Gold Nanoparticles dotted Reduction Graphene Oxide Nanocomposite Based Electrochemical Aptasensor for Selective, Rapid, Sensitive and Congener-Specific PCB77 Detection

Wu, Lidong; Lu, Xianbo; Fu, Xiaochen; Wu, Lingxia; Liu, Huan

doi:10.1038/s41598-017-05352-7

Cited by 40 publications

(24 citation statements)

References 25 publications

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“…With this biosensor, the detection limit (LOD) of 0.16 nM was obtained with a sensitivity of 378 nA/nM for the ERBB2 biomarker [ 54 ]. In addition, an AuNP dotted reduction graphene oxide (rGO-AuNP) nanocomposite-based electrochemical aptasensor has been used to selectively detect a concentration of 3,3′4,4′-polychlorinated biphenyls (PCB77) between 1 pg L − 1 and 10 μg L − 1 , with a LOD of 0.1 pg L − 1 [ 55 ]. Moreover, AuNP-GO hybrids have been used as an electrochemical-based biosensor to detect hydrogen peroxide (H 2 O 2 ), with food dynamic responses ranging from 0.1 to 2.3 mM, and an LOD of 0.01 mM [ 57 ].…”

Section: Introductionmentioning

confidence: 99%

Enhanced Plasmonic Biosensors of Hybrid Gold Nanoparticle-Graphene Oxide-Based Label-Free Immunoassay

et al. 2018

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In this study, we propose a modified gold nanoparticle-graphene oxide sheet (AuNP-GO) nanocomposite to detect two different interactions between proteins and hybrid nanocomposites for use in biomedical applications. GO sheets have high bioaffinity, which facilitates the attachment of biomolecules to carboxyl groups and has led to its use in the development of sensing mechanisms. When GO sheets are decorated with AuNPs, they introduce localized surface plasmon resonance (LSPR) in the resonance energy transfer of spectral changes. Our results suggest a promising future for AuNP-GO-based label-free immunoassays to detect disease biomarkers and rapidly diagnose infectious diseases. The results showed the detection of antiBSA in 10 ng/ml of hCG non-specific interfering protein with dynamic responses ranging from 1.45 nM to 145 fM, and a LOD of 145 fM. Considering the wide range of potential applications of GO sheets as a host material for a variety of nanoparticles, the approach developed here may be beneficial for the future integration of nanoparticles with GO nanosheets for blood sensing. The excellent anti-interference characteristics allow for the use of the biosensor in clinical analysis and point-of-care testing (POCT) diagnostics of rapid immunoassay products, and it may also be a potential tool for the measurement of biomarkers in human serum.Electronic supplementary materialThe online version of this article (10.1186/s11671-018-2565-7) contains supplementary material, which is available to authorized users.

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Section: Introductionmentioning

confidence: 99%

Enhanced Plasmonic Biosensors of Hybrid Gold Nanoparticle-Graphene Oxide-Based Label-Free Immunoassay

et al. 2018

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“…This vial was purged with high purity argon (99.999%) for 20 min before being sealed. The ball milling process was carried out at 450 rpm for 20 hours to yield GO 20 . Subsequently, 25 mg of GO was dispersed in 50 mL of deionized water in a round-bottom flask, and 20 mg of N,N’-dicyclohexylcarbodiimide (NDC) was added at room temperature before ultrasonication of the mixture for 30 min.…”

Section: Methodsmentioning

confidence: 99%

Reduced Graphene Oxide-Gold Nanoparticle Nanoframework as a Highly Selective Separation Material for Aflatoxins

Guo

Fan

et al. 2017

Sci Rep

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Graphene-based materials have been studied in many applications, owing to the excellent electrical, mechanical, and thermal properties of graphene. In the current study, an environmentally friendly approach to the preparation of a reduced graphene oxide-gold nanoparticle (rGO-AuNP) nanocomposite was developed by using L-cysteine and vitamin C as reductants under mild reaction conditions. The rGO-AuNP material showed a highly selective separation ability for 6 naturally occurring aflatoxins, which are easily adsorbed onto traditional graphene materials but are difficult to be desorbed. The specificity of the nanocomposite was evaluated in the separation of 6 aflatoxin congeners (aflatoxin B1, aflatoxin B2, aflatoxin G1, aflatoxin G2, aflatoxin M1 and aflatoxin M2) from 23 other biotoxins (including, ochratoxin A, citrinin, and deoxynivalenol). The results indicated that this material was specific for separating aflatoxin congeners. The synthesized material was further validated by determining the recovery (77.6–105.0%), sensitivity (limit of detection in the range of 0.05–0.21 μg kg−1), and precision (1.5–11.8%), and was then successfully applied to the separation of aflatoxins from real-world maize, wheat and rice samples.

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“…Wu et al reported gold nanoparticle dotted reduced graphene oxide (rGO-AuNP) which was used as a substrate for an aptamer biosensor [51]. Wang et al reported a responsive acetylcholinesterase biosensor in combination with a screen-printed electrode modified with iron oxide nanoparticle [52].…”

Section: Graphenementioning

confidence: 99%

Electrochemical biosensors: perspective on functional nanomaterials for on-site analysis

2020

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Background: The electrochemical biosensor is one of the typical sensing devices based on transducing the biochemical events to electrical signals. In this type of sensor, an electrode is a key component that is employed as a solid support for immobilization of biomolecules and electron movement. Thanks to numerous nanomaterials that possess the large surface area, synergic effects are enabled by improving loading capacity and the mass transport of reactants for achieving high performance in terms of analytical sensitivity. Main body: We categorized the current electrochemical biosensors into two groups, carbon-based (carbon nanotubes and graphene) and non-carbon-based nanomaterials (metallic and silica nanoparticles, nanowire, and indium tin oxide, organic materials). The carbon allotropes can be employed as an electrode and supporting scaffolds due to their large active surface area as well as an effective electron transfer rate. We also discussed the non-carbon nanomaterials that are used as alternative supporting components of the electrode for improving the electrochemical properties of biosensors. Conclusion: Although several functional nanomaterials have provided the innovative solid substrate for high performances, developing on-site version of biosensor that meets enough sensitivity along with high reproducibility still remains a challenge. In particular, the matrix interference from real samples which seriously affects the biomolecular interaction still remains the most critical issues that need to be solved for practical aspect in the electrochemical biosensor. Recently, various electrochemistry-driven biosensing methods have been introduced for simple and miniaturized analytical devices for on-site analysis. This trend can be applied to replace the commercial lab instruments manufactured by the renowned in vitro diagnosis

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Gold Nanoparticles dotted Reduction Graphene Oxide Nanocomposite Based Electrochemical Aptasensor for Selective, Rapid, Sensitive and Congener-Specific PCB77 Detection

Cited by 40 publications

References 25 publications

Enhanced Plasmonic Biosensors of Hybrid Gold Nanoparticle-Graphene Oxide-Based Label-Free Immunoassay

Enhanced Plasmonic Biosensors of Hybrid Gold Nanoparticle-Graphene Oxide-Based Label-Free Immunoassay

Reduced Graphene Oxide-Gold Nanoparticle Nanoframework as a Highly Selective Separation Material for Aflatoxins

Electrochemical biosensors: perspective on functional nanomaterials for on-site analysis

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