Gonadotropic Activation of Preovulatory Synthesis and Release of Progestin in the Rabbit

Hilliard, Jessamine; Archibald, Donna; Sawyer, Charles H.

doi:10.1210/endo-72-1-59

Cited by 118 publications

(37 citation statements)

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“…The initial increase in luteal 20a-hydroxypregn-4-en-3-one content and concen¬ tration in LH-treated animals of this study is similar to the reported effect of lh on rabbit ovarian interstitial progestin secretion (Hilliard, Archibald & Sawyer, 1963). It has been suggested that ovarian follicular secretion of oestrogen is necessary for luteal maintenance in the rabbit and that ovulation induced by the injection of lh interrupts oestrogen secretion, resulting in regression of cl (Keyes & Nalbandov, 1968).…”

supporting

confidence: 86%

Effect of Luteinizing Hormone on Progestin Levels in Rabbit Corpora Lutea

Kelley¹,

Stormshak²

1969

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(Storm¬ shak & Casida, 1965). Rabbits in the remaining three groups were injected on Day 9 with saline. Autopsies were carried out on treated and control rabbits i, 24 or 48 hr after injection and mature cl were dissected from the ovary, weighed and stored in 95% ethanol.cl were analysed for progesterone and 20oc-hydroxypregn-4-en-3-one. Labelled steroid (progesterone-4-14C, 20a-hydroxypregn-4-en-3-one-l,2-3H; New England Nuclear Corp.) was added to the samples to correct for losses incurred during extraction and purification. Luteal tissue was homogenized in 95% ethanol and filtered. The filtrate was dried under vacuum and further purified by column and paper chromatography as described previously (Stormshak, Inskeep, Lynn, Pope & Casida, 1963). Progesterone and 20a-hydroxypregn-4-en-3-one were separated by paper chromatography with the 171

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supporting

confidence: 86%

Effect of Luteinizing Hormone on Progestin Levels in Rabbit Corpora Lutea

Kelley¹,

Stormshak²

1969

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“…This is considerably more than the amount of a similar Armour lh required for non-primed mature New Zealand does (Hilliard, Archibald & Sawyer, 1963) or more than the amount of highly purified lh required to ovulate pseudopregnant New Zealand does (Stormshak & Casida, 1964 (Foster, Foster & Hisaw, 1937;Foster & Fevold, 1938) suggests that high levels of lh may be less conducive to ovulation. Wilson & Zarrow (1962) also observed a slight downward trend in ovulatory response when high levels of hcg were given to rats and mice.…”

Section: Discussionmentioning

confidence: 95%

“…Many of these vary considerably in potency and a wide range of dosages has been employed. Stormshak & Casida (1964) induced ovulation in mature pseudopregnant New Zealand rabbits with as little as 0-010 mg of purified lh, and Hilliard, Archibald & Sawyer (1963), using pituitary luteinizing hormone (plh), induced ovulation in nonprimed New Zealand does with 0-06 mg of hormone/kg of body weight. With Dutch-belted females 0-5 mg of LH/kg of body weight was as effective as 1-0 mg of LH/kg in causing ovulation (Foote, Hafs, Staples, Grégoire & Bratton, 1963).…”

Section: Introductionmentioning

confidence: 99%

Ovarian Response and Cleavage Rate of Ova in Control and FSH-Primed Rabbits Receiving Varying Levels of Luteinizing Hormone

Varian¹,

Maurer²,

Foote³

1967

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Summary. The superovulatory response of Dutch-belted does receiving 0\m=.\10,0\m=.\25,0\m=.\50,1\m=.\00,2\m=.\00 and 4\m=.\00mg of LH/kg of body weight following priming with twice daily injections of 0\m=.\50 mg of fsh each for 3 days was examined. The does were inseminated immediately following lh injection. Rabbits given the lowest level of lh did not ovulate, and an average of 1\m=.\0ovulation points was obtained following the 0\m=.\25 mg level of lh. All does receiving \m=ge\0\m=.\50 mg of lh ovulated, with an average of 33\m=.\7,40\m=.\0, 45\m=.\1and 35\m=.\6ovulation points for the 0\m=.\50,1\m=.\00, 2\m=.\00and 4\m=.\00 mg levels, respectively (P>0\m=.\10). Some large unruptured follicles remained in all groups after they received lh.The proportion of cleaved ova collected from fsh-primed does 26, 28 and 30 hr after lh administration was 68, 86 and 91% respectively, whereas in non-primed controls all fertilized ova appeared to have cleaved by 26 hr. This difference is attributed to the longer time required for ovulation to be completed in fsh-primed animals rather than a delay in cleavage rate following fertilization.

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“…On the other hand, Major and Armstrong (1968) (Galton, 1968) and in lactation (Grosvenor and Turner, 1958 Hilliard et al (1963) have shown that ACTH, oxytocin, and vasopressin are ineffective in stimulating the output of progestins in rabbits.…”

Section: Discussionmentioning

confidence: 99%