Gold nanorod-assisted near-infrared stimulation of bullfrog sciatic nerve

Mou, Zongxia; You, Mengxian; Wang, Xue

doi:10.1007/s10103-018-2554-1

Cited by 9 publications

(5 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, the penetrating power of infrared light is weak and it is difficult to stimulate deep tissue. 50 , 51 In the orthotopic transplantation tumor model, near-infrared irradiation is unlikely to penetrate skin and reach HCC cells in liver. Therefore, ICG and MLPs containing ICG were not given to mice and we just evaluated the synergistic therapeutic effect of TAIII and DOX on the orthotopic transplantation tumor model.…”

Section: Resultsmentioning

confidence: 99%

Novel Timosaponin AIII-Based Multifunctional Liposomal Delivery System for Synergistic Therapy Against Hepatocellular Carcinoma Cancer

Zhang¹,

Zhang²,

Jiang³

et al. 2021

IJN

View full text Add to dashboard Cite

Introduction As high cholesterol level has been reported to be associated with cancer cell growth and cholesterol is vulnerable to oxidation, the conventional liposomes including cholesterol in the formulation seem to be challenged. Timosaponin AIII (TAIII), as a steroid saponin from Anemarrhena asphodeloides Bunge, possesses a similar structure with cholesterol and exhibits a wide range of antitumor activities, making it possible to develop a TAIII-based liposome where TAIII could potentially stabilize the phospholipid bilayer as a substitution of cholesterol and work as a chemotherapeutic drug as well. Meanwhile, TAIII could enhance the uptake of doxorubicin hydrochloride (DOX) in human hepatocellular carcinoma (HCC) cells and exhibit synergistic effect. Thus, we designed a novel thermally sensitive multifunctional liposomal system composed of TAIII and lipids to deliver DOX for enhanced HCC treatment. Methods The synergistic effects of DOX and TAIII were explored on HCC cells and the tumor inhibition rate of TAIII-based liposomes carrying DOX was evaluated on both subcutaneous and orthotopic transplantation tumor models. TAIII-based multifunctional liposomes were characterized. Results Synergistic HCC cytotoxicity was achieved at molar ratios of 1:1, 1:2 and 1:4 of DOX/TAIII. TAIII-based liposomes carrying a low DOX dose of 2 mg/kg exhibited significantly enhanced antitumor activity than 5 mg/kg of DOX without detected cardiotoxicity on both subcutaneous and orthotopic transplantation tumor models. TAIII-based liposomes were characterized with smaller size than cholesterol liposomes but exhibited favorable stability. Mild hyperthermia generated by laser irradiation accelerated the release of DOX and TAIII from liposomes at tumor site, and cell permeability of TAIII enhanced uptake of DOX in HCC cells. Conclusion The innovative application of TAIII working as bilayer stabilizer and chemotherapeutic drug affords a stable multifunctional liposomal delivery system for synergistic therapy against HCC, which may be referred for the development of other types of saponins with similar property.

show abstract

Section: Resultsmentioning

confidence: 99%

Novel Timosaponin AIII-Based Multifunctional Liposomal Delivery System for Synergistic Therapy Against Hepatocellular Carcinoma Cancer

Zhang¹,

Zhang²,

Jiang³

et al. 2021

IJN

View full text Add to dashboard Cite

show abstract

“…For the stimulation thresholds presented in this study, infrared auditory prostheses are still likely to face significant engineering challenges in safety and power consumption. Reducing the infrared stimulation threshold through techniques such as electrical costimulation and the use of thermal transducer nanoparticles is an active area of research [16,[79][80][81][82][83][84][85][86][87], and may improve the feasibility of infrared prosthesis design.…”

