Gonococcal Complement-fixation Test in Complicated and Uncomplicated Gonorrhoea

Magnusson, Bertil; Kjellander, J

doi:10.1136/sti.41.2.127

Cited by 20 publications

(27 citation statements)

References 4 publications

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“…Buchanan, Swanson, Holmes, Kraus, and Gotschlich Our observation that more females than males infected with gonococci have elevated antibody levels to gonococcal pili is consistent with nearly every previous report of serologic tests for gonorrhea (27,30,40,(43)(44)(45)(46)48) and may be related to the duration of infection in each sex. Most men develop dysuria and urethral discharge within 5 days after infection and seek diagnosis or treatment.…”

supporting

confidence: 76%

“…1 and 3) developed elevated levels within the first week of illness. Magnusson and Kjellander (27), Chacko, and Nair (50), and Genner, Anton, and Boas (62) also found that elevations in antibody were detectable after 7-10 days of illness. Hess, Hunter, and Ziff, using an indirect fluorescent antibody method, observed antibody to gonococci in 80% of sera obtained from patients during the first week of arthritis, and in 100% of sera obtained 8-21 days after the arthritis began (63).…”

Section: Introductionmentioning

confidence: 96%

“…Hess, Hunter, and Ziff, using an indirect fluorescent antibody method, observed antibody to gonococci in 80% of sera obtained from patients during the first week of arthritis, and in 100% of sera obtained 8-21 days after the arthritis began (63). The time required for antibody to decline to normal levels following treatment was found by Hess' to be 1-9 mo (63), and by Magnusson and Kjellander to be 1 mo to 1 yr (27). Genner observed elevated antibody levels for 6 mo to 2 yr in untreated patients (62).…”

Section: Introductionmentioning

confidence: 99%

“…Magnusson and Kjellander used a suspension of gonococci heated to 60°as antigens in a complement fixation test (27). Watt, Ward, and Glynn (30) and Ratnatunga (43) also used heated whole organisms in a complement-fixation test system.…”

Section: Introductionmentioning

confidence: 99%

“…8). Culture results negative (45) positive (58 (27)(28)(29)(30)(31)(32)(33)(34)(35)(36)(37)(38)(39)(40)(43)(44)(45)(46)(47)(48)(49)(50)(51)(52)(53)(54). The use of a pure antigen preparation decreases the chance of detecting crossreacting antibody responses to organisms other than gonococci, and is a prerequisite to quantitating the antibody response to a single antigen.…”

Section: Introductionmentioning

confidence: 99%

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Quantitative Determination of Antibody to Gonococcal Pili. CHANGES IN ANTIBODY LEVELS WITH GONOCOCCAL INFECTION

Buchanan¹,

Swanson²,

Holmes³

et al. 1973

J. Clin. Invest.

106

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A B S T R A C T Gonococcal pili, pure by the criteria of electron microscopic examination and polyacrylamide gel electrophoresis in sodium dodecyl sulfate, have been prepared by repeated cycles of precipitation with 0.1 M MgCli, followed by dissolution in 0.01 M Tris pH 8, 0.01 M NaNs. Using a fluorescein-conjugated antibody prepared to pili from a single strain, pilar antigen(s) was found to be present in each of 18 strains of gonococci tested, and absent from strains of pilated meningococci, nonpathogenic Neisseria sp., and Escherichia coli. Purified pili, labeled with 'I were used in an antigen binding assay to quantitatively measure antibody to pili in rabbit sera and in 561 human sera. The range of antibody activity for 133 persons unlikely to have experienced gonorrhea was 0.1-1.6 /Ag/ml with a geometric mean of 0.5 ,ug/ml. This geometric mean antibody activity was significantly lower than the geometric mean for asymptomatically infected males (1.0 isg/ml, P < 0.002), males with symptomatic gonococcal anterior urethritis (1.6 ug/ml, P <0.001), or asymptomatically infected females (4.2 Ag/ml, P<0.001). Antibody appeared in elevated levels (> 1.6 Lg/ml) 2-3 wk after infection and returned toward control levels 1 or more months after treatment. Antibody levels higher than 1.6 Ag/ml were found in 26 (50%) of 52 males with gonococcal anterior urethritis, in 10 (33%) of of 56 asymptomatically infected females. In a high-risk group of 103 females for whom culture results and antibody to pili were compared, 58 (57%) had elevated antibody levels to pili and 86% of the infected females were within this seropositive group. The antigen binding assay may provide a means to detect asymptomatic gonococcal infection in women.

