Gold Nanoparticles Mediate Improved Detection of β-amyloid Aggregates by Fluorescence

Jara-Guajardo, Pedro; Cabrera, Pablo J.; Célis, Freddy; Soler, Mónica; Berlanga, Isadora; Parra-Muñoz, Nicole; Acosta, Gerardo; Alberício, Fernando; Guzmán, Fanny; Campos, Marcelo; Álvarez, Alejandra; Morales-Zavala, Francisco; Kogan, Marcelo J.

doi:10.3390/nano10040690

Cited by 36 publications

(26 citation statements)

References 36 publications

(59 reference statements)

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“…Current studies of gold nanoparticles have focused on their anisotropic forms because adjustments of the size, morphology and other properties can improve specific characteristics of these nanoparticles. The types of gold nanoparticles include nanorods [ 8 , 9 , 10 ], nanoprisms [ 11 , 12 ], nanocubes [ 13 , 14 ], and nanostars [ 15 , 16 , 17 , 18 ].…”

Section: Introductionmentioning

confidence: 99%

Functionalization of Gold Nanostars with Cationic β-Cyclodextrin-Based Polymer for Drug Co-Loading and SERS Monitoring

et al. 2021

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Gold nanostars (AuNSs) exhibit modulated plasmon resonance and have a high SERS enhancement factor. However, their low colloidal stability limits their biomedical application as a nanomaterial. Cationic β-cyclodextrin-based polymer (CCD/P) has low cytotoxicity, can load and transport drugs more efficiently than the corresponding monomeric form, and has an appropriate cationic group to stabilize gold nanoparticles. In this work, we functionalized AuNSs with CCD/P to load phenylethylamine (PhEA) and piperine (PIP) and evaluated SERS-based applications of the products. PhEA and PIP were included in the polymer and used to functionalize AuNSs, forming a new AuNS-CCD/P-PhEA-PIP nanosystem. The system was characterized by UV–VIS, IR, and NMR spectroscopy, TGA, SPR, DLS, zeta potential analysis, FE-SEM, and TEM. Additionally, Raman optical activity, SERS analysis and complementary theoretical studies were used for characterization. Minor adjustments increased the colloidal stability of AuNSs. The loading capacity of the CCD/P with PhEA-PIP was 95 ± 7%. The physicochemical parameters of the AuNS-CCD/P-PhEA-PIP system, such as size and Z potential, are suitable for potential biomedical applications Raman and SERS studies were used to monitor PhEA and PIP loading and their preferential orientation upon interaction with the surface of AuNSs. This unique nanomaterial could be used for simultaneous drug loading and SERS-based detection.

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Section: Introductionmentioning

confidence: 99%

Functionalization of Gold Nanostars with Cationic β-Cyclodextrin-Based Polymer for Drug Co-Loading and SERS Monitoring

et al. 2021

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“…AuNRs-PEG-D1 at concentrations of 0.1 nM and 0.5 nM increased the fluorescence of CRANAD-2 bound to Aβ fibrils (2 and 3-fold, respectively), allowing its detection at μM concentrations. Furthermore, the methodology was validated ex-vivo in brain slices of transgenic mice and allowed the detection of amyloid plaques, which accounts for the potential applicability of this detection technique to living organisms [ 93 ].…”

Section: Plasmonic Biosensors For In Vitro Optical Detection Of Admentioning

confidence: 99%

“…In particular, studies regarding the use of plasmonic NPs for AD imaging are very limited. Some ex-vivo studies have been performed using AuNPs, with potential future applications in AD sensing [ 78 , 93 , 132 ]. However, a major disadvantage of these experiments is that they are not fully representative in antemortem conditions.…”

Section: In Vivo Imaging Detection Platforms Of Ad Using Plasmonicmentioning

confidence: 99%

Plasmonic Nanoparticles as Optical Sensing Probes for the Detection of Alzheimer’s Disease

