Gonadotropin and sf-1 regulation of cyp19a1a gene and aromatase activity during oocyte development in the rohu, L. rohita

Moulik, Sujata Roy; Pal, Puja; Majumder, Suravi; Mallick, B.; Gupta, Shreyasi; Guha, Payel; Roy, Sibsankar; Mukherjee, Dilip

doi:10.1016/j.cbpa.2016.02.004

Cited by 22 publications

(19 citation statements)

References 65 publications

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“…In the present study, mRNA transcripts of cyp19a1a and of cyp19a1b were detected in ovary and brain respectively of reproductively mature female perch, A. testudineus . Findings thus corroborate earlier studies on the presence of two distinct cyp19a1 gene in most teleost fish (Barney et al., ; Chang et al., ; Goto‐Kazeto et al., ; Liu et al., ; Roy Moulik et al., ; Trant et al., ). The predicted gene and amino acid sequences of the partially cloned ovary‐ and brain‐derived cyp19a1a and cyp19a1b mRNAs displayed a high degree of identity with those reported for other teleosts.…”

Section: Discussionsupporting

confidence: 90%

“…Aromatase activity in brain and ovary was determined by estimating in vitro conversion of [ 3 H] testosterone to [ 3 H] E2 following the method of Chan and Tan, (1986) as used in our laboratory (Das & Mukherjee, 2013;Roy Moulik et al, 2016). For this, minced tissues were incubated with 140 pmol [ 3 H] testosterone (1 × 10 6 cpm, specific activity 95.0 Ci/mmol) for 6 h. At the end of incubation, tissues were separated from media and a portion of tissues from individual incubation were processed for protein estimation (Lowry et al, 1951).…”

Section: Aromatase Activity Determinationmentioning

confidence: 99%

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RETRACTED: Estrogen‐regulated expression of P450arom genes in the brain and ovaries of adult female Indian climbing perch, Anabas testudineus

et al. 2018

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Cytochrome P450arom (CYP19), a product of cyp19a1 gene, catalyzes the conversion of androgens to estrogens and is essential for regulation of reproductive function in vertebrates. In the present study, we isolated partial cDNA encoding the ovarian (cyp19a1a) and brain (cyp19a1b) P450arom genes from adult female perch, Anabas testudineus and investigated their regulation by estrogen in vivo. Results demonstrated that cyp19a1a and cyp19a1b predominate in ovary and brain respectively, with quantity of both attuned to reproductive cycle. To elucidate estrogen-regulated expression of cyp19a1b in brain and cyp19a1a in ovary, dose- and time-dependent studies were conducted with estrogen in vitellogenic-stage fish in the presence or absence of specific aromatase inhibitor fadrozole. Results demonstrated that treatment of fish with 17β-estradiol (E2; 1.0 μM)) for 6 days caused significant upregulation of cyp19a1b transcripts, aromatase B protein, and aromatase activity in brain in a dose- and time-dependent manner. Ovarian cyp19a1a mRNA, aromatase protein, and aromatase activity, however, was less responsive to E2 than brain. Treatment of fish with an aromatase inhibitor fadrozole for 6 days attenuated both brain and ovarian cyp19a1 mRNAs expression and stimulatory effects of E2 was also significantly reduced. These results indicate that expression of cyp19a1b in brain and cyp19a1a in ovary of adult female A. testudineus was closely associated to plasma E2 levels and seasonal reproductive cycle. Results further show apparent differential regulation of cyp19a1a and cyp19a1b expression by E2/fadrozole manipulation.

