Gold Nanostar Colorimetric Detection of Fructosyl Valine as a Potential Future Point of Care Biosensor Candidate for Glycated Haemoglobin Detection

Abstract

Diabetes Mellitus is a growing global concern. The current methods used to detect glycated haemoglobin are precise, however, utilise expensive equipment, reagents and consumables. These are luxuries which rural communities cannot access. The nanotechnology methods which have been developed for glycated haemoglobin detection are predominantly electrochemically based, have complicated lengthy fabrication processes and utilise toxic chemicals. Here a fructosyl amino acid oxidase gold nanostar biosensor has been developed as a potential future point of care biosensor candidate for glycated haemoglobin detection. The workup done on this biosensor showed that it was able to give a spectrophotometric readout and colorimetric result with naked eye detection in blank serum spiked with fructosyl valine.

Electrospun Nanofibers as Chemosensors for Detecting Environmental Pollutants: A Review

“…Its basic principle is the specific interaction between the biomolecules on the sensor and the object to be analyzed [4,[150][151][152][153][154][155][156]. The light signal formed by absorption or emission band changes resulting from chemical interactions is easily detected by fluorescent molecules, with the light signal correlated with the concentration of the analyte [157][158][159][160][161][162][163][164][165]. Nanomaterials are widely used as probe indicators in various chemical tests due to their high sensitivity, wide detection range, and convenient operation.…”

Glycated Hemoglobin and Methods for Its Point of Care Testing

“…In recent years, there has been great progress in the construction of biosensors and similar methods for the HbA 1c assay [ 80 , 81 , 82 , 83 , 84 , 85 , 86 , 87 , 88 , 89 , 90 ]. Various optical and electro-optical sensor methods have been developed in the past few years.…”

Mechanobactericidal, Gold Nanostar Hydrogel-Based Bandage for Bacteria-Infected Skin Wound Healing

“…Thus, they are ideal candidates for photothermal and bioimaging against various forms of cancer . A change in solution color of AuNst has been reported in the presence of glucose oxidase and glucose, thus suggesting its role as an indicator for macromolecule detection . AuNst have until now been used as a photothermal agent because of the high-heat-generating tendency of the nanospikes.…”

“…31 A change in solution color of AuNst has been reported in the presence of glucose oxidase and glucose, thus suggesting its role as an indicator for macromolecule detection. 32 AuNst have until now been used as a photothermal agent because of the highheat-generating tendency of the nanospikes. A report suggested targeted killing of methicillin-resistant Staphylococcus aureus under near-infrared laser radiation on conjugation of AuNst by vancomycin.…”