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supporting

confidence: 76%

Section: Introductionmentioning

confidence: 96%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Quantitative Determination of Antibody to Gonococcal Pili. CHANGES IN ANTIBODY LEVELS WITH GONOCOCCAL INFECTION

Buchanan¹,

Swanson²,

Holmes³

et al. 1973

J. Clin. Invest.

106

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Quantitation of Neisseria gonorrhoeae from Women with Gonorrhea

Lowe

Kraus

1976

Journal of Infectious Diseases

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The number of infecting organisms has been shown to be an important variable in animal models of infection with Neisseria gonorrhoeae. This variable may also be important in the natural transmission of gonorrhea in humans. The number of gonococci in the cervicovaginal area of women with gonorrhea is unknown, as are the effects of certain physiologic or therapeutic variables on the number of gonococci. In this study a semiquantitative technique for the enumeration of N. gonorrhoeae was used; 10 ml of phosphate-buffered saline was directed against the cervix and vaginal wall, and the number of colony-forming units (cfu) of N. gonorrhoeae in the aspirate was determined. The number of N. gonorrhoeae recovered ranged from 4.0 X 10(2) to 1.8 X 10(7) cfu. The geometric mean number was 1.45 X 10(5) cfu, with a standard deviation of 1.04 X 10(1) cfu. Statistical analyses showed that the number of gonococci was not influenced by the use of oral contraceptives, concurrent infection with Trichomonas vaginalis and/or Corynebacterium (Haemophilus)vaginalis, time of douche prior to examination, or phase of the menstrual cycle at which the women were examined. The results indicated a wide range in the number of N. gonorrhoeae recoverable by vaginal irrigation but failed to define the reason for this variability.

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Quantitation of Serum Antibodies to Surface Antigens of Neisseria gonorrhoeae with Radiolabeled Protein A of Staphylococcus aureus

Christensen

Mårdh

et al. 1976

Journal of Infectious Diseases

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Antibodies in human sera against surface antigens of Neisseria gonorrhoeae were detected with use of 125I-labeled protein A from Staphylococcus aureus. Serum was allowed to react with a suspension of whole gonococci, and the antibodies attached to the bacteria were quantitated with protein A, which reacts with the Fc fragment of IgG. Tests with five human sera with a gonococcoal complement-fixation titer of greater than or equal to 1:30 revealed no difference between use of freshly isolated gonococci and use of strains subcultured daily on artificial medium for 10 years. Antibodies cross-reacting with N. gonorrhoeae and Neisseria meningitidis were found in human sera. The results of the test with labeled protein A varied with the serum titer of complement-fixing antibody to N. gonorrhoeae. Acute and convalescent sera from six of seven patients, including one with disseminated gonococcal infection, showed significantly larger differences in antibodies to N. gonorrhoeae than did sera of women without evidence of genital infection. Complement-fixation titers changed significantly in only three of the six patients.

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Gonococcal Complement-fixation Test in Complicated and Uncomplicated Gonorrhoea

Cited by 20 publications

References 4 publications

Quantitative Determination of Antibody to Gonococcal Pili. CHANGES IN ANTIBODY LEVELS WITH GONOCOCCAL INFECTION

Quantitative Determination of Antibody to Gonococcal Pili. CHANGES IN ANTIBODY LEVELS WITH GONOCOCCAL INFECTION

Quantitation of Neisseria gonorrhoeae from Women with Gonorrhea

Quantitation of Serum Antibodies to Surface Antigens of Neisseria gonorrhoeae with Radiolabeled Protein A of Staphylococcus aureus

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