Gold nanorods for intravital vascular imaging of preneoplastic oral mucosa

Motamedi, Saam; Shilagard, Tuya; Edward, Kert; Koong, Luke Y.; Qui, Suimin; Vargas, Gracie

doi:10.1364/boe.2.001194

Cited by 14 publications

(16 citation statements)

References 25 publications

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“…HE staining did not show a visible difference between the PBS control and the GNR slices of liver tissue after IV administration of GNR-PEG conjugates (Figure 10). The results are consistent with those reported by other groups [32,45] for similar dosage of GNR (around 10-20 mg/kg body mass), and are presented solely as confirmation our GNR-PEG complexes are non-toxic. Silver staining shows that GNR PEG or Ab conjugates have uniform distribution in liver and noticeably higher number of GNR specific conjugates in mouse tumor.…”

Section: Resultssupporting

confidence: 93%

Biocompatible Gold Nanorod Conjugates for Preclinical Biomedical Research

Liopo

Conjusteau²,

Tsyboulski³

et al. 2012

J Nanomedic Nanotechnol

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Gold nanorods with a peak absorption wavelength of 760 nm were prepared using a seed-mediated method. A novel protocol has been developed to replace hexadecyltrimethylammonium bromide on the surface of the nanorods with 16-mercaptohexadecanoic acid and metoxy-poly(ethylene glycol)-thiol, and the monoclonal antibody HER2. The physical chemistry properties of the conjugates were monitored through optical and zeta-potential measurements to confirm surface chemistry changes. The efficiency of the modifications was quantified through measurement of the average number of antibodies per gold nanorod. The conjugates were investigated for different cells lines: BT-474, MCF7, MCF10, MDCK, and fibroblast. The results show successful cell accumulation of the gold nanorod HER2 conjugates in cells with HER2 overexpression. Incubation of the complexes in heparinized mouse blood demonstrated the low aggregation of the metallic particles through stability of the spectral properties, as verified by UV/VIS spectrometry. Cytotoxicity analysis with LDH release and MTT assay confirms strong targeting and retention of functional activity of the antibody after their conjugation with gold nanorods. Silver staining confirms efficient specific binding to BT-474 cells even in cases where the nanorod complexes were incubated in heparinized mouse blood. This is confirmed through in vivo studies where, following intravenous injection of gold nanorod complexes, silver staining reveals noticeably higher rates of specific binding in mouse tumors than in healthy liver. The conjugates are reproducible, have strong molecular targeting capabilities, have long term stability in vivo and can be used in pre-clinical applications. The conjugates can also be used for molecular and optoacoustic imaging, quantitative sensing of biological substrates, and photothermal therapy.

show abstract

Section: Resultssupporting

confidence: 93%

Biocompatible Gold Nanorod Conjugates for Preclinical Biomedical Research

Liopo

Conjusteau²,

Tsyboulski³

et al. 2012

J Nanomedic Nanotechnol

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show abstract

“…The results are consistent with those reported by other groups 22, 23 for similar dosage of GNR (around 10–20 mg/kg body mass), and are presented solely as confirmation our GNR-PEG complexes are non-toxic. We present SS data showing for the first time dynamic accumulation of PEG-GNR (Figure 3) into liver Kupffer cells.…”

Section: Discussionsupporting

confidence: 93%

“…The percentage of dead cells assessed with Trypan blue demonstrated absence of toxicity for PEGylated GNR in a big range of concentrations up to 2.0 nM (IEC-6 cells) after 24 hours of incubation and up to 0.5 nM or 3 × 10 11 GNR/ml (SKBR-3, MDCK and IEC-6 cells) after 48 hours of incubation. This concentration is consistent with reported levels of GNR-PEG in blood or tissue after IV administration in vivo 12, 22, 23 . The change of zeta-potential from positive to negative after GNR PEGylation (Table 3) confirmed the surface chemistry, i.e.…”

