Gomisin A decreases the LPS-induced expression of iNOS and COX-2 and activation of RIP2/NF-κB in mouse peritoneal macrophages

Jeong, Hyun‐Ja; Han, Ning; Kim, Kyu-Yeob; Choi, Il-Sook; Kim, Hyung-Min

doi:10.3109/08923973.2014.909848

Cited by 26 publications

(19 citation statements)

References 29 publications

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“…10,11 Gomisin A is a small-molecular-weight component contained in the fruit of Schisandra chinensis Baillon, Which has anti-inflammatory and antioxidant activities. [12][13][14] Previous studies suggest that the antioxidant compounds from Schisandra chinensis including schisandrin B, schisandrin C, gomisin J, and gomisin A attenuate oxidative stressinduced by excessive ROS. 15,16 However, the effects of gomisin A on age-related anti-inflammation and anti-oxidation are still unknown.…”

Section: Introductionmentioning

confidence: 99%

Gomisin A modulates aging progress via mitochondrial biogenesis in human diploid fibroblast cells

Kim

Jeong

Han

et al. 2018

Clin Exp Pharma Physio

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Summary Gomisin A from the fruit of Schisandra chinensis has many pharmacological properties, including hepato‐protective, anti‐diabetic, and anti‐oxidative stress. However, the potential benefit of gomisin A is still not well understood, especially in aging progression. Therefore, the aim of this study was to clarify whether the promotion of mitochondrial biogenesis and autophagy of gomisin A affects anti‐aging progression, and its mechanism. Intermediate (PD32) human diploid fibroblast (HDF) cells were brought to stress‐induced premature senescence (SIPS) using hydrogen peroxide. Gomisin A inhibited reactive oxygen species production even in the SIPS‐HDF cells. Gomisin A was also able to attenuate the activity of senescence‐associated β‐galactosidase and the production of pro‐inflammatory molecules in the SIPS as well as aged HDF cells. The antioxidant activity of gomisin A was determined by recovering the Cu/Zn, Mn‐SOD, and HO‐1 expression in the SIPS‐HDF cells. In mechanistic aspect, gomisin A inhibited the mitogen‐activated protein kinase pathway and the translocation of nuclear factor kappa B to the nucleus. In addition, gomisin A promoted the autophagy and mitochondrial biogenesis factors through the translocation of nuclear factor erythroid 2‐related factor‐2, and inhibited aging progression in the SIPS‐HDF cells. In summary, the enhanced properties of mitochondrial biogenesis and autophagy of gomisin A has a benefit to control age‐related molecules against SIPS‐induced chronic oxidative stress, and gomisin A may be a potential therapeutic compound for the enhancement of intracellular homeostasis to aging progression.

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Section: Introductionmentioning

confidence: 99%

Gomisin A modulates aging progress via mitochondrial biogenesis in human diploid fibroblast cells

Kim

Jeong

Han

et al. 2018

Clin Exp Pharma Physio

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“…When macrophages are stimulated with LPS, the production of pro-inflammatory cytokines is markedly increased (Jeong et al, 2014). To study the effect of SKL on the production of proinflammatory cytokines, we performed ELISA.…”

Section: Effects Of Skl On Lps-induced Proinflammatory Cytokine Produmentioning

confidence: 99%

“…The high amount of NO produced following exposure to LPS plays a vital role in increasing inflammatory reactions (Jeong et al, 2014). To examine the effect of SKL on LPS-induced NO production, we carried out Griess reagent method.…”

Section: Effects Of Skl On Lps-induced No Productionmentioning

confidence: 99%

Anti-oxidant and anti-inflammatory effects of Salix Koreensis Andersson in DC. leaf methanol extract in vitro models

Kim¹,

Kim²

2016

TANG [HUMANITAS MEDICINE]

