Gonadal transcriptome analysis of paradise fish Macropodus opercularis to reveal sex-related genes

Liu, Fan; Zhang, Xueling; Wei, Xiaokai; Li, Yu; Liu, Wei; Gan, Guochen; Xiao, Lingling; Wang, Xinyue; Luo, Hui

doi:10.1016/j.cbd.2023.101125

Cited by 4 publications

(1 citation statement)

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“…( Figure 2 A,B). Recent reports have also shown that the number of male-related genes from the mature gonads is much larger than that of female-related genes in other species such as Bombyx mori [ 40 ], Henosepilachna vigintioctopunctata [ 41 ], green mud crab Scylla paramamosain [ 42 ], red-tail catfish Mystus wyckioides [ 43 ], paradise fish Macropodus opercularis [ 44 ], and Hemibarbus maculatus [ 45 ]. This indicates that despite the gene expression profiles varying between species, the overall expression patterns for sex-related genes may be conserved across metazoan species.…”

Section: Discussionmentioning

confidence: 99%

Gonadal Transcriptomic Analysis Reveals Novel Sex-Related Genes in Bactrocera dorsalis

Wang,

Lei,

Lin

et al. 2024

Insects

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Bactrocera dorsalis (Hendel) (Diptera: Tephritidae) is one of the most devastating agricultural pests worldwide due to its high reproductive and invasive abilities. The elucidation of its gonadal developmental characteristics and the identification of sex-related genes will provide a useful genetic basis for reproductive-based pest control. Here, the gonadal transcriptome of B. dorsalis was sequenced, and novel gonad-specific expressed genes were analyzed. A total of 1338, 336, 35, and 479 differentially expressed genes (DEGs) were found in the testis (TE), ovary (OV), female accessory gland (FAG), and male accessory gland (MAG), respectively. Furthermore, 463 highly expressed gonad-specific genes were identified, with the TE having the highest number of specific highly expressed genes, at 402, followed by 51 in the OV, 9 in the MAG, and only 1 in the FAG. Strikingly, approximately half of highly expressed gonad-specific genes were uncharacterized. Then, it was found that 35, 17, 3, 2, and 1 of 202 uncharacterized highly expressed TE-specific genes encoded proteins that contained transmembrane domains, signal peptides, high-mobility group boxes, the zinc finger domain, and the BTB/POZ domain, respectively. Interestingly, approximately 40% of uncharacterized highly expressed gonad-specific genes encoding proteins were not predicted to possess functional motifs or domains. Finally, the spatiotemporal expression and sequence characterization of six novel highly expressed gonad-specific genes were analyzed. Altogether, our findings provide a valuable dataset for future functional analyses of sex-related genes and potential target sites for pest control.

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