Gonadal sex differentiation in Atlantic halibut

Hendry, C. I.; Martin-Robichaud, D. J.; Benfey, Tillmann J.

doi:10.1111/j.1095-8649.2002.tb02438.x

Cited by 31 publications

(17 citation statements)

References 33 publications

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“…Successful sex reversal requires the introduction of hormones prior to gonadal differentiation, which is typically observed in females before males. Based on the results of this study, steroids should be administered to both species beginning at approximately 25 mm TL, shortly after weaning to dry feed, as is also the case for Atlantic halibut (Hendry et al 2002(Hendry et al , 2003. Steroids are easily incorporated into dry fish feeds, suggesting that sex reversal should be easy to achieve in these species once optimal dose and treatment duration are determined (Piferrer 2001).…”

Section: Discussionmentioning

confidence: 99%

Gonadal differentiation in Atlantic cod, Gadus morhua L., and haddock, Melanogrammus aeglefinus (L.)

Chiasson¹,

Benfey²,

Martin-Robichaud³

2008

Acta Icth et Piscat

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. 2008. Gonadal differentiation in Atlantic cod, Gadus morhua L., and haddock, Melanogrammus aeglefinus (L.). Acta Ichthyol. Piscat. 38 (2): 127-133.Background. The purpose of this study was to determine the timing of gonadal differentiation in two gadoids: Atlantic cod, Gadus morhua L., and haddock, Melanogrammus aeglefinus (L.). This information is required to develop a practical protocol for the production of monosex populations of these species for aquaculture. Materials and Methods. Cultured larvae and juveniles were collected weekly, measured (total length; TL), prepared histologically and then examined microscopically for the presence of characteristic stages of gonadal differentiation. Results. In Atlantic cod, undifferentiated gonads were present by 18 mm TL, at 84 days post hatch (dph), and definitive germ cells by 19 mm TL (90 dph). Ovarian cavities were first observed at 27 mm TL (102 dph), and by 35 mm TL (112 dph) anatomical divergence into two types of gonads was clear. In haddock, undifferentiated gonads were observed at 21 mm TL (64 dph) and an ovarian cavity was evident at 29 mm TL (71 dph). Conclusion. Gonadal differentiation in Atlantic cod and haddock occurs at roughly the same size in both species (27 and 29 mm TL, respectively). For successful sex reversal, the administration of steroids should therefore begin at approximately 25 mm TL, shortly after weaning onto dry feed.

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Section: Discussionmentioning

confidence: 99%

Gonadal differentiation in Atlantic cod, Gadus morhua L., and haddock, Melanogrammus aeglefinus (L.)

Chiasson¹,

Benfey²,

Martin-Robichaud³

2008

Acta Icth et Piscat

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“…Some authors (Colombo and Grandi, 1996;Hendry et al, 2002) attribute the increase of primordial germ cells in the undifferentiated gonad to a late migration of these cells from the extragonadal regions justifying the observed absence of the mitotic division of the primordial germ cells, as is seen in C. carpio at this time. The time during which the migration of the primordial germ cells occurs is variable depending on the species studied.…”

Section: The Undifferentiated Gonadmentioning

confidence: 99%

Germline Cysts and the Formation of the Germinal Epithelium During the Female Gonadal Morphogenesis in Cyprinus carpio (Teleostei: Ostariophysi: Cypriniformes)

Mazzoni

Grier

Quagio-Grassiotto

2010

The Anatomical Record

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The formation of both germline cysts and the germinal epithelium is described during the ovary development in Cyprinus carpio. As in the undifferentiated gonad of mammals, cords of PGCs become oogonia when they are surrounded by somatic cells. Ovarian differentiation is triggered when oogonia proliferate and enter meiosis, becoming oocytes. Proliferation of single oogonium results in clusters of interconnected oocytes, the germline cysts, that are encompassed by somatic prefollicle cells and form cell nests. Both PGCs and cell nests are delimited by a basement membrane. Ovarian follicles originate from the germline cysts, about the time of meiotic arrest, as prefollicle cells surround oocytes, individualizing them. They synthesize a basement membrane and an oocyte forms a follicle. With the formation of the stroma, unspecialized mesenchymal cells differentiate, and encompass each follicle, forming the theca. The follicle, basement membrane, and theca constitute the follicle complex. Along the ventral region of the differentiating ovary, the epithelium invaginates to form the ovigerous lamellae whose developing surface epithelium, the germinal epithelium, is composed of epithelial cells, germline cysts with oogonia, oocytes, and developing follicles. The germinal epithelium rests upon a basement membrane. The follicles complexes are connected to the germinal epithelium by a shared portion of basement membrane. In the differentiated ovary, germ cell proliferation in the epithelium forms nests in which there are the germline cysts. Germline cysts, groups of cells that form from a single founder cell and are joined by intercellular bridges, are conserved throughout the vertebrates, as is the germinal epithelium. Anat Rec, 293:1581Rec, 293: -1606