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Section: Discussionsupporting

confidence: 90%

Section: Aromatase Activity Determinationmentioning

confidence: 99%

RETRACTED: Estrogen‐regulated expression of P450arom genes in the brain and ovaries of adult female Indian climbing perch, Anabas testudineus

et al. 2018

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“…Apart from eel, most of the teleost fish studied so far possess two aromatase genes, i.e. cyp19a1a and cyp19a1b, due to a duplication event in the actinopterygian lineage (Tchoudakova and Callard 1998, Tong and Chung 2003, Blazquez and Piferrer 2004, Zhang et al 2014, Chaube et al 2015, Roy Moulik et al 2016. The cyp19a1a gene, also known as "gonadal aromatase", is mainly expressed in the differentiating gonad and later in the ovaries and plays a key role in sex differentiation and reproduction in fish (Guiguen et al 2010).…”

Section: Introductionmentioning

confidence: 99%

Refinement of an OECD test guideline for evaluating the effects of endocrine disrupting chemicals on aromatase gene expression and reproduction using novel transgenic cyp19a1a-eGFP zebrafish

et al. 2020

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HAL is a multi-disciplinary open access archive for the deposit and dissemination of scientific research documents, whether they are published or not. The documents may come from teaching and research institutions in France or abroad, or from public or private research centers. L'archive ouverte pluridisciplinaire HAL, est destinée au dépôt et à la diffusion de documents scientifiques de niveau recherche, publiés ou non, émanant des établissements d'enseignement et de recherche français ou étrangers, des laboratoires publics ou privés.Distributed under a Creative Commons Attribution -NonCommercial -NoDerivatives| 4.0International License

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“…The interplay between Nr5a1 and Cyp19a1 can regulate the development and function of the ovary (Gurates et al, 2003;Roy Moulik et al, 2016). In the current research, we showed that miR-1385p directly targets Nr5a1.…”

Section: Mir-138-5p Directly Targets Nr5a1 In Mpgcsmentioning

confidence: 49%

“…Nr5a1 enhances 17β‐estradiol levels by binding to key aromatase Cyp19a1 promoters to activate Cyp19a1 gene expression, which subsequently promotes estrogen synthesis (Lai et al, ; Wang & Gong, ). The interplay between Nr5a1 and Cyp19a1 can regulate the development and function of the ovary (Gurates et al, ; Roy Moulik et al, ). In the current research, we showed that miR‐1385p directly targets Nr5a1.…”

Section: Discussionmentioning

confidence: 99%

LncRNA Gm2044 promotes 17β‐estradiol synthesis in mpGCs by acting as miR‐138‐5p sponge

Ren

et al. 2019

Molecular Reproduction Devel

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Long noncoding RNAs (lncRNAs) have been demonstrated to play vital roles in mammalian reproduction. Our previous research revealed that lncRNA Gm2044 is highly expressed in mouse spermatocytes and regulates male germ cell function. The gene annotation database BioGPS shows that Gm2044 is not only highly expressed in testicular tissue but also in ovarian tissue, which suggests that Gm2044 may be involved in female reproductive development. In this study, we confirmed that lncRNA Gm2044 promotes 17β‐estradiol synthesis in mouse pre‐antral follicular granulosa cells (mpGCs). Furthermore, bioinformatics methods, western blot, and the luciferase assay proved that Gm2044 functions as a miR‐138‐5p sponge to inhibit the direct target of miR‐138‐5p, Nr5a1, which enhances 17β‐estradiol synthesis through cyp19a1 activation. Taken together, our results provide an insight into the mechanistic roles of lncRNA Gm2044 for 17β‐estradiol synthesis by acting as competing‐endogenous RNAs to modulate the function of mpGCs. Studying the potential lncRNAs, which regulate estradiol release, will be beneficial for the diagnosis and treatment of steroid hormone‐related disease.

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Gonadotropin and sf-1 regulation of cyp19a1a gene and aromatase activity during oocyte development in the rohu, L. rohita

Cited by 22 publications

References 65 publications

RETRACTED: Estrogen‐regulated expression of P450arom genes in the brain and ovaries of adult female Indian climbing perch, Anabas testudineus

RETRACTED: Estrogen‐regulated expression of P450arom genes in the brain and ovaries of adult female Indian climbing perch, Anabas testudineus

Refinement of an OECD test guideline for evaluating the effects of endocrine disrupting chemicals on aromatase gene expression and reproduction using novel transgenic cyp19a1a-eGFP zebrafish

LncRNA Gm2044 promotes 17β‐estradiol synthesis in mpGCs by acting as miR‐138‐5p sponge

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