extraction procedures appeared to be promising ways for the detection of polyketide-based colorants in the submerged cultures of filamentous fungi.

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Good’s buffers as novel phase-forming components of ionic-liquid-based aqueous biphasic systems

Cited by 23 publications

References 43 publications

Glycine‐betaine ionic liquid analogues as novel phase‐forming components of aqueous biphasic systems

Glycine‐betaine ionic liquid analogues as novel phase‐forming components of aqueous biphasic systems

Understanding the effect of ionic liquids as adjuvants in the partition of biomolecules in aqueous two-phase systems formed by polymers and weak salting-out agents

Aqueous Two-Phase System Extraction of Polyketide-Based Fungal Pigments Using Ammonium- or Imidazolium-Based Ionic Liquids for Detection Purpose: A Case Study

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