Gonadal ERα/β, AR and TRPV1 gene expression: Modulation by pain and morphine treatment in male and female rats

Vodo, Stella; Arcelli, Diego; Fiorenzani, Paolo; Meriggiola, Maria Cristina; Butkevich, I. P.; Canio, Clara Di; Mikhailenko, V. A.; Aloisi, Anna Maria

doi:10.1016/j.physbeh.2012.12.014

Cited by 16 publications

(14 citation statements)

References 45 publications

(50 reference statements)

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“…Germ cells, from spermatogonia to spermatozoa, and Sertoli cells possess a complete biochemical machinery to synthesize, transport, degrade and bind endocannabioids, that can affect male reproductive functions [14,57]. Our results on the immunolocalization of TRPV1 in germ cells, and in residual spermatids cytoplasm of experimental rats are in agreement with the findings of other authors who detected TRPV1 channels in the seminiferous tubules of rat testis using RT-PCR [17,20,56,58,59]. Grimaldi et al [20] showed elevated expression level of the TRPV1 gene in all stages of spermatogenesis, with the peak expression in meiotic and postmeiotic cells in mouse testis.…”

Section: Discussionsupporting

confidence: 91%

“…To our knowledge, there is no information on the hormonal regulation of TRPV1 expression in male gonad. Study by Vodo et al [56] showed, that the treatment of male rats with morphine did not produce differences in testosterone and estradiol levels, as it was observed in female rats, and did not influence change in the TRPV1 expression in testis. The inhibitory effect of estradiol on activation of TRPV1 by capsaicin was observed in adult rat nociceptor neurons [61].…”

Section: Discussionmentioning

confidence: 78%

“…TRPV1 can be also activated by vanilloid compounds such as capsaicin, and a number of other ligands [54], and by ambient temperature [55]. TRPV1 was also shown to be expressed in rat testis [17,56]. Germ cells, from spermatogonia to spermatozoa, and Sertoli cells possess a complete biochemical machinery to synthesize, transport, degrade and bind endocannabioids, that can affect male reproductive functions [14,57].…”

Section: Discussionmentioning

confidence: 99%

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The immunoexpression of androgen receptor, estrogen receptors alpha and beta, vanilloid type 1 receptor and cytochrome p450 aromatase in rats testis chronically treated with letrozole, an aromatase inhibitor

Pilutin

Misiakiewicz-Has²,

Kolasa³

et al. 2014

Folia Histochem Cytobiol.

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Abstract:The function of testis is under hormonal control and any disturbance of hormonal homeostasis can lead to morphological and physiological changes. Therefore the aim of the study was to investigate the expression of androgen and estrogen receptors (AR, ERs), vanilloid receptor (TRPV1), cytochrome P450 aromatase (P450arom), as well as apoptosis of cells in testis of adult rats chronically treated with letrozole (LT), a non-steroidal aromatase inhibitor, for 6 months. The testicular tissues were fixed in Bouin's fixative and embedded in paraffin. Immunohistochemistry with monoclonal antibodies (abs) against AR, ERa, P450arom, and polyclonal abs against ERb, TRPV1, caspase-3 was applied. Long-lasting estradiol deficiency, as an effect of LT treatment, produced changes in the morphology of testis and altered the expression of the studied receptors in cells of the seminiferous tubules and rate of cell apoptosis. The immunostaining for AR was found in the nuclei of Sertoli cells and the cytoplasm of spermatogonia and spermatocytes in III-IV stages of the seminiferous epithelium cycle. The intensity of staining for P450arom was lower in the testis of LT-treated rats as compared to control animals. The immunofluorescence of ERa and ERb was observed exclusively in the nuclei of Leydig cells of LT-treated rats. There were no changes in localization of TRPV1, however, the intensity of reaction was stronger in germ cells of the seminiferous epithelium after LT treatment. The apoptosis in both groups of animals was observed within the population of spermatocytes and spermatids in II and III stages of the seminiferous epithelium cycle. In testis of LT-treated rats the immunoexpression of caspase-3 was additionally found in the germ cells in I and IV stages, and Sertoli, myoid and Leydig cells. In conclusion, our results underline the important role of letrozole treatment in the proper function of male reproductive system, and additionally demonstrate that hormonal imbalance can produce the morphological abnormalities in testis.