Section: Discussionsupporting

confidence: 91%

Highly Purified Biocompatible Gold Nanorods for Contrasted Optoacoustic Imaging of Small Animal Models

Liopo¹,

Conjusteau²,

Чумакова³

et al. 2012

Nanosci Nanotechnol Lett

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We developed a methodology for high yield synthesis of gold nanorods (GNR) with narrow band optical absorption centered at 760 nm. GNR were purified from hexadecyltrimethylammonium bromide (CTAB) and coated with polyethylene glycol (PEG). The molar ratio between GNR and PEG (1÷50000) was optimized to make the conjugate a biocompatible PEG-GNR contrast agent for optoacoustic (OA) imaging. In vitro toxicity studies showed no significant change in survival rates of cultured normal (IEC-6, MDCK) and cancer (SKBR3 and HEPG2) cells after they were incubated with 0.125 to 1.25 nM PEG-GNR solutions. In vivo toxicity studies in nude mice showed no pathological changes in liver after the IV injection of GNR. Significant enhancements of OA contrast in comparison to images of untreated mice were observed 1 hour after the GNR injection in a dose of 20 mg gold per kg of body mass.

show abstract

“…HE staining did not show a visible difference between the PBS control and the GNR slices of liver tissue after IV administration of GNR-PEG conjugates (Figure 10). The results are consistent with those reported by other groups [32,45] for similar dosage of GNR (around 10-20 mg/kg body mass), and are presented solely as confirmation our GNR-PEG complexes are nontoxic. Silver staining shows that GNR PEG or Ab conjugates have uniform distribution in liver and noticeably higher number of GNR specific conjugates in mouse tumor.…”

Section: Resultssupporting

confidence: 93%

Biocompatible Gold Nanorod Conjugates for Preclinical Biomedical Research

Conjusteau¹

2015

J Nanomed Nanotechnol

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Gold nanorods with a peak absorption wavelength of 760 nm were prepared using a seed-mediated method. A novel protocol has been developed to replace hexadecyltrimethylammonium bromide on the surface of the nanorods with 16-mercaptohexadecanoic acid and metoxy-poly(ethylene glycol)-thiol, and the monoclonal antibody HER2. The physical chemistry properties of the conjugates were monitored through optical and zeta-potential measurements to confirm surface chemistry changes. The efficiency of the modifications was quantified through measurement of the average number of antibodies per gold nanorod. The conjugates were investigated for different cells lines: BT-474, MCF7, MCF10, MDCK, and fibroblast. The results show successful cell accumulation of the gold nanorod HER2 conjugates in cells with HER2 overexpression. Incubation of the complexes in heparinized mouse blood demonstrated the low aggregation of the metallic particles through stability of the spectral properties, as verified by UV/VIS spectrometry. Cytotoxicity analysis with LDH release and MTT assay confirms strong targeting and retention of functional activity of the antibody after their conjugation with gold nanorods. Silver staining confirms efficient specific binding to BT-474 cells even in cases where the nanorod complexes were incubated in heparinized mouse blood. This is confirmed through in vivo studies where, following intravenous injection of gold nanorod complexes, silver staining reveals noticeably higher rates of specific binding in mouse tumors than in healthy liver.The conjugates are reproducible, have strong molecular targeting capabilities, have long term stability in vivo and can be used in pre-clinical applications. The conjugates can also be used for molecular and optoacoustic imaging, quantitative sensing of biological substrates, and photothermal therapy.

show abstract

Gold nanorods for intravital vascular imaging of preneoplastic oral mucosa

Cited by 14 publications

References 25 publications

Biocompatible Gold Nanorod Conjugates for Preclinical Biomedical Research

Biocompatible Gold Nanorod Conjugates for Preclinical Biomedical Research

Highly Purified Biocompatible Gold Nanorods for Contrasted Optoacoustic Imaging of Small Animal Models

Biocompatible Gold Nanorod Conjugates for Preclinical Biomedical Research

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