Gonadotropin-releasing hormone and adipokinetic hormone/corazonin-related peptide in the female prawn

Suwansa‐ard, Saowaros; Zhao, Min; Thongbuakaew, Tipsuda; Chansela, Piyachat; Ventura, Tomer; Cummins, Scott F.; Sobhon, Prasert

doi:10.1016/j.ygcen.2016.07.008

Cited by 30 publications

(26 citation statements)

References 66 publications

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“…GnRH-like peptides (so named for their structural similarity) have been identified in several protostome groups 21 , 23 . The giant freshwater prawn, Macrobrachium rosenbergii , possesses all four members of the so-called GnRH superfamily of peptides: RPCH, ACP, Crz and GnRH-like peptide 24 , 25 .…”

Section: Introductionmentioning

confidence: 99%

Characterisation and pharmacological analysis of a crustacean G protein-coupled receptor: the red pigment-concentrating hormone receptor of Daphnia pulex

Marco

Verlinden

Broeck

et al. 2017

Sci Rep

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This is the first pharmacological characterisation of a neuropeptide G protein-coupled receptor (GPCR) in a crustacean. We cloned the ORF of the red pigment-concentrating hormone from a German strain of Daphnia pulex (Dappu-RPCH), as well as that of the cognate receptor (Dappu-RPCHR). Dappu-RPCHR has the hallmarks of the rhodopsin superfamily of GPCRs, and is more similar to insect adipokinetic hormone (AKH) receptor sequences than to receptor sequences for AKH/corazonin-like peptide or corazonin. We provide experimental evidence that Dappu-RPCH specifically activates the receptor (EC50 value of 65 pM) in a mammalian cell-based bioluminescence assay. We further characterised the properties of the ligands for the Dappu-RPCHR by investigating the activities of a variety of naturally-occurring peptides (insect AKH and crustacean RPCH peptides). The insect AKHs had lower EC50 values than the crustacean RPCHs. In addition, we tested a series of Dappu-RPCH analogues, where one residue at a time is systematically replaced by an alanine to learn about the relative importance of the termini and side chains for activation. Mainly amino acids in positions 1 to 4 and 8 of Dappu-RPCH appear responsible for effective activation of Dappu-RPCHR. The substitution of Phe4 in Dappu-RPCH had the most damaging effect on its agonistic activity.

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Section: Introductionmentioning

confidence: 99%

Characterisation and pharmacological analysis of a crustacean G protein-coupled receptor: the red pigment-concentrating hormone receptor of Daphnia pulex

Marco

Verlinden

Broeck

et al. 2017

Sci Rep

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“…Various neuropeptides structurally related to GnRHs (Figure 1 ), such as adipokinetic hormone (AKH), corazonin (CRZ), and AKH/CRZ-related peptide (ACP), have also been identified in diverse invertebrates ( 3 – 11 ). As shown in Figure 1 , these peptides share the N-terminal pyro-Glu residue and C-terminal amide and conserve Phe, Trp, or Tyr residue in position 3, Ser or Thr in position 4, and Trp or Tyr in position 7 with a vertebrate GnRH2 (pQHWSHGWYPGa).…”

Section: Introductionmentioning

confidence: 99%

“…As shown in Figure 1 , these peptides share the N-terminal pyro-Glu residue and C-terminal amide and conserve Phe, Trp, or Tyr residue in position 3, Ser or Thr in position 4, and Trp or Tyr in position 7 with a vertebrate GnRH2 (pQHWSHGWYPGa). Furthermore, molecular phylogenetic and phylogenomic analyses of peptide genes have led to the presumption that GnRH, AKH, CRZ, and ACP originated from common ancestors of the Bilateria ( 3 , 7 – 9 ), whereas the four peptides have been shown to exhibit distinct physiological functions including activation of lipid-mobilization by AKHs, stimulation of heart rate by CRZs ( 10 ), and down-regulation of oocyte proliferation and elevation of total hemolymph lipids by ACP ( 11 ) in arthropods. The cognate receptors for these peptides have also been identified in a wide invertebrate species, revealing that all of these receptors belong to the Class A G protein-coupled receptor (GPCR) family ( 5 , 8 , 12 ).…”

Section: Introductionmentioning

confidence: 99%

Invertebrate Gonadotropin-Releasing Hormone-Related Peptides and Their Receptors: An Update

