Gonadotropins in European sea bass: Endocrine roles and biotechnological applications

Mazón, Marı́a J.; Molés, Gregorio; Rocha, Ana; Crespo, Berta; Lan-Chow-Wing, Olivier; Espigares, F.; Muñoz, Iciar; Felip, Alicia; Carrillo, Manuel; Zanuy, Silvia; Gómez, Ana

doi:10.1016/j.ygcen.2015.05.002

Cited by 28 publications

(25 citation statements)

References 137 publications

(135 reference statements)

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“…Consistent with the gene expression profiles, the serum levels of 11-KT and E2 were significantly increased compared to the control fish, but decreased after 10 weeks compared to the 6-week timepoint in the treatment group. Similarly, recombinant or affinity-purified FSH significantly enhanced the synthesis of 11-KT and E2 in tilapia and rainbow trout ( Oncorhynchus mykiss ) (37, 38). It therefore appears that treatment with high doses of FSH during sex differentiation increased endogenous 11-KT and E2 levels but suppressed the expression of cyp19a1a , thus leading to male fate.…”

Section: Discussionmentioning

confidence: 98%

New Insights Into the Role of Follicle-Stimulating Hormone in Sex Differentiation of the Protogynous Orange-Spotted Grouper, Epinephelus coioides

et al. 2019

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Follicle-stimulating hormone (FSH) signaling is considered to be essential for early gametogenesis in teleosts, but its functional roles during sex differentiation are largely unknown. In this study, we investigated the effects of long-term and short-term FSH injection on sex differentiation in the protogynous orange-spotted grouper ( Epinephelus coioides ). Long-term FSH treatment initially promoted the formation of ovaries but subsequently induced a male fate. The expression of female pathway genes was initially increased but then decreased, whereas the expression of male pathway genes was up-regulated only during long-term FSH treatment. The genes related to the synthesis of sex steroid hormones, as well as serum 11-ketotestosterone and estradiol, were also up-regulated during long-term FSH treatment. Short-term FSH treatment activated genes in the female pathway (especially cyp19a1a ) at low doses but caused inhibition at high doses. Genes in the male pathway were up-regulated by high concentrations of FSH over the short term. Finally, we found that low, but not high, concentrations of FSH treatment activated cyp19a1a promoter activities in human embryonic kidney (HEK) 293 cells. Overall, our data suggested that FSH may induce ovarian differentiation or a change to a male sex fate in the protogynous orange-spotted grouper, and that these processes occurred in an FSH concentration-dependent manner.

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Section: Discussionmentioning

confidence: 98%

New Insights Into the Role of Follicle-Stimulating Hormone in Sex Differentiation of the Protogynous Orange-Spotted Grouper, Epinephelus coioides

et al. 2019

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“…Salmonid studies showed that FSH is regulated during the initial phases of gametogenesis, including vitellogenesis and spermatogenesis, whereas LH dominated during the final maturation process, including oocyte maturation and ovulation in the female and spermiation and milt production in the male (Gen et al, 2003;Jackson, Goldberg, Ofir, Abraham, & Degani, 1999;Ko, Park, Kim, Kobayashi, & Sohn, 2007;Kobayashi, Sohn, Yoshiura, & Aida, 2000;Levavi-Sivan, Bogerd, Mañanós, Gómez, & Lareyre, 2010). This specific pattern had been observed in a number of fish species, such as Senegalese sole (Solea senegalese; Guzmán et al, 2009;Chauvigné et al, 2016), European sea bass (Dicentrarchus labrax; Mateos et al, 2003;Mazón et al, 2015), coho salmon (Oncorhynchus kisutch; Luckenbach, Dickey, & Swanson, 2011), and greater amberjack (Seriola dumerili; Nyuji et al, 2016). Unlike salmonids, multiple spawning fish have reproductively active asynchronous-type ovaries, which make them capable of spawning several times per year (Murua & Saborido-Rey, 2003;Nyuji et al, 2012).…”

