Gold nanoparticles/horseradish peroxidase encapsulated polyelectrolyte nanocapsule for signal amplification in Listeria monocytogenes detection

Oaew, Sukunya; Charlermroj, Ratthaphol; Pattarakankul, Thitiporn; Karoonuthaisiri, Nitsara

doi:10.1016/j.bios.2012.02.011

Cited by 24 publications

(11 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Similarly, Wang et al [58] used 10 µg/ml of biotinylated anti‐ Listeria antibody along with range of linker molecules to conjugate with gold nanoparticles. Previous studies have reported an immunogenic nanoparticle‐based ELISA where 100 µg/ml of anti‐ L. monocytogenes antibody was used with gold nanoparticle [59]. However in our study, we comparatively used less concentration of antibody (0.25 µg/ml) bound to magnetosomes for detection of LLO in ELISA.…”

Section: Discussionmentioning

confidence: 97%

Development of magnetosomes‐based biosensor for the detection of Listeria monocytogenes from food sample

Sannigrahi¹,

Arumugasamy

Mathiyarasu

et al. 2020

IET nanobiotechnol.

View full text Add to dashboard Cite

Section: Discussionmentioning

confidence: 97%

Development of magnetosomes‐based biosensor for the detection of Listeria monocytogenes from food sample

Sannigrahi¹,

Arumugasamy

Mathiyarasu

et al. 2020

IET nanobiotechnol.

View full text Add to dashboard Cite

“…The HRP/Ab–AuNP complex (Ab*) was prepared as described previously with slight modifications. First, the pH of AuNPs solution was adjusted to 9.0 using 0.01 M NaOH solution, and then 0.1 mg/mL Ab and 1 mg/mL HRPs were added dropwise to 1.0 mL of AuNPs solution at a ratio of 1:10.…”

Section: Methodsmentioning

confidence: 99%

A highly sensitive capillary electrophoresis immunoassay strategy based on dual‐labeled gold nanoparticles enhancing chemiluminescence for the detection of prostate‐specific antigen

Shi

Zhao

et al. 2017

Electrophoresis

View full text Add to dashboard Cite

An enzyme and antibody dual labeled gold nanoparticles enhancing chemiluminescence strategy was developed for highly sensitive CE immunoassay (IA) of prostate-specific antigen (PSA). In this work, gold nanoparticles were labeled with horseradish peroxidase and antiprostate specific antigen-antibody, and used as the marker (Ab ). After PSA (antigen, Ag) was added into the system, a noncompetitive immune reaction was happen between Ab and Ag to form an immune complex (Ag-Ab ). Subsequently, the obtained Ag-Ab and unreacted Ab were separated by CE, and the chemiluminescence intensity of Ag-Ab was used to estimate PSA concentration. The calibration curve showed a good linearity in the range of 0.25-10 ng/mL. Based on a S/N of 3, the detection limit for PAS was estimated to be 0.092 ng/mL. Proposed CE method was applied for PSA quantification in human serum samples from healthy volunteers and patients with prostate cancer. The obtained results demonstrated that the proposed CE method may serve as an alternative tool for clinical analysis of PSA.

show abstract

“…The nanocapsule probe was synthesized using a modified method (Oaew et al, 2012). Briefly, 10 mL AuNPs was centrifuged at 11,360 × g for 30 min and resuspended into 1 mL of Milli-Q purified water.…”

Section: Preparation Of the Nanocapsule Probementioning

confidence: 99%

“…A primary property of this technique was the preparation of nanoparticles with core-independent (Del et al, 2014;Labouta & Schneider, 2010). This technique has attracted intensive attention in various technological fields such as drug (Ariga, Lvov, Kawakami, Ji, & Hill, 2011;Chen et al, 2017;Del et al, 2014;Luo, Venkatraman, & Neu, 2013) and vaccine delivery (Chong et al, 2009), biosensor (Kreft, Javier, Sukhorukov, & Parak, 2007;Xuan, Jia, Jiang, Abdel-Halim, & Zhu, 2012) and bioreactor (Matsusaki, Amemori, Kadowaki, & Akashi, 2011) mostly because they can protect the encapsulated bioactive agents from the surrounding environmental hazards (Balabushevich, Sukhorukov, & Larionova, 2005;Lvov & Caruso, 2001) and encapsulate nanoparticles to load more molecules to enhance the signal amplification of immunoassays (Harshala, Parab, & Ru-Shi Liu, 2009;Oaew, Charlermroj, Pattarakankul, & Karoonuthaisiri, 2012;Trau & Renneberg, 2003).…”

mentioning

confidence: 99%

Polyelectrolyte nanocapsule probe for the determination of imidacloprid in agricultural food samples

Qiao

et al. 2019

Food and Agricultural Immunology

View full text Add to dashboard Cite

Layer-by-layer (LBL) technique is a well-established method for the formation of nanocapsules, which could encapsulate nanoparticles by polyelectrolyte and has received great interest in some areas of research. In this study, colloidal gold nanoparticles (AuNPs) were used as a vehicle for loading horseradish peroxidase (HRP), and AuNPs-HRP was encapsulated by using LBL technique to form a signal amplification nanocapsule probe. Based on the probe, an immunoassay was developed for the detection of imidacloprid in agricultural food samples (rice, pear and cabbage). The IC 50 value of the assay was 0.11 ng mL −1 which was 8-9954 times lower than those of traditional ELISAs and well below the maximum residue limits (MRLs) permitted by the European Union (EU). The recoveries of imidacloprid from three different samples were 80.34-120.84% that had a good correlation (R 2 = 0.9739) with those obtained by the commercial kit. The developed assay could provide a sensitive method for detection of imidacloprid.ARTICLE HISTORY

show abstract

Gold nanoparticles/horseradish peroxidase encapsulated polyelectrolyte nanocapsule for signal amplification in Listeria monocytogenes detection

Cited by 24 publications

References 34 publications

Development of magnetosomes‐based biosensor for the detection of Listeria monocytogenes from food sample

Development of magnetosomes‐based biosensor for the detection of Listeria monocytogenes from food sample

A highly sensitive capillary electrophoresis immunoassay strategy based on dual‐labeled gold nanoparticles enhancing chemiluminescence for the detection of prostate‐specific antigen

Polyelectrolyte nanocapsule probe for the determination of imidacloprid in agricultural food samples

Contact Info

Product

Resources

About