et al. 2017

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Gonadotropin-releasing hormones (GnRHs) play pivotal roles in reproductive functions via the hypothalamus, pituitary, and gonad axis, namely, HPG axis in vertebrates. GnRHs and their receptors (GnRHRs) are likely to be conserved in invertebrate deuterostomes and lophotrochozoans. All vertebrate and urochordate GnRHs are composed of 10 amino acids, whereas protostome, echinoderm, and amphioxus GnRH-like peptides are 11- or 12-residue peptide containing two amino acids after an N-terminal pyro-Glu. In urochordates, Halocynthia roretzi GnRH gene encodes two GnRH peptide sequences, whereas two GnRH genes encode three different GnRH peptides in Ciona intestinalis. These findings indicate the species-specific diversification of GnRHs. Intriguingly, the major signaling pathway for GnRHRs is intracellular Ca2+ mobilization in chordates, echinoderms, and protostomes, whereas Ciona GnRHRs (Ci-GnRHRs) are endowed with multiple GnRHergic cAMP production pathways in a ligand-selective manner. Moreover, the ligand-specific modulation of signal transduction via heterodimerization among Ci-GnRHR paralogs suggests the species-specific development of fine-tuning of gonadal functions in ascidians. Echinoderm GnRH-like peptides show high sequence differences compared to those of protostome counterparts, leading to the difficulty in classification of peptides and receptors. These findings also show both the diversity and conservation of GnRH signaling systems in invertebrates. The lack of the HPG axis in invertebrates indicates that biological functions of GnRHs are not release of gonadotropins in current invertebrates and common ancestors of vertebrates and invertebrates. To date, authentic or putative GnRHRs have been characterized from various echinoderms and protostomes as well as chordates and the mRNAs have been found to be distributed not only reproductive organs but also other tissues. Collectively, these findings further support the notion that invertebrate GnRHs have biological roles other than the regulation of reproductive functions. Moreover, recent molecular phylogenetic analysis suggests that adipokinetic hormone (AKH), corazonin (CRZ), and AKH/CRZ-related peptide (ACP) belong to the GnRH superfamily but has led to the different classifications of these peptides and receptors using different datasets including the number of sequences and structural domains. In this review, we provide current knowledge of, and perspectives in, molecular basis and evolutionary aspects of the GnRH, AKH, CRZ, and ACP.

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“…It was the first invertebrate neuropeptide to be fully characterized (from the eyestalks of Pandalus borealis ) ( 88 ) and is involved in the distribution of pigments in response to environment ( 89 ) and light-dark adaptation ( 90 ). The structural similarity among RPCH, adipokinetic hormone (AKH), corazonin (Crz), adipokinetic hormone/corazonin-related peptide (ACP) and gonadotropin-releasing hormone (GnRH) in various animals resulted in clustering them together into the GnRH-superfamily ( 91 ). In decapod crustaceans, RPCH is synthesized in the XO/SG complex of the eyestalks, but is also expressed in all major parts of the nervous system, where it is suggested to act as a neurotransmitter having dual endocrine and autocrine/paracrine function ( 66 , 92 , 93 ).…”

Section: Red Pigment-concentrating Hormone (Rpch)mentioning

confidence: 99%

Insights on Molecular Mechanisms of Ovarian Development in Decapod Crustacea: Focus on Vitellogenesis-Stimulating Factors and Pathways

2020

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Vitellogenesis in crustaceans is an energy-consuming process. Though the underlying mechanisms of ovarian maturation in decapod Crustacea are still unclear, evidence indicates the process to be regulated by antagonistically-acting inhibitory and stimulating factors specifically originating from X-organ/sinus gland (XO/SG) complex. Among the reported neuromediators, neuropeptides belonging to the crustacean hyperglycemic hormone (CHH)-family have been studied extensively. The structure and dynamics of inhibitory action of vitellogenesis-inhibiting hormone (VIH) on vitellogenesis have been demonstrated in several species. Similarly, the stimulatory effects of other neuropeptides of the CHH-family on crustacean vitellogenesis have also been validated. Advancement in transcriptomic sequencing and comparative genome analysis has led to the discovery of a large number of neuromediators, peptides, and putative peptide receptors having pleiotropic and novel functions in decapod reproduction. Furthermore, differing research strategies have indicated that neurotransmitters and steroid hormones play an integrative role by stimulating neuropeptide secretion, thus demonstrating the complex intertwining of regulatory factors in reproduction. However, the molecular mechanisms by which the combinatorial effect of eyestalk hormones, neuromediators and other factors coordinate to regulate ovarian maturation remain elusive. These multifunctional substances are speculated to control ovarian maturation possibly via the autocrine/paracrine pathway by acting directly on the gonads or by indirectly exerting their stimulatory effects by triggering the release of a putative gonad stimulating factor from the thoracic ganglion. Acting through receptors, they possibly affect levels of cyclic nucleotides (cAMP and cGMP) and Ca 2+ in target tissues leading to the regulation of vitellogenesis. The “stimulatory paradox” effect of eyestalk ablation on ovarian maturation continues to be exploited in commercial aquaculture operations, and is outweighed by the detrimental physiological effects of this procedure. In this regard, the development of efficient alternatives to eyestalk ablation based on scientific knowledge is a necessity. In this article, we focus principally on the signaling pathways of positive neuromediators and other factors regulating crustacean reproduction, providing an overview of their proposed receptor-mediated stimulatory mechanisms, intracellular signaling, and probable interaction with other hormonal signals. Finally, we provide insight into future research directions on crustacean reproduction as well as potential applications of such research to aquaculture technology development.

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Gonadotropin-releasing hormone and adipokinetic hormone/corazonin-related peptide in the female prawn

Cited by 30 publications

References 66 publications

Characterisation and pharmacological analysis of a crustacean G protein-coupled receptor: the red pigment-concentrating hormone receptor of Daphnia pulex

Characterisation and pharmacological analysis of a crustacean G protein-coupled receptor: the red pigment-concentrating hormone receptor of Daphnia pulex

Invertebrate Gonadotropin-Releasing Hormone-Related Peptides and Their Receptors: An Update

Insights on Molecular Mechanisms of Ovarian Development in Decapod Crustacea: Focus on Vitellogenesis-Stimulating Factors and Pathways

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