Gonadotropin and Testosterone hormone’s serum levels and partial deletions in the AZFc region in Iranian oligozoospermia infertile males

Abstract

To investigate the relation of luteinizing hormone (LH), follicle-stimulating hormone (FSH) and Testosterone serum levels with partial deletions in the AZFc region in Iranian oligozoospermia males. Material and methods: thirty infertile oligozoospermia and 52 Iranian fertile men included. The hormonal assays were measured by the Radioimmunoassay (RIA). Multiplex polymerase chain reaction (M-PCR) using eight sequence-tagged site (STS) markers were measured on the Yq11 chromosome. Results: The mean of FSH and LH levels in all oligozoospermia males were higher than fertile men (p < 0.001) and testosterone was lower significantly (p < 0.001). Five patients showed partial deletions in AZFc region (four had gr/gr and one had b2/b3 deletions). Six fertile men showed partial deletions (five gr/gr and one b2/b3) with higher level of FSH, LH in their group (p < 0.05). Conclusion: According to high incidence of partial deletions in the AZFc region among Iranian oligozoospermia males, hormonal assay and molecular screening should be advised before considering for ART treatments.

Gr/gr deletions on Y-chromosome correlate with male infertility: an original study, meta-analyses and trial sequential analyses

“…Since Europe is largely inhabited by the Caucasian people, similar results in Caucasians and Europe suggest a significant impact of these deletions on male fertility in Caucasians. The lack of a significant association in TSA on Europeans may be due to a relatively smaller number of studies (four Caucasian populations were not European) 19 30 67 74 and higher (I 2 = 71.64%) heterogeneity in the European sub-group in comparison to the Caucasian sub-group (I 2 = 26.83%). Stouffs et al , (2011) 46 also had reported a significant association of the gr/gr deletions with male infertility risk in the European region (OR = 3.05, 95% CI = 1.33–7.03).…”

“…Out of these, fourteen studies were excluded because of the following reasons: two studies were meta-analyses 45 46 , three had recruited participants from the general population 15 31 47 , parts of five studies had been published in other studies 23 48 49 50 51 , one study had irrelevant data 52 and three studies had irrelevant study design 21 27 53 . After including the present case-control study, meta-analyses were performed on twenty-nine studies 14 18 19 20 22 28 30 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 70 71 72 73 74 ( Fig. 2 and supplementary Table S3 ).…”

“…This meta-analysis included twenty-nine studies consisting of 10948 cases and 6604 controls ( supplementary Table S4 ). Five studies had less than a hundred samples in either of the study groups 56 58 66 67 69 . Eleven studies, including the present study, showed a significant association between the gr/gr deletions and male infertility 14 18 19 20 22 30 54 61 68 while others showed no association.…”

Analysis of Partial AZFc (gr/gr, b1/b3, and b2/b3) Deletions in Iranian Oligozoospermia Male Patients as The Candidates of Intra-Cytoplasmic Sperm Injection (ICSI)

“…However, other authors reported that the classical AZFb deletions were more common than the AZFc region in the patients (12,13).…”

Assessment of Steroid 5 Alpha Reductase Enzyme Levels and Its Correlation with Sex Hormones in Infertile Iraqi Men

“…Human infertility is considered as a major health problem, it induces the incapability of marriages who are unfertilized from unprotected sexual correlation at least after 12 months of pregnancy. Impact of infertility on each male and female, making a reference to the word Infertility, in females is complete lack of ability for pregnancy (1). Male infertility is a serious disease involving environmental as well as genetic factors.…”