Gonadotropin subunits of the characiform  Astyanax altiparanae:  Molecular characterization, spatiotemporal expression and their possible role on female reproductive dysfunction in captivity

Jesus, Lázaro Wender Oliveira de; Bogerd, Jan; Vieceli, Felipe Monteleone; Branco, Giovana Souza; Camargo, Marília P.; Cassel, Mônica; Moreira, Renata Guimarães; Yan, Chao; Borella, Maria Inês

doi:10.1016/j.ygcen.2016.12.004

Cited by 22 publications

(17 citation statements)

References 60 publications

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“…To evaluate gonadotropin and GnRH expression, real-time quantitative polymerase chain reaction (RT-qPCR) was performed. Forward and reverse primers ( Supplementary Table 1) were designed according to specific sequences of A. altiparanae fshb, lhb, elfa1 (Jesus et al, 2017) and gnrh (Chehade et al, unpublished results). Specificity and validation for fshb, lhb and elfa1 primers are provided by Jesus et al (2017) ( Supplementary Table 1).…”

Section: Rna Extraction and Rt-qpcrmentioning

confidence: 99%

“…For these reasons, the species is of substantial commercial importance (Porto-Foresti et al, 2005). It also serves as a common study model in many topics of research (Adolfi et al, 2015;Camargo et al, 2017;Cassel et al, 2017;Chehade et al, 2015;Costa et al, 2014;Gomes et al, 2013;Jesus et al, 2017;Pereira-Santos et al, 2016;Siqueira-Silva et al, 2015;Yasui et al, 2015). In this context, the aim of this study is to evaluate the effects of chicken GnRH2 (cGnRH2) and zebrafish GnIH-3 (zGnIH-3) on A. altiparanae fshb, lhb, gnrh2 and gnrh3 expression using pituitary explants and brain slices from males.…”

Section: Introductionmentioning

confidence: 99%

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Effects of GnRH and the dual regulatory actions of GnIH in the pituitary explants and brain slices of Astyanax altiparanae males

Branco

Melo

Ricci

et al. 2019

General and Comparative Endocrinology

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The pituitary gonadotropins, Fsh (follicle-stimulating hormone) and Lh (luteinizing hormone), regulate testicular development and functions in all vertebrates. At the pituitary, different signaling systems regulate the synthesis and secretion of the gonadotropins, such as the hypothalamic neuropeptides GnRH (gonadotropin-releasing hormone) and GnIH (gonadotropin-inhibitory hormone). While GnRH exerts stimulatory roles, the actions of GnIH remain controversial for many teleost species. Therefore, the aim of this study was to evaluate the in vitro effects of chicken GnRH2 (cGnRH2) and zebrafish GnIH-3 (zGnIH-3) on the male gonadotropin and GnRH system expression using pituitary explants and brain slices from a neotropical species with economical and ecological relevance, Astyanax altiparanae. Our results showed that in males, cGnRH2 increased fshb and lhb mRNA levels in the pituitary explants. Interestingly, zGnIH-3 has no effect on basal gonadotropin expression, however zGnIH-3 decreased the cGnRH2-induced fshb and lhb transcripts in male pituitary explants. In the male brain slices, zGnIH-3 showed stimulatory effects, increasing gnrh2 mRNA levels. Overall, our results suggested that GnIH seems to have dual regulatory actions on gonadotropin and GnRH2 expression of A. altiparanae males. This study provided basic information on endocrine regulation of A. altiparanae reproduction, and the obtained results will expand our knowledge, improving the reproductive management of this economically important freshwater species.

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Section: Rna Extraction and Rt-qpcrmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Effects of GnRH and the dual regulatory actions of GnIH in the pituitary explants and brain slices of Astyanax altiparanae males

Branco

Melo

Ricci

et al. 2019

General and Comparative Endocrinology

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“…cisteínas adyacentes y el primer sitio de N-glicosilación, que puede estar involucrada en el ensamble de la subunidad y su afinidad para unirse al receptor [17].…”

Section: Resultsunclassified

Clonación del cDNA codificante de las subunidades de la gonadotropina luteinizante de Pimelodus grosskopfii

Betancur-López

Flórez

́Brien

et al. 2020

BSAA

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Pimelodus grosskopfii, es un Siluriforme importante en la pesca continental en Colombia y de interés en la acuicultura. La presente investigación tuvo como objetivo clonar y determinar las secuencias codificantes de las subunidades GP-α y LHβ de la gonadotropina luteinizante de esta especie. Para lograr lo anterior, el ARN total de glándulas pituitarias fue extraído y se realizó síntesis de cDNA. Los productos de PCR purificados de GP-α y LHβ fueron ligados en pJET1.2/blunt ® seguido de la transformación de Escherichia coli DH5α ® y posterior secuenciación de fragmentos de interés. El marco abierto de lectura de las subunidades GP-α y LHβ fue de 351 pb (116 aminoácidos) y 423 pb (140 aminoácidos), respectivamente. Se encontró una alta homología (aminoácidos) en la subunidad GP-α con otras familias de Siluriformes (87-99%) y menor homología con órdenes como Perciformes, Salmoniformes y Cypriniformes (66-90%). De igual manera para la subunidad LHβ, la homología (aminoácidos) fue alta al interior del orden Siluriformes (91-97%) y menor con otros ordenes (65-86%). Los resultados obtenidos en el presente estudio ofrecen información básica sobre las secuencias de las gonadotropinas en bagres neotropicales y pueden ser utilizados para el desarrollo de hormonas recombinantes para optimizar el control reproductivo en P. grosskopfii.

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“…One spawned and one unspawned female from each replicate (confirmed by ovarian histology) were randomly chosen approximately 12 hr post resolving dose (at the end of the trial) for ovarian stereological evaluation and the measurement of plasma levels of 17,20β‐P (Brambila‐Souza et al., 2019; Jesus et al., 2017) and PGF 2α (Figueiredo‐Ariki, 2019).…”

Section: Methodsmentioning

confidence: 99%

Reproductive performance of lambari ( Astyanax altiparanae ) in a seminatural system using different protocols

et al. 2020

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Gonadotropin subunits of the characiform Astyanax altiparanae: Molecular characterization, spatiotemporal expression and their possible role on female reproductive dysfunction in captivity

Cited by 22 publications

References 60 publications

Effects of GnRH and the dual regulatory actions of GnIH in the pituitary explants and brain slices of Astyanax altiparanae males

Effects of GnRH and the dual regulatory actions of GnIH in the pituitary explants and brain slices of Astyanax altiparanae males

Clonación del cDNA codificante de las subunidades de la gonadotropina luteinizante de Pimelodus grosskopfii

Reproductive performance of lambari ( Astyanax altiparanae ) in a seminatural system using different protocols

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