Gonadal differentiation and temperature effects on sex determination in the freshwater pike silverside Chirostoma estor Jordan 1880

Corona-Herrera, Guillermo A.; Tello-Ballinas, Juan Antonio; Martı́nez-Palacios, Carlos A.; Strüssmann, Carlos Augusto; Cárdenas-Reygadas, Rodolfo R.; Martínez-Chávez, Carlos Cristian

doi:10.1007/s10641-016-0491-z

Cited by 15 publications

(17 citation statements)

References 32 publications

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“…Significant differences ( p < 0.01) in sex ratios between treatments were confirmed by histological examination using previous standardized criteria for the species (Corona‐Herrera et al . , ). The percentage of male individuals in CS was 40% while 73% in the CL treatment.…”

Section: Resultsmentioning

confidence: 99%

“…Previous studies on C. estor show that embryos exposed to high temperature (29°C) during incubation results in 82% masculinization rates (Corona‐Herrera et al . , ). Future studies will be needed to determine if cumulative effects can be obtained by mixing high temperature and CL treatments in this species and if differences in light quality (source, intensity and spectrum) may produce similar effects.…”

Section: Discussionmentioning

confidence: 98%

“…, 2014; Corona‐Herrera et al . , ). Recent evidence shows that photoperiod can skew sex ratios in L. tenuis (Brown et al .…”

Section: Discussionmentioning

confidence: 99%

“…Immediately afterwards, organisms were fixed in Bouin´s solution (Sigma‐Aldrich), processed by haematoxylin‐eosin‐phloxine standard staining protocol and described according to previous work by Corona‐Herrera et al . ().…”

Section: Methodsmentioning

confidence: 99%

“…Thus, in this study the hypothesis was that continuous light could act as a ROS‐generating stressor that could activate antagonistic defence mechanisms, which could ultimately lead to similar masculinization effects as previously observed with temperature in the same species (Corona‐Herrera et al . , ). To test this hypothesis, C. estor were fertilized and raised under either a simulated natural or a continuous illumination photoperiods up to 12 weeks post hatching (wph).…”

Section: Introductionmentioning

confidence: 97%

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Experimental evidence of masculinization by continuous illumination in a temperature sex determination teleost (Atherinopsidae) model: is oxidative stress involved?

Corona-Herrera

Arranz

Martı́nez-Palacios

et al. 2018

Journal of Fish Biology

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The present study evaluates the influence of continuous light on phenotypic sex ratios in Chirostoma estor, a temperature sex determination animal model. Relative gene expression levels of 5 day old larvae were performed on two early gonad differentiation genes (sox9 and foxl2), two stress axis activation genes (gcr1 and crf) and four reactive oxygen species (ROS) antagonist effector genes (sod2, ucp2, gsr and cat). Two light treatments were applied from fertilization; control (12L:12D) simulated natural photoperiod and a continuous illumination photoperiod. By the end of the trial (12 weeks after hatching), differentiated and normal gonads were clearly identifiable in both treatments by histological observations. Regarding sex ratio, 73% of phenotypic males were found in continuous illumination compared with 40% in controls. Consistently, the sox9 gene (involved in early testis differentiation) showed an over expression in 64% of the individual larvae analysed compared with foxl2 (ovarian differentiation) suggesting a masculinization tendency in continuous illumination. On the other hand, only 36% of individuals showed the same tendency in the control treatment consistent with phenotypic sex ratios found under normal culture conditions. Relative gene expression results did not show significant difference in sod2, ucp2 and gcr1 levels, but cat, gsr and crf showed significantly higher expression levels in the continuous illumination treatment suggesting that both, the stress axis and ROS response mechanisms were activated at this time. This study suggests, a link between continuous light, oxidative stress and environmental sex determination in vertebrates. However, further research is necessary to describe this possible upstream mechanism that may drive some aspects of sexual plasticity in vertebrates.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 98%

“…, 2014; Corona‐Herrera et al . , ). Recent evidence shows that photoperiod can skew sex ratios in L. tenuis (Brown et al .…”

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 97%

See 3 more Smart Citations

Experimental evidence of masculinization by continuous illumination in a temperature sex determination teleost (Atherinopsidae) model: is oxidative stress involved?

Corona-Herrera

Arranz

Martı́nez-Palacios

et al. 2018

Journal of Fish Biology

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Sexual Dimorphism in Pelvic Fin Length of Two Endangered Freshwater Atherinopsid Species with High Aquaculture Potential

et al. 2018

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The endemic Mexican silversides of Lake Pátzcuaro (Pike Silverside Chirostoma estor) and Lake Chapala (Blacknose Silverside C. promelas) are endangered fish species of great socioeconomic importance in the region. Since the last decade, much progress has been made to close the life cycle in captivity and in promoting the aquaculture of these species. The lack of standardized husbandry and broodstock management practices, including sex discrimination, is a bottleneck to the commercial culture of these species. Recent observations have suggested a possible difference between the length of the pelvic fins of males and females. To explore these observations, biometric measurements were taken from Pike Silverside and Blacknose Silverside broodstocks, comprising body weight, SL, distance from the base of the pelvic fin to the genital pore (BPF), and distance from the tip of pelvic fin to the genital pore (TPF). The latter measurements were used to calculate the TPF/BPF ratio. Sex-specific differences were found in BPF, TPF, and TPF/BPF for both species, clearly indicating a sexually dimorphic characteristic not previously reported in any atherinopsid. This study reveals for the first time the presence of a noticeable sexually dimorphic and externally evident trait in two closely related atherinopsid species, which provides a simple morphometric technique for sex discrimination that minimizes manipulation and thus avoids overstressing or killing the fish.

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The orobranchial structures in four neotropical silversides (Teleostei: Atherinopsidae) related with feeding habits

et al. 2019

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Gonadal differentiation and temperature effects on sex determination in the freshwater pike silverside Chirostoma estor Jordan 1880

Cited by 15 publications

References 32 publications

Experimental evidence of masculinization by continuous illumination in a temperature sex determination teleost (Atherinopsidae) model: is oxidative stress involved?

Experimental evidence of masculinization by continuous illumination in a temperature sex determination teleost (Atherinopsidae) model: is oxidative stress involved?

Sexual Dimorphism in Pelvic Fin Length of Two Endangered Freshwater Atherinopsid Species with High Aquaculture Potential

The orobranchial structures in four neotropical silversides (Teleostei: Atherinopsidae) related with feeding habits

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