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Section: Discussionsupporting

confidence: 91%

Section: Discussionmentioning

confidence: 78%

Section: Discussionmentioning

confidence: 99%

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The immunoexpression of androgen receptor, estrogen receptors alpha and beta, vanilloid type 1 receptor and cytochrome p450 aromatase in rats testis chronically treated with letrozole, an aromatase inhibitor

Pilutin

Misiakiewicz-Has²,

Kolasa³

et al. 2014

Folia Histochem Cytobiol.

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“…32,33 In addition to PNS and CNS, TRPV1 mRNA (in some instances functional channels) has also been detected in a number of nonneuronal tissues such as bladder, lungs, keratinocytes, various cell types in dental tissue, fibroblasts, mast cells, hair follicles, blood vessels, testis, and ovary; however, some degree of controversy still remains regarding the extent of expression and function of TRPV1 in these tissues (reviewed in Refs. 20,34). …”

Section: Ion Channels In the Trpv Subfamilymentioning

confidence: 99%

Sensory TRP Channels

Mickle¹,

Shepherd²,

Mohapatra³

2015

Progress in Molecular Biology and Translational Science

119

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Peripheral detection of nociceptive and painful stimuli by sensory neurons involves a complex repertoire of molecular detectors and/or transducers on distinct subsets of nerve fibers. The majority of such molecular detectors/transducers belong to the transient receptor potential (TRP) family of cation channels, which comprise both specific receptors for distinct nociceptive stimuli, as well as for multiple stimuli. This chapter discusses the classification, distribution, and functional properties of individual TRP channel types that have been implicated in various nociceptive and/or painful conditions.

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“…However, growing evidence has demonstrated that estrogens play a crucial role in the regulation of spermatogenesis [5][6][7], acting in the testis through estrogen receptors a (ERa) and b (ERb) [1]. In the rat, both ER forms were expressed in spermatocytes, elongating spermatids, Sertoli cells and Leydig cells [8]. Estradiol plays a regulatory role in rat spermatogenesis initiation [9] and induces apoptosis and drastic decline in round spermatids and spermatozoa [10].…”

Section: Introductionmentioning

confidence: 99%

17β-estradiol effect on testicular β-endorphin expression in Psammomys obesus

Madani¹,

Moudilou²,

Exbrayat³

et al. 2015

Folia Histochem Cytobiol.

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Introduction. Testicular function in the sand rodent Psammomys obesus is subjected to seasonal alternations with a trigger of spermatogenesis in winter and a total quiescence which extends from late spring to summer. The aim of this study was to investigate the distribution of b-endorphin in the testis at the period of winter sexual activity and at its summer regression, and assess the effect of 17b-estradiol treatment on testicular morphology and b-endorphin expression. Material and methods. The adult males were grouped into 4 groups (rest group, sexually active group, rest treated with 17b-estradiol group and controls at sexual rest injected with olive oil, n = 5 in each group). Using anti-serum against b-endorphin, we studied its testicular expression by Western blot and cellular location by immunohistochemical (IHC) method, respectively. Results. We detected by Western blot a peptide of 3.5 kDa molecular weight corresponding to b-endorphin only in sexually resting and control males. The 17b-estradiol treatment induced a clear reduction in the b-endorphin band expression compared with the latter. These results were confirmed by the IHC analysis since b-endorphin was only observed in the testis at sexual rest and in controls, in majority of seminiferous tubules at the level of germ cells. The intensity of IHC labeling was significantly different between spermatogonia and spermatocytes I or round spermatids which revealed the strongest labeling. The intense immunoreactivity was also located in Leydig cells and highly significantly varied compared to the germ cells. The 17b-estradiol treatment decreased significantly the b-endorphin signal in germ cells but not in Leydig cells. Conclusion. The 17b-estradiol treatment induces a repressive effect on seasonal testicular endorphinergic system in P. obesus and this action targets exclusively the germ cells.

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Gonadal ERα/β, AR and TRPV1 gene expression: Modulation by pain and morphine treatment in male and female rats

Cited by 16 publications

References 45 publications

The immunoexpression of androgen receptor, estrogen receptors alpha and beta, vanilloid type 1 receptor and cytochrome p450 aromatase in rats testis chronically treated with letrozole, an aromatase inhibitor

The immunoexpression of androgen receptor, estrogen receptors alpha and beta, vanilloid type 1 receptor and cytochrome p450 aromatase in rats testis chronically treated with letrozole, an aromatase inhibitor

Sensory TRP Channels

17β-estradiol effect on testicular β-endorphin expression in Psammomys obesus

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