Gold nanoparticles enhance microRNA 31 detection in colon cancer cells after inhibition with chlorogenic acid

Abstract

In the present study, the inhibitory effect of chlorogenic acid (CGA), a phenolic compound with potential antitumor effects, on circulating microRNA 31 (miR-31), was evaluated in RKO colon cancer cells. The capacity of gold nanoparticles (AuNPs) to enhance miR-31 quantification after treatment with CGA was assessed. RKO cells were treated with different concentrations of CGA for 24, 48 and 72 h, after which AuNPs coupled to CD81 were added to the supernatants. Total RNA was extracted, and miR-31 was quantified by reverse transcription-quantitative PCR. The results revealed an 85% decrease in miR-31 level following treatment with 1,000 µM CGA for 72 h, and the highest capacity to detect miR-31 (after treatment and isolation with AuNPs + CD81) was observed at 24 h. Furthermore, CGA decreased the expression of the miR-31 oncogene in an in vitro colon cancer model, and the use of AuNPs enhanced the levels of miRNA detection. The results suggest that miR-31 inhibition is one mechanism by which CGA decreases colon cancer cell proliferation. Moreover, AuNPs can increase the capacity of miR-31 quantification, representing a new strategy to develop non-invasive tools for the molecular diagnosis and monitoring of colon cancer.

Molecular Effects of Iodine-Biofortified Lettuce in Human Gastrointestinal Cancer Cells

“…Indeed, a salicylic acid derivative, anacardic acid, acts as a histone deacetylase inhibitor in human cancer cells [ 26 ]. Another explanation could come from research on polyphenols where one of the major hydroxycinnamic acids in lettuce, chlorogenic acid, was identified as histone acetyltransferase inhibitor [ 27 ], and was shown to inhibit proliferation of colon cancer cells [ 18 ] and ameliorate other diseases [ 19 ] through micro-RNA-dependent mechanism.…”

“…It is believed that altered patterns of DNA methylation play a role in the progression of cancer and pathogenesis of other disease [ 16 ]. Interestingly, polyphenols from lettuce, iodine, and salicylic acid were all reported to exert certain epigenetic effects [ 16 , 17 , 18 , 19 , 20 , 21 , 22 , 23 , 24 , 25 , 26 , 27 ].…”

Chlorogenic Acid: A Systematic Review on the Biological Functions, Mechanistic Actions, and Therapeutic Potentials

“…CGA and its metabolites can increase the levels of pro-caspase-3 and activated caspase-3 in human colon cancer cell lines such as Caco-2 [280]. CGA combined with lactoferrin arrests SW480 cells at the G 0 /G 1 phase and decreases cell viability [281].…”

The Epigenetic Effects of Coffee

“…Thus, coffee suppressed the KRAS signaling pathways including the mitogen-activated protein kinase (MAPK) and phosphatidylinositol 3-kinase (PI3K) signaling pathways and further exerted an inhibitory effect on cell proliferation [ 30 ]. In 2021, Luque-Badillo et al found in vitro that one of the coffee active ingredients, CGA, could decrease colon cancer cell proliferation by inhibiting the expression of the miR-31 oncogene which played a vital role in promoting cell-cycle progression [ 31 ].…”