Oyarzún

Tapia-Arellano

Cabrera

et al. 2021

Sensors

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Alzheimer’s disease (AD), considered a common type of dementia, is mainly characterized by a progressive loss of memory and cognitive functions. Although its cause is multifactorial, it has been associated with the accumulation of toxic aggregates of the amyloid-β peptide (Aβ) and neurofibrillary tangles (NFTs) of tau protein. At present, the development of highly sensitive, high cost-effective, and non-invasive diagnostic tools for AD remains a challenge. In the last decades, nanomaterials have emerged as an interesting and useful tool in nanomedicine for diagnostics and therapy. In particular, plasmonic nanoparticles are well-known to display unique optical properties derived from their localized surface plasmon resonance (LSPR), allowing their use as transducers in various sensing configurations and enhancing detection sensitivity. Herein, this review focuses on current advances in in vitro sensing techniques such as Surface-enhanced Raman scattering (SERS), Surface-enhanced fluorescence (SEF), colorimetric, and LSPR using plasmonic nanoparticles for improving the sensitivity in the detection of main biomarkers related to AD in body fluids. Additionally, we refer to the use of plasmonic nanoparticles for in vivo imaging studies in AD.

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“…Increases in the fluorescence signal for the detection of aggregates that cannot be detected by the single use of CRANAD-2 have been identified. Gold nanoparticles have made it possible to enhance the in vitro and ex vivo detection of amyloid fluorescence aggregates [ 97 ].…”

Section: Inorganic Materials For Overcoming Aβmentioning

confidence: 99%

“…Mediated aggregation and color shift in AuNP suspension, PrP (95)(96)(97)(98)(99)(100)(101)(102)(103)(104)(105)(106)(107)(108)(109)(110), a cellular prion protein AβO-specific binding peptide, weakened AuNP's IFE to CdTe QD fluorescence and restored fluorescence power. The absorption of PrP (95-110) on the AuNP surface was prevented by the unusual interaction between AβOs and PrP (95)(96)(97)(98)(99)(100)(101)(102)(103)(104)(105)(106)(107)(108)(109)(110) in the presence of AβOs. Accumulation of AuNPs was stopped, and the fluorescence intensity of CdTe QDs was again extinguished.…”

Section: Gold Nanoparticles Effects On Amyloid Beta Peptidementioning

confidence: 99%

Potential Role of Nanoparticles in Treating the Accumulation of Amyloid-Beta Peptide in Alzheimer’s Patients

Abbas

2021

Polymers

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The disorder of Alzheimer’s is marked by progressive pathophysiological neurodegeneration. The amino acid peptides in the amyloid plaques found in the brains of people with Alzheimer’s disease (AD) are known as amyloid-beta (Aβ). Current treatments are not curative, and the effects associated with AD are reduced. Improving treatment results involved the targeting of drugs at optimum therapeutic concentration. Nanotechnology is seen as an unconventional, modern technology that plays a key role in the treatment of Alzheimer’s disease. Using nanoparticles, molecular detection, effective drug targeting, and their combination offer high sensitivity. The aim of this review is to shed light on the function and successful role of nanoparticles to resolve Aβ aggregation and thus to help cure Alzheimer’s disease. The analysis divides these nanoparticles into three categories: polymer, lipid, and gold nanoparticles. A thorough comparison was then made between the nanoparticles, which are used according to their role, properties, and size in the procedure. The nanoparticles can prevent the accumulation of Aβ during the efficient delivery of the drug to the cells to treat Alzheimer’s disease. Furthermore, this comparison demonstrated the ability of these nanoparticles to deal efficiently with Alzheimer’s disease. The role of these nanoparticles varied from delivering the drug to brain cells to dealing with the disease-causing peptide.

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Gold Nanoparticles Mediate Improved Detection of β-amyloid Aggregates by Fluorescence

Cited by 36 publications

References 36 publications

Functionalization of Gold Nanostars with Cationic β-Cyclodextrin-Based Polymer for Drug Co-Loading and SERS Monitoring

Functionalization of Gold Nanostars with Cationic β-Cyclodextrin-Based Polymer for Drug Co-Loading and SERS Monitoring

Plasmonic Nanoparticles as Optical Sensing Probes for the Detection of Alzheimer’s Disease

Potential Role of Nanoparticles in Treating the Accumulation of Amyloid-Beta Peptide in Alzheimer’s Patients

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