Section: Introductionmentioning

confidence: 93%

Ovarian development and changes in the expression of pituitary gonadotropin subunits during the reproductive cycle of female Malaysian river catfish, Hemibagrus nemurus (Valenciennes, 1840), in captivity

et al. 2017

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Summary Determining the expression level of genes encoding the subunit gonadotropic hormones is critical in understanding the reproductive cycle and gonadal development in fish species. The objectives of this study were to monitor changes in the mRNA expression of three gonadotropin (GtH) subunits (α, FSHβ, and LHβ) during gonadal development of the female Malaysian river catfish, Hemibagrus nemurus, in captivity. In the study a quantitative real‐time PCR approach was developed to assess these three mRNA transcript GtH subunit profiles and compare them with ovarian development. The number of transcript encoding GtH hormone, α, FSHβ, and LHβ subunits in pituitary glands of female H. nemurus was measured throughout the reproductive cycle from August 2013 to October 2015, with fish aged 1–27 months. The mRNA expression of GtH subunits increased with ovarian development throughout the reproductive cycle. The FSHβ mRNA level increased during ovarian development, reached its peak in mid‐vitellogenesis, and was reduced during ovulation and spawning, whereas the LHβ mRNA level significantly increased in late vitellogenesis and reached its peak during the ovulation and spawning season (April–May). In α subunit, the mRNA level corresponded to FSHβ and LHβ, with a peak expression during ovulation. The findings suggest that FSH was strongly activated during vitellogenesis, whereas LH was involved at the end of gonadal development process, during ovulation, and in the final oocyte maturation.

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“…Picos de FSH y antimulleriana (AMH) favorecen la maduración temprana de machos (Pfennig et al, 2015) y los picos nocturnos de LH (en oscuridad) permiten la maduración y la espermiación, estos relojes circadianos son dependientes de la melatonina o de receptores de galanina (grhelina) (Martins et al, 2014). La galanina es un neuropéptido reportado para mamíferos como regulador de las funciones reproductivas, nocicepción, aprendizaje, ingesta de alimentos (Carrillo et al, 2015, Mazón et al, 2015.…”

Section: Cambios En El Eje Cerebro Hipófisis Gónadas (Chg)unclassified

“…Así mismo, la ovulación está precedida de cambios hormonales es así como los niveles de 17B estradiol y testosterona se relacionan con la pubertad (Devlin y Nagahama, 2002) e inclusive por la presencia de xenobióticos que pueden modificar el sexo o la pubertad (Luzio et al, 2016) o por manejo de hormonas recombinantes (Mazón et al, 2015).…”

Section: Inicio De La Pubertadunclassified

Determinación, diferenciación sexual y pubertad en peces

2018

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En piscicultura es de gran utilidad conocer los procesos de determinación sexual y los mecanismos que permiten la diferenciación sexual, así como el momento en el cual se alcanza la madurez y los individuos son capaces de reproducirse. Estos tres procesos son muy flexibles en peces y no solo dependen de diferentes cascadas de genes, si no que algunos factores ambientales son condicionantes o desencadenantes de ellos. En consecuencia, esta revisión pretende dar una mirada a los últimos avances sobre el tema, particularmente las interpretaciones científicas de los eventos de maduración sexual y sus mecanismos reguladores.

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Gonadotropins in European sea bass: Endocrine roles and biotechnological applications

Cited by 28 publications

References 137 publications

New Insights Into the Role of Follicle-Stimulating Hormone in Sex Differentiation of the Protogynous Orange-Spotted Grouper, Epinephelus coioides

New Insights Into the Role of Follicle-Stimulating Hormone in Sex Differentiation of the Protogynous Orange-Spotted Grouper, Epinephelus coioides

Ovarian development and changes in the expression of pituitary gonadotropin subunits during the reproductive cycle of female Malaysian river catfish, Hemibagrus nemurus (Valenciennes, 1840), in captivity

Determinación, diferenciación sexual y pubertad en peces

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