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“…At this stage, the ovarian cavity and mitotic oogonia are visible, but the presumptive testis remains undifferentiated. The first distinguishable spermatogonial cells can be identified at 74 mm L F [Hendry et al, 2002]. In the wild, males mature after 4 years (total length, T L , 55 cm and body weight, BW, 1.7 kg), while females reach sexual maturation after 7 years (average T L = 112-115 cm and BW = 18 kg) [Jákupsstovu and Haug, 1988].…”

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confidence: 99%

“…Samples were obtained from 3 age groups: 2-month-old juveniles (n = 10), 3-year-old (n = 12), and 5-year-old individuals (n = 12). The 3 sex developmental stages represented (1) onset of gonadal formation [Hendry et al, 2002], (2) immature individuals (3-yearold males, average T L = 36.2 cm, BW = 0.98 kg; 3-year-old females, T L = 37.5 cm, BW = 1.02 kg; and 5-year-old females, T L = 73.75 cm, BW = 3.18 kg) and (3) mature males (5-year-old males, T L = 68.17 cm, BW = 2.36 kg) at stage IV testis [Weltzien et al, 2002]. Females at 5 years were immature, in a primary oocyte stage.…”

Section: Husbandry Treatments and Samplingmentioning

confidence: 99%

“…Then they migrate towards the posterior of the abdominal cavity where the presumptive gonad elongates at 21-29 mm L F , at the metamorphosis stage. Gonadal sex differentiation occurs by 38 mm L F , which coincides with the post-metamorphic settled stage [Hendry et al, 2002]. At this stage, the ovarian cavity and mitotic oogonia are visible, but the presumptive testis remains undifferentiated.…”

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confidence: 99%

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Sex-Biased miRNA Expression in Atlantic Halibut (Hippoglossus hippoglossus) Brain and Gonads

Bizuayehu

Joanna

Norberg

et al. 2012

Sex Dev

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The role of miRNA in fish sexual development is not elucidated yet. We profiled miRNAs in gonads and brains of Atlantic halibut using SOLiD sequencing technology. We found tissue- and sexually dimorphic expression of several miRNAs, including miR-29a, miR-34, miR-143, miR-145, miR-202-3p, miR-451, and miR-2188. miR-9 and miR-202 were abundant in brain and gonads, respectively. In the next step, we selected some miRNAs showing differential expression patterns between sexes and performed RT-qPCR on 3 age groups: juveniles, 3-year-, and 5-year-olds. In brains, miR-451 was significantly down-regulated in juveniles compared to adults. let-7a, miR-143, and miR-202-3p were up-regulated in gonads of mature males compared to immature females at the same age. We investigated the effect of suppressing aromatase cytochrome P450 enzyme on miRNA expression at the onset of sex differentiation through masculinization with Fadrozole or 17-α-methyltestosterone. We found significant differences in miRNA expression between masculinized individuals and untreated controls. miR-202-3p was significantly down-regulated in female juveniles compared to male juveniles. The expression levels of let-7a and miR-451 were restored after termination of the masculinization treatment. Our data give a first insight into miRNA involvement in sexual development in teleosts.

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Gonadal sex differentiation in Atlantic halibut

Cited by 31 publications

References 33 publications

Gonadal differentiation in Atlantic cod, <I>Gadus morhua</I> L., and haddock, <I>Melanogrammus aeglefinus</I> (L.)

Gonadal differentiation in Atlantic cod, <I>Gadus morhua</I> L., and haddock, <I>Melanogrammus aeglefinus</I> (L.)

Germline Cysts and the Formation of the Germinal Epithelium During the Female Gonadal Morphogenesis in Cyprinus carpio (Teleostei: Ostariophysi: Cypriniformes)

Sex-Biased miRNA Expression in Atlantic Halibut <b><i>(Hippoglossus hippoglossus)</i></b> Brain and Gonads

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