Section: Discussionmentioning

confidence: 99%

Response of primary auditory neurons to stimulation with infrared light in vitro

et al. 2021

View full text Add to dashboard Cite

Objective. Infrared light can be used to modulate the activity of neuronal cells through thermally-evoked capacitive currents and thermosensitive ion channel modulation. The infrared power threshold for action potentials has previously been found to be far lower in the in vivo cochlea when compared with other neuronal targets, implicating spiral ganglion neurons (SGNs) as a potential target for infrared auditory prostheses. However, conflicting experimental evidence suggests that this low threshold may arise from an intermediary mechanism other than direct SGN stimulation, potentially involving residual hair cell activity. Approach. Patch-clamp recordings from cultured SGNs were used to explicitly quantify the capacitive and ion channel currents in an environment devoid of hair cells. Neurons were irradiated by a 1870 nm laser with pulse durations of 0.2–5.0 ms and powers up to 1.5 W. A Hodgkin-Huxley-type model was established by first characterising the voltage dependent currents, and then incorporating laser-evoked currents separated into temperature-dependent and temperature-gradient-dependent components. This model was found to accurately simulate neuronal responses and allowed the results to be extrapolated to stimulation parameter spaces not accessible during this study. Main results. The previously-reported low in vivo SGN stimulation threshold was not observed, and only subthreshold depolarisation was achieved, even at high light exposures. Extrapolating these results with our Hodgkin-Huxley-type model predicts an action potential threshold which does not deviate significantly from other neuronal types. Significance. This suggests that the low-threshold response that is commonly reported in vivo may arise from an alternative mechanism, and calls into question the potential usefulness of the effect for auditory prostheses. The step-wise approach to modelling optically-evoked currents described here may prove useful for analysing a wider range of cell types where capacitive currents and conductance modulation are dominant.

show abstract

“…NINM has been applied in a wide range of in vitro and in vivo targets, with stimulatory effects reported in hippocampal neurons ( Lavoie-Cardinal et al, 2016 ; Lee et al, 2018 ), dorsal root ganglions ( Carvalho-de-Souza et al, 2015 , 2018 ), bullfrog and rat sciatic nerves ( Eom et al, 2014 ; You and Mou, 2017 ; Mou et al, 2018 ), spiral ganglion neurons ( Yong et al, 2014 ), HEK-293 cells ( Martino et al, 2015 ), cortical neurons ( Farah et al, 2013 ; Johannsmeier et al, 2018 ), NG-108-15 cells ( Paviolo et al, 2014 ) and rat astrocytes ( Eom et al, 2017 ). Similar to the inhibitory effect observed in INM (Section 3.2), nanoparticle-mediated photothermal processes have also been shown to inhibit action potential generation and propagation in a wide range of neurons including rat cardiomyocytes ( Wang et al, 2016 ), primary hippocampal neurons ( Yoo et al, 2014 ; Lee et al, 2018 ; Xia and Nyberg, 2018 ) and Aplysia californica motor neurons ( Duke et al, 2013 ; Lothet et al, 2017 ).…”

Section: Nanophotonic Transduction Mechanismsmentioning

confidence: 99%

Nanoparticle-based optical interfaces for retinal neuromodulation: a review

Stoddart,

Begeng,

Tong

et al. 2024

Front. Cell. Neurosci.

View full text Add to dashboard Cite

Degeneration of photoreceptors in the retina is a leading cause of blindness, but commonly leaves the retinal ganglion cells (RGCs) and/or bipolar cells extant. Consequently, these cells are an attractive target for the invasive electrical implants colloquially known as “bionic eyes.” However, after more than two decades of concerted effort, interfaces based on conventional electrical stimulation approaches have delivered limited efficacy, primarily due to the current spread in retinal tissue, which precludes high-acuity vision. The ideal prosthetic solution would be less invasive, provide single-cell resolution and an ability to differentiate between different cell types. Nanoparticle-mediated approaches can address some of these requirements, with particular attention being directed at light-sensitive nanoparticles that can be accessed via the intrinsic optics of the eye. Here we survey the available known nanoparticle-based optical transduction mechanisms that can be exploited for neuromodulation. We review the rapid progress in the field, together with outstanding challenges that must be addressed to translate these techniques to clinical practice. In particular, successful translation will likely require efficient delivery of nanoparticles to stable and precisely defined locations in the retinal tissues. Therefore, we also emphasize the current literature relating to the pharmacokinetics of nanoparticles in the eye. While considerable challenges remain to be overcome, progress to date shows great potential for nanoparticle-based interfaces to revolutionize the field of visual prostheses.

show abstract

Gold nanorod-assisted near-infrared stimulation of bullfrog sciatic nerve

Cited by 9 publications

References 23 publications

Novel Timosaponin AIII-Based Multifunctional Liposomal Delivery System for Synergistic Therapy Against Hepatocellular Carcinoma Cancer

Novel Timosaponin AIII-Based Multifunctional Liposomal Delivery System for Synergistic Therapy Against Hepatocellular Carcinoma Cancer

Response of primary auditory neurons to stimulation with infrared light in vitro

Nanoparticle-based optical interfaces for retinal neuromodulation: a review

Contact Info

Product

Resources

About