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Oxidative rancidity in foods causes undesirable changes in nutritive value, aroma, flavor, and color. Salix Koreensis Andersson in DC. (SK) has anti-oxidative and anti-inflammatory effects and is traditionally used to treat neuralgia, edema, pain, and inflammatory diseases. However, the regulatory effects of SK on oxidative and inflammatory reactions have not been elucidated. In this context, we scientifically validated the anti-oxidative and anti-inflammatory activities of SK leaf (SKL). The methanol extract of SKL was evaluated for in vitro anti-oxidative activities. SKL showed increased superoxide dismutase (SOD)-like activity and 1, 1-diphenyl-2-picrylhydrazyl radical scavenging activity. The in vitro anti-oxidant and antiinflammatory activities of SKL were also investigated in lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophages. LPS resulted in decreased SOD activities compared with the unstimulated cells, but SKL significantly increased SOD activities reduced by LPS. In addition, LPS-induced nitric oxide, tumor necrosis factor-α, and interleukin-6 productions were significantly and dose-dependently reduced by SKL in RAW264.7 macrophages without inducing cytotoxicity. In conclusion, these results indicate that SKL will be able to be effectively used as a food additive with anti-oxidative and anti-inflammatory effects.

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“…The NF-κB agonist LPS (10 μg/mL) and inhibitor PDTC (10 μM) were used to activate or suppress NF-κB in ATO-treated cells. Previous studies showed that LPS and PDTC at these two dosages can sufficiently activate or inhibit NF-κB without affecting cell growth in many cell lines, including HepG2 cells (Jeong et al, 2014;Song et al, 2014). The result demonstrated that ATO dosedependently decreased cell viability (Fig.…”

Section: Parp-1 Inhibitor 4an Sensitized Ato In Hepg2 Cells and Thismentioning

confidence: 75%

The role of NF-κB in PARP-inhibitor-mediated sensitization and detoxification of arsenic trioxide in hepatocellular carcinoma cells

et al. 2015

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-The therapeutic efficacy of arsenic trioxide (ATO) for treatments of solid tumors is restricted by its drug resistance and chemotoxicity. In this study, we investigated ATO sensitization and detoxification effect of the Poly (ADP ribose) polymerase-1 (PARP-1) inhibitor 4-Amino-1,8-naphthalimide (4AN) in the hepatocellular carcinoma cell line HepG2. We firstly reported that ATO treatment induced the activation of Nuclear factor of κB (NF-κB) and its downstream anti-apoptosis and pro-inflammatory effectors in a PARP-1-dependent manner and thus conferred HepG2 cells with ATO resistance and toxicity. 4AN significantly suppressed the ATO-induced NF-κB activation, which promotes the apoptotic response and alleviates the inflammatory reaction induced by ATO, resulting in sensitization and detoxification against ATO. We also demonstrated that the ATO-induced activation of PARP-1 and NF-κB was closely associated with the oxidative DNA damage mediated by the generated reactive oxygen species (ROS). Furthermore, the attenuation of ATO-induced ROS and the resulting oxidative DNA damage by N-acetyl-L-cysteine (NAC), a potent antioxidant, significantly reduced the activation of PARP-1 and NF-κB in ATO-treated cells. Our study provides novel insights into the mechanism of the PARP-1-mediated NF-κB signaling pathway in ATO resistance and toxicity in anticancer treatments. This study also highlights the application potential of PARP-1 inhibitors in ATO-based anti-cancer treatments and in prevention of NF-κB-mediated therapeutic resistance and toxicity.

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Gomisin A decreases the LPS-induced expression of iNOS and COX-2 and activation of RIP2/NF-κB in mouse peritoneal macrophages

Cited by 26 publications

References 29 publications

Gomisin A modulates aging progress via mitochondrial biogenesis in human diploid fibroblast cells

Gomisin A modulates aging progress via mitochondrial biogenesis in human diploid fibroblast cells

Anti-oxidant and anti-inflammatory effects of Salix Koreensis Andersson in DC. leaf methanol extract in vitro models

The role of NF-κB in PARP-inhibitor-mediated sensitization and detoxification of arsenic trioxide in hepatocellular carcinoma cells

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