Gold nanorod-based fluorometric ELISA for the sensitive detection of a cancer biomarker

Alex, Sruthi Ann; Chandrasekaran, Natarajan; Mukherjee, Amitava

doi:10.1039/c8nj03467b

Cited by 7 publications

(1 citation statement)

References 27 publications

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“…(a) Selective response of the APMB system to 0.05 ng/mL CEA against other common interferents. The concentrations of the interferents were as encountered in the serum [ 30 ]. The differences in OD 450 between the CEA and interferents are significant, as indicated with two asterisks ( p < 0.01).…”

Section: Resultsmentioning

confidence: 99%

On-bead DNA synthesis triggered by allosteric probe for detection of carcinoembryonic antigen

et al. 2022

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Sensitive quantification of protein biomarkers is highly desired for clinical diagnosis and treatment. Yet, unlike DNA/RNA which can be greatly amplified by PCR/RT-PCR, the amplification and detection of trace amount of proteins remain a great challenge. Here, we combined allosteric probe (AP) with magnetic bead (MB) for assembling an on-bead DNA synthesis system (named as APMB) to amplify protein signals. The AP is designed and conjugated onto the MB, enabling the protein biomarker to be separated and enriched. Once recognizing the biomarker, the AP alters its conformation to initiate DNA synthesis on beads for primary signal amplification. During the DNA synthesis, biotin-dATPs are incorporated into the newly synthesized DNA strands. Then, the biotin-labeled DNA specifically captures streptavidin (STR)–conjugated horseradish peroxidase (HRP), which is used to catalyze a colorimetric reaction for secondary signal amplification. By using carcinoembryonic antigen (CEA) as a protein model, the APMB can quantify protein biomarkers of as low as 0.01 ng/mL. The response values measured by APMB are linearly related to the protein concentrations in the range 0.05 to 20 ng/mL. Clinical examination demonstrated good practicability of the APMB in quantifying serum protein biomarker. The on-bead DNA synthesis could be exploited to improve protein signal amplification, thus facilitating protein biomarker detection of low abundance for early diagnosis. Graphical abstract Supplementary Information The online version contains supplementary material available at 10.1007/s00604-022-05404-4.

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Section: Resultsmentioning

confidence: 99%

On-bead DNA synthesis triggered by allosteric probe for detection of carcinoembryonic antigen

et al. 2022

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Salt-induced gold nanoparticles aggregation lights up fluorescence of DNA-silver nanoclusters to monitor dual cancer markers carcinoembryonic antigen and carbohydrate antigen 125

Chen

et al. 2020

Analytica Chimica Acta

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Layer-by-layer self-assembly of MoS2/PDDA hybrid film in microfluidic chips for ultrasensitive electrochemical immunosensing of alpha-fetoprotein

Zhang

Wang

et al. 2020

Microchemical Journal

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Gold nanorod-based fluorometric ELISA for the sensitive detection of a cancer biomarker

Abstract: A gold nanorod-based fluorometric immunoassay (nanoELIFA) displayed ∼3.5-fold higher sensitivity (amplified signal) when compared to conventional ELIFA.

Cited by 7 publications

References 27 publications

On-bead DNA synthesis triggered by allosteric probe for detection of carcinoembryonic antigen

On-bead DNA synthesis triggered by allosteric probe for detection of carcinoembryonic antigen

Salt-induced gold nanoparticles aggregation lights up fluorescence of DNA-silver nanoclusters to monitor dual cancer markers carcinoembryonic antigen and carbohydrate antigen 125

Layer-by-layer self-assembly of MoS2/PDDA hybrid film in microfluidic chips for ultrasensitive electrochemical immunosensing of alpha-fetoprotein

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