Gold Nanoparticles/Thermochromic Composite Film on Screen-Printed Electrodes for Simultaneous Detection of Protein and Temperature

Abstract

In this study, gold nanoparticles and thermochromic composite films modified screen-printed carbon electrodes (TM-AuNPsSPCEs) were developed as a platform for the simultaneous detection of protein and temperature. The TM-AuNPs composited film had better sensitivity resulting from the gold nanoparticles amplification effect. A phase transition model analysis of TM-AuNPs films found that the TM-AuNPs films had three-phase transition intervals (<45˚C, 45˚C to 80˚C and >80˚C) which accommodated the temperature requirements for protein denaturation. When used to detect different concentrations of haemoglobin (Hb) solution, the TM-AuNPs modified SPCEs had a better sensitivity in detecting the different concentrations in comparison to TM and AuNP modified SPCEs which showed no clear sensitivity towards the different Hb concentrations. The dual detection and excellent sensitivity show a good application prospect for the study of the TM-AuNPs composite film.

Nanoparticles Modified Electrodes: Synthesis, Modification, and Characterization—A Review

“…Moreso, TM-AuNPs modified SPCE was more than triple the bare electrodes. For the AuNPs-modified electrode, the peak current was amplified by six-folds, improving the redox peak; hence, the biological sensitivity [99].…”

“…This electrode poisoning was diminished on modifying with SWCNTs, inferring that the latter provides antifouling properties due to faster electron kinetics and no significant shift in the oxidation peaks as shown in Figure 18. where I p is the peak current [99].…”